Acetylcholinesterase is Not a Protease: Implications for Alzheimer’s Disease
Acetylcholinesterase (AChE) has been alleged for over a decade to possess a protease activity. The activity was first described in purified eel AChE from commercial suppliers and bovine serum AChE (Chubb et al., 1980; Chubb et al., 1983). Two protease activities appear to reside with the AChE, a trypsin-like endopeptidase and a carboxypeptidase (Small et al., 1987; Small, 1988). The protease activity is difficult to remove from the AChE indicating that it is either tightly bound or intrinsic to the enzyme (Small, 1989; Small, 1990). However, a recent report suggests that bovine serum AChE is reversibly associated with a protease activity (Michaelson and Small, 1993).
KeywordsHigh Performance Liquid Chromatography Protease Activity AChE Activity Velocity Sedimentation Electric Organ
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