Genetic Analysis of the Soybean — Heterodera Glycines Interaction

  • Charles H. Opperman
  • Ke Dong
  • Stella Chang
Part of the NATO ASI Series book series (NSSA, volume 268)


An understanding of the genetics of nematode-plant interaction is essential to development of both classically and biotechnologically derived resistant host cultivars. Unfortunately, the study of this interaction has been very one-sided, focusing primarily on the genetics of plant resistance and almost not at all on the genetics of nematode parasitism. The small size and obligately parasitic life habit of phytophagous nematodes has hindered genetic analysis of nematode parasitism. In addition, many of the most important sedentary endoparasitic forms exhibit modified reproductive strategies (e.g., mitotic or meiotic parthenogenesis in Meloidogyne spp.) that preclude classical genetic approaches to analysis. The cyst nematodes, however, are primarily amphimictic and are amenable to genetic analyses.


Recombinant Inbred Line Nematode Parasitism Cyst Nematode Nematode Population Soybean Cyst Nematode 
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  1. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J., 1990, Basic local alignment search tool, J. Mol. Biol. 215:403.PubMedGoogle Scholar
  2. Alston, D.G., and Schmitt, D.P., 1988, Development of Heterodera glycines life stages as influenced by temperature, J. Nematol. 20:366.PubMedGoogle Scholar
  3. Anand, S.C., 1992, Registration of Hartwig soybean, Crop Sci. 32:1069.CrossRefGoogle Scholar
  4. Anand, S.C., and Brar, G.S., 1983, Response of soybean lines to differentially selected cultures of soybean cyst nematode Hete rodera glycines Ichinoe, J. Nematol. 15:120.PubMedGoogle Scholar
  5. Anand, S.C., and Gallo, K.M., 1984, Identification of additional soybean germplasm with resistance to race 3 of soybean cyst nematode, Plant Dis. 68:593.Google Scholar
  6. Anand, S.C., Myers, G.O., and Rao-Arelli, A.P., 1988, Resistance to race 3 of soybean cyst nematode in PI 437654, Crop Sci. 28:563.CrossRefGoogle Scholar
  7. Anand, S.C., and Rao-Arelli, A.P., 1989, Genetic analysis of soybean genotypes resistant to soybean cyst nematode race 5, Crop Sci. 29:1181.CrossRefGoogle Scholar
  8. Anand, S.C., and Shumway, C.R., 1985, Response of secondary selection on soybean cyst nematode reproduction on resistant soybean lines, Crop Prot. 4:231.CrossRefGoogle Scholar
  9. Andersen, S., 1965, Heredity of race 1 or race 2 in Heterodera avenae, Nematologica 11:121.CrossRefGoogle Scholar
  10. Andersen, S., and Andersen, K., 1982, Suggestions for determination and terminology of pathotypes and genes for resistance in cyst-forming nematodes, especially Heterodera avenae, OEPP/EPPO Bull. 12:379CrossRefGoogle Scholar
  11. Botstein, D., White, R.L., Skolnick, M.H., and Davis, R.W., 1980, Construction of a genetic map in man using restriction fragment length polymorphisms, Am. J. Hum. Genet. 32:314.PubMedGoogle Scholar
  12. Brim, C.A., and Ross, J.P., 1966, Registration of Pickett soybeans, Crop Sci. 6:305.CrossRefGoogle Scholar
  13. Burr, B., Evola, S.V., and Burr, F.A., 1983, The application of restriction fragment length polymorphism to plant breeding, in: “Genetic Engineering-Principles and Methods,” J.K. Setlow and Hollander, A., eds., Plenum, New York.Google Scholar
