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The Complete Enzymic Hydrolysis of Crosslinked Proteins

  • Brian Milligan
  • Leo A. Holt
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 86)

Abstract

Procedures used for the complete enzymic hydrolysis of proteins are reviewed. The successful application of complete enzymic hydrolysis in the detection of naturally occurring isopeptide crosslinks and various other types of chemically introduced crosslinks is described. The method may fail if the level of crosslinking is too high, or if crosslinking is accompanied by racemization. Although it is usual to cleave disulphide cross-links prior to enzymic digestion, the necessity for this in all cases is questioned.

Keywords

Hydrolysis Proline Lysine Glutamine Tryptophan 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Liu, T.S., and Chang, Y.H. (1971). J. Biot. Chem., 246, 2842.Google Scholar
  2. 2.
    Davis, N.C., and Smith, E.L. (1957). J. Biol. Chem., 224, 261.PubMedGoogle Scholar
  3. 3 .
    Hill, R.L., and Schmidt, W.R. (1962). J. Biol. Chem.,237Google Scholar
  4. 4.
    Milligan, B., Holt, L.A., and Caldwell, J.B. (1971). Appt. Polym. Symp., No. 18, 113.Google Scholar
  5. 5.
    Holt, L.A., Milligan, B., and Roxburgh, C.M. (1971). Aust. J. Biol. Sci., 24, 509.PubMedGoogle Scholar
  6. 6.
    Cole, M., Fletcher, J.C., Gardner, K.L., and Corfield, M.C. (1971). AppZ. Polym. Symp., No. 18, 147.Google Scholar
  7. 7.
    Schmitz, I., Baumann, H., and Zahn, H. (1976). ‘Proc. Int. Wool Text. Res. Conf., 5th, Aachen, 1975’ in “Schriftenr. Dtsch. Wollforsch. Inst. Tech. Hochschule Aachen”, in press.Google Scholar
  8. 8.
    Bensusan, H.B., Dixit, S.N., and McKnight, S.D. (1971). Biochim. Biophys. Acta, 251, 100.CrossRefPubMedGoogle Scholar
  9. 9.
    Bensusan, H.B. (1972). Biochim. Biophys. Acta, 285, 447.CrossRefPubMedGoogle Scholar
  10. 10.
    Pisano, J.J., Finlayson, J.S., and Peyton, M.P. (1968). Science, 160, 892.CrossRefPubMedGoogle Scholar
  11. 11.
    Matacid, S. and Loewy, A.G. (1968). Biochem. Biophys. Res. Commun., 30, 356.CrossRefGoogle Scholar
  12. 12.
    Lorand, L., Downey, J., Gotoh, T., Jacobsen, A., and Tokura, S. (1968). Biochem. Biophys. Res. Commun., 31, 222.CrossRefGoogle Scholar
  13. 13.
    Asquith, R.S., Otterburn, M.S., Buchanan, J.H., Cole, M., Fletcher, J.C., and Gardner, K.L. (1970). Biochim. Biophys. Acta, 207, 342.Google Scholar
  14. 14.
    Harding, H.W.H. and Rogers, G.E. (1972). Biochim. Biophys. Acta, 257, 37.CrossRefPubMedGoogle Scholar
  15. 15.
    Harding, H.W.J. and Rogers, G.E. (1971). Biochemistry,10Google Scholar
  16. 16.
    Kornguth, M.L., Neidle, A., and Waelsch, H. (1963). Biochemistry, 2, 740.CrossRefPubMedGoogle Scholar
  17. 17.
    Benson, J.V. Jr., Gordon, M.J., and Patterson, J.A. (1967). Analyt. Biochem., 18, 228.CrossRefGoogle Scholar
  18. 18.
    Asquith, R.S., Otterburn, M.S., and Gardner, K.L. (1971). Experientia, 27, 1388.CrossRefGoogle Scholar
  19. Asquith, R.S., Otterburn, M.S., and Sinclair, W.J. (1974). Angew. Chem. Int. Ed. EngZ. ,13, 514.Google Scholar
  20. 20.
    Asquith, R.S. and Otterburn, M.S. (1971). AppZ. Polym. Symp., No. 18, 277.Google Scholar
  21. 21.
    Fasold, H., Klappenberger, J., Meyer, C., and Remold, H. (1971). Angew. Chem. Int. Ed. Engl., 10, 795.CrossRefPubMedGoogle Scholar
  22. 22.
    Wold, F. (1967). Methods Enzyrnol., 11, 617.CrossRefGoogle Scholar
  23. 23.
    Zahn, H. and Meienhofer, J. (1956). Melliand Textilber., 37, 432.Google Scholar
  24. 24.
    Zahn, H., Rouette, H.K., and Schade, F. (1965). “Int. Wool Text. Res. Conf, 3rd, Paris, 1965”, Sect. II, p. 495, Institut Textile de France, Paris.Google Scholar
  25. 25.
    Leach, S.J., Milligan, B., Inglis, A.S., Frazer, L.A., and Holt, L.A. (1968). “Symposium on Fibrous Proteins Australia 1967”, W.G. Crewther, ed., Butterworths ( Australia ), Sydney, p. 373.Google Scholar
  26. 26.
    Hille, E. (1962). Text.-Prax., 17, 171.Google Scholar
  27. 27.
    Asquith, R.S. and Chan, D.K. (1971). J. Soc. Dyers Colour., 87, 181.CrossRefGoogle Scholar
  28. 28.
    Caldwell, J.B. and Milligan, B. (1972). Text. Res. J., 42, 122.CrossRefGoogle Scholar
  29. 29.
    Bohak, Z. (1964). J. Biol. Chem., 239, 2878.PubMedGoogle Scholar
  30. 30.
    Horn, M.J., Jones, D.B., and Ringel, S.J. (1941). J. Biol. Chem., 138, 141.Google Scholar
  31. 31.
    Neuberger, A. (1948). Adv. Protein Chem., 4, 297.CrossRefPubMedGoogle Scholar
  32. 32.
    Nyilasi, J. (1957). Acta Chim. Acad. Sci. Hung., 10, 353.Google Scholar
  33. 33.
    Hill, J. and Leach, S.J. (1964). Biochemistry, 3, 1814.CrossRefPubMedGoogle Scholar
  34. 34.
    Provansal, M.M.P., Cuq, J.A., and Chef tel, J. (1975). J. Agric. Food Chem., 23, 938.CrossRefPubMedGoogle Scholar
  35. 35.
    Nomoto, M., Narahashi, Y., and Murakami, M. (1960). Biochem. J. (Japan), 48, 906.Google Scholar
  36. 36.
    Dutta, A.S. and Giles, M.B. (1976). J. Chem. Soc. Perkin Trans. 1, 244.CrossRefGoogle Scholar
  37. 37.
    Zahn, H., Kunitz, F.-W., and Hildebrand, D. (1960). J. Text. Inst., 51, T740.CrossRefGoogle Scholar
  38. 38.
    Holt, L.A. and Milligan, B. (1973). Aust. J. BioZ. Sci., 26, 871.Google Scholar

Copyright information

© Springer Science+Business Media New York 1977

Authors and Affiliations

  • Brian Milligan
    • 1
  • Leo A. Holt
    • 1
  1. 1.Division of Protein ChemistryC.S.I.R.O.ParkvilleAustralia

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