Anti-Neutrophil Cytoplasm Antibodies (ANCA) Increase Neutrophil Adhesion to Cultured Human Endothelium
ANCA have been demonstrated to activate normal neutrophils in vitro. This study demonstrates that ANCA positive IgG and Fab2 fragments increase the adhesion of normal neutrophils to cultured human umbilical vein endothelium (HUVEC), and that preincubation of the endothelial monolayers with tumour necrosis factor (TNF) to activate the endothelium enhances this effect. Preincubation of neutrophils with antiCD18 monoclonal antibodies inhibited adhesion, suggesting that the observed effect was integrin mediated.
KeywordsFab2 Fragment Neutrophil Adhesion Normal Neutrophil Recombinant Human Tumour Necrosis Factor Human Umbilical Vein Endothelium
Unable to display preview. Download preview PDF.
- 3.Ewert, B.H., Jennette, J.C.,and Falk, R.J., 1992, Anti-myeloperoxidase antibodies stimulate neutrophils to damage human endothelial cells. Kidneylnt 41: 375–383.Google Scholar
- 4.Weiss, S.J., 1987, Tissue destruction by neutrophils. N. Engl. J Med. 320: 365–375.Google Scholar
- 7.Smith, C.W., Marlin, S.D., Rothlein, R., Toman, C. and Anderson, D.C. 1989, Cooperative interactions of LFA-1 and Mac-1 with intercellular adhesion molecule-1 in facilitating adherance and transendothelial migration of human neutrophils in vitro. J clin. Invest. 83: 2008–2012.PubMedCrossRefGoogle Scholar