Amyloidogenic Fragments of Amyloid Precursor Protein in Cells Cultured under Leupeptin

  • Kayo Tsuzuki
  • Ryo Fukatsu
  • Yuji Takamaru
  • Nobuhiro Fujii
  • Naohiko Takahata
Part of the Advances in Behavioral Biology book series (ABBI, volume 44)


The extracellular deposition of β/A4 protein is the most characteristic neuropathological change observed in the brain of patients suffering from Alzheimer’s disease. The accumulation of β/A4 protein is notable at an early stage of the disease, and increases to a high extent during the progression of the disease which, in turn, is thought to be responsible for generation of neurofibrillary tangles leading to neuronal cell death. The β/A4 protein is a proteolytically cleaved product of receptor-like integral transmembrane protein, the amyloid precursor protein (APP).1,2,3 The mechanism and subcellular compartment of β/A4 protein production are still unknown.


Amyloid Precursor Protein Microsomal Fraction Mitochondrial Fraction Amyloid Precursor Protein Processing Sucrose Density Gradient Centrifugation 
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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Kayo Tsuzuki
    • 1
  • Ryo Fukatsu
    • 2
  • Yuji Takamaru
    • 3
  • Nobuhiro Fujii
    • 1
  • Naohiko Takahata
    • 2
  1. 1.Department of MicrobiologyJapan
  2. 2.Department of NeuropsychiatrySapporo Medical University School of MedicineJapan
  3. 3.Department of Psychiatry and NeurologyHokkaido University School of MedicineSapporo 060Japan

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