  14. Caldwell, B.E., Brim, CA., and Ross, J.P., 1960, Inheritance of resistance of soybeans to cyst nematode, Heterodera glycines, Agron. J. 52635.Google Scholar
  15. Caviness, C.E., 1992, Breeding for resistance to soybean cyst nematode, in: “Biology and Management of the Soybean Cyst Nematode,” R.D Riggs and Wrather, J. A., eds., APS Press, St. Paul, MN.Google Scholar
  16. Cook, R., and York, P.A., 1982, Resistance of cereals to Heterodera avenae: Methods of investigation, sources, and inheritance of resistance, OEPP/EPPO Bull 12:423.CrossRefGoogle Scholar
  17. Dropkin, V.H., and J.M. Halbrendt, 1986, Inbreeding and hybridizing soybean cyst nematodes on pruned soybeans in petri plates, J. Nematol. 18:200.PubMedGoogle Scholar
  18. Emmons, S., 1988, The genome, in: “The Nematode Caenorhabditis elegans,” W.B. Wood, ed., Cold Spring Harbor Laboratory, Cold Spring Harbor.Google Scholar
  19. Endo, B. Y., 1992, Cellular responses to infection, in: “Biology and Management of the Soybean Cyst Nematode,” R.D Riggs and Wrather, J. A., eds., APS Press, St. Paul, MN.Google Scholar
  20. Epps, J.M., and Hartwig, E.E., 1972, Reaction of soybean varieties and strains to race 4 of the soybean cyst nematode, J. Nematol. 4:222.Google Scholar
  21. Esbenshade, P.R., and Triantaphyllou, A.C., 1988, Genetic analysis of esterase polymorphism in the soybena cyst nematode Heterodera glycines, J. Nematoi. 20:486.Google Scholar
  22. Fire, A., 1986, Integrative transformation of Caenorhabditis elegans, EMBO J. 5:2673.PubMedGoogle Scholar
  23. Goldstein, P., and Triantaphyllou, A.C., 1979, Karyotype analysis of the plant-parasitic nematode Heterodera glycines by electron microscopy. II. The tetraploid and an aneuploid hybrid, J. Cell Sci. 43:225.Google Scholar
  24. Golden, A.M., Epps, J.M., Riggs, R.D., Duclos, L.A., Fox, J.A., and Bernard, R.L., 1970, Terminology and identity of infraspecific forms of the soybean cyst nematode (Heterodera glycines), Plant Dis. Rep. 54:544.Google Scholar
  25. Hancock, J. A., Hancock, F.G., Caviness, C.E., and Riggs, R.D., 1987, Genetics of resistance to “Race X” of soybean cyst nematode, Crop Sci. 27:704.CrossRefGoogle Scholar
  26. Hartwig, E.E., 1985, Breeding productive soybeans with resistance to soybean cyst nematode, in: “World Soybean Research Conference III,” R.A. Shibles, ed., Westview Press, Boulder.Google Scholar
  27. Hussey, R.S., 1989, Disease-inducing secretions of plant parasitic nematodes, Ann. Rev. Phytopath. 27:123.CrossRefGoogle Scholar
  28. Innes, M.A., Gelfand, D.H., Snitsky, J J., and White, T.J., 1990, “PCR Protocols,” Academic Press, San Diego.Google Scholar
  29. Janssen, R., Bakker, J., and Gommers, F.J., 1990, Selection of virulent and avirulent lines of Globodera rostochiensis for the H1 resistance gene in Solanum tuberosum ssp. andigena CPC 1673, Rev. Nematoi. 13:265.Google Scholar
  30. Janssen, R., Bakker, J., and Gommers, F.J., 1991, Mendelian proof for a gene-for-gene relationship between Globodera rostochiensis and the H1 resistance gene from Solanumtuberosum ssp. andigena CPC 1673, Rev. Nematoi. 14:213.Google Scholar
  31. Jones, M.G.K., 1981, The development and function of plant cells modified by endoparasitic nematodes, in: “Plant Parasitic Nematodes, Vol. 3,” B. M. Zuckerman and Rohde, R.A., eds., Academic Press, New York.Google Scholar
