A New Approach to the Molecular Characterization of the C-ANCA Antigen in Wegener’s Granulomatosis

  • Lecy Bleil
  • Thomas H. Winkler
  • Holger K. Fehr
  • Eckehard K. F. Bautz
  • Joachim R. Kalden
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 336)

Summary

Classic anti-neutrophil cytoplasmic antibodies (c-ANCA) specific for constituents of neutrophil primary granules and monocyte lysosomes have been implicated in the pathogenesis of Wegener’s Granulomatosis (WG). The revised amino-terminal sequence of Proteinase 3 (PR-3) as ANCA antigen, suggested that PR-3 is identical to myeloblastin (MBN). As it has been proposed that autoantibodies recognize a conformational epitope on c-ANCA, prokaryotic expressed protein might not be recognized by the patients sera. Therefore we set up an in vitro translation in an eukaryotic cell-free system using an internal ATG (amino acid 15 of the mature protein).

Keywords

Polyarteritis Nodosa Neutrophil Granulocyte Conformational Epitope Bromphenol Blue Rabbit Reticulocyte Lysate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Lecy Bleil
    • 1
  • Thomas H. Winkler
    • 1
  • Holger K. Fehr
    • 1
  • Eckehard K. F. Bautz
    • 2
  • Joachim R. Kalden
    • 1
  1. 1.Institute of Clinical Immunology and Rheumatology Department of Medicine IIIUniversity of Erlangen-NürnbergGermany
  2. 2.Institute f. Molecular GeneticsUniversity of HeidelbergGermany

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