Abstract
In the peripheral nervous system nerve growth factor (NGF) is essential for the development and survival of sympathetic and sensory neurons (Greene and Shooter, 1980). Recent studies have indicated that NGF also plays an important role in the development and survival of cholinergic neurons in the basal forebrain (Gnahn et al., 1983; Hatanaka et al., 1988). NGF is a target-derived neurotrophic factor, and therefore is secreted by the target tissue near the nerve terminal. The first step of NGF action is the binding to its receptor. It is known that there are two forms of the NGF receptors, the low-and high-affinity receptors, which bind NGF with Kd values of 10−9 M and 10−11 M, respectively (Misko et al., 1988). Experimental evidence strongly suggests that biological responsiveness to NGF is dependent upon interactions with the high-affinity NGF receptor (Misko et al., 1988). cDNA clones for the low-affity NGF receptor (p75LNGFR) were obtained and sequenced (Johnson et al., 1986; Radeke et al., 1987). The amino acid sequence of the p75LNGFR predicts that the cytoplasmic domain lacks a typical structure for cellular signal transduction like that of tyrosine kinase.
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References
Cavicchioli, L., Flanigan, T.P., Vantini, G., Fusco, M., Polato, P., Taffano, G., Walsh, F.S., and Leon, A., 1989, NGF amplifies expression of NGF receptor messenger RNA in forebrain cholinergic neurons of rats, Eur. J. Neurosci. 1: 258–262.
Chomczynski, P., and Sacchi, N., 1987, Single-step method of RNA isolation by acid guanidium thiocyanatephenol-chloroform extraction, Anal. Biochem. 162: 156–159.
Doherty, P., Seaton, P., Flanigan, T.P., and Walsh, F.S., 1988, Factors controlling the expression of the NGF receptors in PC12 cells, Neurosci. Leu. 92: 222–227.
Gnahn, H., Hefti, F., Heumann, R., Schwab, M.E., and Thoenen, H., 1983, NGF-mediated increase of choline acetyltransferase (ChAT) in the neonatal rat forebrain: evidence for a physiological role of NGF in the brain, Dey. Brain Res. 9: 45–52.
Greene, L.A., and Shooter, E.M., 1980, The nerve growth factor: biochemistry, synthesis, and mechanism of action, Ann. Rev. Neurosci. 3: 353–402.
Hatanaka, H., 1981, Nerve growth factor-mediated stimulation of tyrosine hydroxylase activity in a clonal pheochromocytoma cell line, Brain Res. 222: 225–233.
Hatanaka, H., and Tsukui-Tamagawa, H., 1984, Rapid responsive induction of tyrosine hydroxylase in rat pheochromocytoma cells by nerve growth factor, 3rd Int. Cong. Biol., Tokyo.
Hatanaka, H., Tsukui, H., and Nihonmatcu, I., 1988, Developmental change in the nerve growth factor action from induction of choline acetyltransferase to promotion of cell survival in cultured basal forebrain cholinergic neurons from postnatal rats, Dev. Brain Res. 39: 85–95.
Hempstead, B.L., Martin-Zanca, D., Kaplan, D.R., Parada, L.F., and Chao, M.V., 1991, High-affinity NG F binding requires coexpression of the trk proto-oncogene and the low-affinity NGF receptor, Nature 350: 678–683.
Holtzman, D.M., Li, Y., Parada, L.F., Kinsman, S., Chen, C.-K., Valletta, J.S., Zhou, J., Long, J.B., and Mobley, W.C., 1992, p140“k mRNA marks NGF-responsive forebrain neurons: evidence that gene expression is induced by NGF, Neuron 9: 465–478.
Ikenaka, K., Nakahira, K., Takayama, C., Wada, K., Hatanaka, H., and Mikoshiba, K., 1990, Nerve growth factor rapidly induces expression of the 68-kDa neurofilament gene by posttranscriptional modification in PC12h-R cells, J. Biol. Chem. 265: 19780–19785.
Johnson, D., Lanahan, A., Buck, C.R., Sehgal, A., Morgan, C., Mercer, E., Bothwell, M., and Chao, M., 1986, Expression and structure of the human NGF receptor, Cell 47: 545–554.
Klein, R., Jing, S., Nanduri, V., O’Rourke, E., and Barbacid, M., 1991, The trk proto-oncogene encodes a receptor for nerve growth factor, Cell 65: 189–197.
Kojima, M., Takahashi, N., Ikeuchi, T., and Hatanaka, H., 1992, Nerve growth factor (NGF)-mediated up-regulation of low-affinity NGF receptor gene expression in cultured basal forebrain cholinergic neurons from postnatal 3-day-old rats, Mol. Brain Res. 16: 267–273.
Lindsay, R.M., Shooter, E.M., Radeke, M.J., Misko, T.P., Dechant, G., Thoenen, H., and Lindholm, D., 1990, Nerve growth factor regulates expression of the nerve growth factor receptor gene in adult sensory neurons, Eur. J. Neurosci. 2: 389–396.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., 1951, Protein measurement with the Folin phenol reagent, J. Biol. Chem. 193: 265–275.
Miller, F.D., Mathew, T.C., and Toma, J.G., 1991, Regulation of nerve growth factor receptor gene expression by nerve growth factor in the developing peripheral nervous system, J. Cell Biol. 112: 303–312.
Misko, T.P., Radeke, M.J., and Shooter, E.M., 1988, Molecular aspects of the rat nerve growth factor receptor, in: “Neural Development and Regeneration,” A. Gorio, J.R. Perez-Polo, J. de Vellis and B. Haber, eds., Springer, Berlin.
Radeke, M.J., Misko, T.P., Hsu, C., Herzenberg, L.A., and Shooter, E.M., 1987, Gene transfer and molecular cloning of the rat nerve growth factor receptor, Nature 325: 593–597.
Ringstedt, T., Lagercrantz, H., and Persson, H., 1993, Expression of members of the trk family in developing postnatal rat brain, Dev. Brain Res. 72: 119–131.
Verge, V.M.K., Merlio, J.-P., Grondin, J., Emfos, P., Persson, H., Riopelle, R.J., Hokfelt, T., and
Richardson, P.M., 1992, Colocalization of NGF binding sites, trk mRNA and low-affinity NGF receptor mRNA in primary sensory neurons: responses to injury and infusion of NGF, J. Neurosci. 12: 4011–4022.
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Ikeuchi, T., Takahashi, N., Kojima, M., Hatanaka, H. (1995). Regulation of Expression of the Low-Affinity NGF Receptor (p75LNGFR) Gene in Clonal Rat Pheochromocytoma PC12h-R Cells and Cultured Rat Basal Forebrain Cholinergic Neurons: Its Relation to Expression of the High-Affinity NGF Receptor (trkA) Gene. In: Hanin, I., Yoshida, M., Fisher, A. (eds) Alzheimer’s and Parkinson’s Diseases. Advances in Behavioral Biology, vol 44. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9145-7_93
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DOI: https://doi.org/10.1007/978-1-4757-9145-7_93
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