  32. Jones, M.G.K., and Dropkin, V.H., 1975, Scanning electron microscopy of syncytial transfer cells induced in roots by cyst-nematodes, Phys. Plant Path. 7:259.CrossRefGoogle Scholar
  33. Luedders, V.D., 1983, Genetics of the cyst nematode-soybean symbiosis, Phytopath. 73:944.CrossRefGoogle Scholar
  34. Luedders, V.D., 1985, Selection and inbreeding of Heterodera glycines on Glycine max, J. Nematoi. 17:400.Google Scholar
  35. Luedders, V.D., and Dropkin, V.D., 1983, Effects of secondary selection on cyst nematode reproduction on soybean, Crop Sci. 23:263.CrossRefGoogle Scholar
  36. Matson, A.L., and Williams, L.F., 1965, Evidence for a fourth gene for resistance to soybean cyst nematode, Crop Sci. 5:477.CrossRefGoogle Scholar
  37. McCann, J., Luedders, V.D., and Dropkin, V.D., 1982, Selection and reproduction of soybean cyst nematodes on resistant soybeans, Crop Sci. 22:78.CrossRefGoogle Scholar
  38. Miller, L.I., 1970, Differentiation of eleven isolates as races of the soybean cyst nematode. Phytopath. 60:1016Google Scholar
  39. Michelmore, R.W., Paran, I., and Kesseli, R.V., 1991, Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations, Proc. Nat. Acad. Sci. USA 88:9828.PubMedCrossRefGoogle Scholar
  40. Netscher, C., 1969, L’ovogénese et la reproduction chez Heterodera oryzae et H. sacchari (Nematodea: Heteroderidae), Nematologica 15:10.CrossRefGoogle Scholar
  41. Niblack, T. L., 1992, The race concept, in: “Biology and Management of the Soybean Cyst Nematode,” R.D Riggs and Wrather, J. A., eds., APS Press, St. Paul, MN.Google Scholar
  42. Noel, G. R., 1992, History, distribution, and economics, in: “Biology and Management of the Soybean Cyst Nematode,” R.D Riggs and Wrather, J. A., eds., APS Press, St. Paul, MN.Google Scholar
  43. Person, F., and Rivoal, R., 1979, Hybridation entre les races Frl et Fr4 d’Heterodera avenae Wollenweber en France et étude du comportement d’agressivite des descendants F1, Rev. Nematoi 2:177.Google Scholar
  44. Price, M., Caviness, C.E., and Riggs, R.D., 1978, Hybridization of races of Heterodera glycines, J. Nematol. 10:114.PubMedGoogle Scholar
  45. Rao-Arelli, A.P., and Anand, S.C., 1988, Genetic relationships among soybean plant introductions for resistance to race 3 of soybean cyst nematode, Crop Sci. 28:650.CrossRefGoogle Scholar
  46. Rao-Arelli, A.P., Anand, S.C., and Wrather, J.A., 1992, Soybean resistance to soybean cyst nematode race 3 is conditioned by an additional dominant gene, Crop Sci. 32:862.CrossRefGoogle Scholar
  47. Riggs, R.D., Hamblen, M.L., and Rakes, L., 1981, Infraspecific variation in reaction to hosts in Heterodera glycines populations, J. Nematol. 13:171.Google Scholar
  48. Riggs, R.D., and Schmitt, D.P., 1988, Complete characterization of the race scheme for Heterodera glycines, J. Nematol. 20:392.PubMedGoogle Scholar
  49. Rodrfguez-Kábana, R., 1992, “Chemical control,” in: “Biology and Management of the Soybean Cyst Nematode,” R.D Riggs and Wrather, J. A., eds., APS Press, St. Paul, MN.Google Scholar
  50. Ross, J.P., 1962, Physiological strains of Heterodera glycines, Plant Dis. Rep. 46:766.Google Scholar
  51. Ross, J.P. and Brim, C.A., 1957, Resistance of soybeans to the soybean cyst nematode as determined by the double row method, Plant Dis. Rep. 41:923.Google Scholar
  52. Sambrook, J. E., Fritsch, E. F. and Maniatis, T., 1989, “Molecular Cloning: A Laboratory Manual,” 2nd edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor.Google Scholar
