Antineutrophil Cytoplasmic Autoantibodies (ANCA) Recognizing a Recombinant Myeloperoxidase Subunit
Sera from 76 patients with diverse vasculitis, systemic lupus erythematosus (SLE) and hydralazine-induced lupus (HiL) were analysed by ELISA for their reactivity with native, reduced or urea-denatured MPO, respectively, as well as with bacterially expressed heavy and light subunits of MPO. All sera (n=20) recognizing native MPO showed a positive reaction with reduced MPO, while 12 recognized the denatured protein. Most of the linear epitopes are located in the light subunit, since 9 MPO-positive sera recognized significantly the bacterially expressed, denatured light subunit, while only one serum recognized the bacterially expressed heavy subunit purified under denaturing conditions.
KeywordsSystemic Lupus Erythematosus Systemic Vasculitis Crescentic Glomerulonephritis Linear Epitope Antineutrophil Cytoplasmic Autoantibody
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