Antineutrophil Cytoplasmic Autoantibodies (ANCA) Recognizing a Recombinant Myeloperoxidase Subunit

  • Elisete Pedrollo
  • Lecy Bleil
  • Friedlinde A. Bautz
  • Joachim R. Kalden
  • Ekkehard K. F. Bautz
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 336)

Summary

Sera from 76 patients with diverse vasculitis, systemic lupus erythematosus (SLE) and hydralazine-induced lupus (HiL) were analysed by ELISA for their reactivity with native, reduced or urea-denatured MPO, respectively, as well as with bacterially expressed heavy and light subunits of MPO. All sera (n=20) recognizing native MPO showed a positive reaction with reduced MPO, while 12 recognized the denatured protein. Most of the linear epitopes are located in the light subunit, since 9 MPO-positive sera recognized significantly the bacterially expressed, denatured light subunit, while only one serum recognized the bacterially expressed heavy subunit purified under denaturing conditions.

Keywords

Systemic Lupus Erythematosus Systemic Vasculitis Crescentic Glomerulonephritis Linear Epitope Antineutrophil Cytoplasmic Autoantibody 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Elisete Pedrollo
    • 1
  • Lecy Bleil
    • 2
  • Friedlinde A. Bautz
    • 1
  • Joachim R. Kalden
    • 2
  • Ekkehard K. F. Bautz
    • 1
  1. 1.Institute of Molecular GeneticsUniversity of HeidelbergHeidelbergGermany
  2. 2.Institute of Rheumathology and Clinical ImmunologyUniversity of Erlangen-NürnbergErlangenGermany

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