  53. Sasser, J.N., and Freckman, D.W., 1987, “A world perspective on nematology: The role of the society,” in: “Vistas on Nematology,” J.A. Veech and Dickson, D.W., eds., Society of Nematologists, Inc., Hyattsville.Google Scholar
  54. Schmitt, D.P., and Noel, G.R., 1984, Nematode parasites of soybean, in: “Plant and Insect Nematodes,” W. R. Nickle, ed., Marcel Dekker, Inc., New York.Google Scholar
  55. Thomas, J.D., Caviness, C.E., Riggs, R.D., and Hartwig, E.E., 1975, Inheritance of reaction to race 4 of soybean cyst nematode, Crop Sci. 15:208.CrossRefGoogle Scholar
  56. Triantaphyllou, A.C., 1970, Oogenesis and reproduction of the birch cyst nematode, Heterodera betulae, J. Nematol. 2:399.PubMedGoogle Scholar
  57. Triantaphyllou, A.C., 1975, Genetic structure of races of Heterodera glycines and inheritance of ability to reproduce on resistant soybean, J. Nematol. 7:356.PubMedGoogle Scholar
  58. Triantaphyllou, A.C., 1982, Cyotgenetics and sexuality of root-knot and cyst nematodes. South. Coop. Ser. Bull. 276:71.Google Scholar
  59. Triantaphyllou, A.C., 1987, Genetics of nematode parasitism on plants, in: “Vistas on Nematology,” J.A. Veech and Dickson, D.W., eds., Society of Nematologists, Inc., Hyattsville.Google Scholar
  60. Triantaphyllou, A.C., and Hirschmann, H., 1962, Oogenesis and mode of reproduction in the soybean cyst nematode, Heterodera glycines, Nematologica 7:235.CrossRefGoogle Scholar
  61. Triantaphyllou, A.C., and Hirschmann, H., 1978, Cytology of the Heterodera trifolii pathenogenetic species complex, Nematologica 24:418.CrossRefGoogle Scholar
  62. Triantaphyllou, A.C., and Riggs, R.D., 1979, Polploidy in an amphimictic population of Heterodera glycines, J. Nematol. 11:371.PubMedGoogle Scholar
  63. Welsh, J., and McClelland, M., 1990, Fingerprinting genomes with PCR using arbitrary primers, Nuc. Acids Res. 18:7213.CrossRefGoogle Scholar
  64. Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rifalski, J.A., and Tingey, S.V., 1990, DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nuc. Acids Res. 18:6531.CrossRefGoogle Scholar
  65. Wyss, U., and Zunke, U., 1986, Observations on the behavior of second stage juveniles of Heterodera schachtii inside host roots, Rev. Nematol. 9:153.Google Scholar
  66. Wyss, U., and Zunke, U., 1992, Observations on the feeding behavior of Heterodera schachtii throughout development, including events during molting, Fund. appl. Nematol. 15:75–89.Google Scholar
  67. Young, L.D., 1982, Reproduction of differentially selected soybean cyst nematode populations on soybeans, Crop Sci. 22:385.CrossRefGoogle Scholar
  68. Young, L.D., 1984, Changes in the reproduction of Heterodera glycines on different lines of Glycine max, J. Nematol. 16:304.PubMedGoogle Scholar
  69. Young, L.D., 1992, Epiphytology and life cycle, in: “Biology and Management of the Soybean Cyst Nematode,” R.D Riggs and Wrather, J.A., eds., APS Press, St. Paul, MN.Google Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Charles H. Opperman
    • 1
    • 2
  • Ke Dong
    • 1
  • Stella Chang
    • 1
  1. 1.Department of Plant PathologyNorth Carolina State UniversityRaleighUSA
  2. 2.Department of GeneticsNorth Carolina State UniversityRaleighUSA

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