Methods of Detection of Anticathepsin G Autoantibodies in Human

  • P. Lesavre
  • P. Nusbaum
  • L. Halbwachs-Mecarelli
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 336)


Anticathepsin G antibodies have been detected by using three different methods. i) Binding to azurophilic granules constituants after separation of purified α-granules on Matrex gel Orange A chromatography according to Kao. ii) Binding to azurophilic granules freezed and thawed after coating on ELISA plates. iii) Binding to purified cathepsin G in ELISA assay.

Anticathepsin G antibodies were observed patient’s sera with ulcerative colitis, primary sclerosing cholangitis, primary biliary cirrhosis and autoimmune hepatitis but not in controls or patients with chronic viral hepatitis or vasculitis.


Ulcerative Colitis Primary Biliary Cirrhosis Primary Sclerosing Cholangitis Autoimmune Hepatitis Chronic Viral Hepatitis 
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  1. 1.
    Nielsen H, Wiik A, Elmgreen J. Granulocyte specific antinuclear antibodies in ulcerative colitis. Acta Path Microbiol Immunol Scand (Sect C) 1983; 91: 23–6.Google Scholar
  2. 2.
    Snook JA, Chapman RW, Fleming K, Jewell DP. Anti-neutrophil nuclear antibody in ulcerative colitis, Crohn’s disease and primary sclerosing cholangitis. Clin Exp Immunol 1989; 76: 30–3.PubMedGoogle Scholar
  3. 3.
    Seibold F, Weber P, Klein R, Berg PA, Wiedmann KH. Clinical significance of antibodies against neutrophils in patients with inflammatory bowel disease and primary sclerosing cholangitis. Gut 1992; 33: 657–62.PubMedCrossRefGoogle Scholar
  4. 4.
    Cambridge G, Rampton DS, Stevens TRJ, McCarthy DA, Kamm M, Leaker B. Anti-neutrophil antibodies in inflammatory bowel disease: prevalence and diagnostic role. Gut 1992, 33: 668–74.PubMedCrossRefGoogle Scholar
  5. 5.
    Halbwachs-Mecarelli L, Nusbaum P, Noël LH, Reumaux D,Erlinger S, Grünfeld JP, Lesavre P. Antineutrophil cytoplasmic antibodies (ANCA) directed against Cathepsin G in ulcerative colitis, Crohn’s disease and primary sclerosing cholangitis. Clin. Exp. Immunol. 1992 90: 79–84.PubMedCrossRefGoogle Scholar
  6. 6.
    Reumaux D, Colombel JF, Cortot A, Noël LH, Lesavre P, Dutuilleul P. Antineutrophil cytoplasmic autoantibodies directed against cathepsin G in sera from patients with inflammatory bowel disease. (Abstr.). Gut 1991, 32, Al218.Google Scholar
  7. 7.
    Gayno S, Mecarelli L, Lesavre P, Degos F, Erlinger S. Antineutrophil cytoplasmic antibodies (ANCA) directed against cathepsin G in primary sclerosing cholangitis (PSC) and chronic liver disease. (Abstr.). Hepatology 1991, 14, 193A.Google Scholar
  8. 8.
    Borregaard N, Heiple JM, Simons ER, Clark RA. Subcellular localization of the b-cytochrome component of the human neutrophil microbicidal oxidase: translocation during activation. J Cell Biol 1983; 97: 52–61.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • P. Lesavre
    • 1
  • P. Nusbaum
    • 1
  • L. Halbwachs-Mecarelli
    • 1
  1. 1.INSERM U90 and Department of NephrologyNecker HospitalParis Cédex 15France

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