Molecular and Cellular Dissection of Giant Cell Function

  • David McK. Bird
  • Mark A. Wilson
Part of the NATO ASI Series book series (NSSA, volume 268)


Plant parasitic nematodes significantly affect the productive capability of the world’s farmlands. Sasser and Freckman (1987) have calculated that they reduce the yield of the world’s forty major food staples and cash crops by an average of 12.3%, with the losses being substantially higher for some commodities (e.g., 20.6% for tomato). The US proportion, in monetary terms, is in excess of $5.8 billion annually. As a group, the root-knot nematodes (Meloidogyne spp.) are the major contributors to these losses.


Giant Cell Cyst Nematode Nurse Cell Feeding Site Xylem Parenchyma 
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  1. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J., 1990, Basic local alignment search tool, J. Mol. Biol. 215:403.PubMedGoogle Scholar
  2. Bachmair, A., Becker, F., Masterson, R.V., and Schell, J., 1990, Perturbation of the ubiquitin system causes leaf curling, vascular tissue alterations and necrotic lesions in a higher plant, EMBO J. 9:4543.PubMedGoogle Scholar
  3. Belyavsky, A., Vinogradova, T., and Rajewsky, K., 1989, PCR-based cDNA library construction: general cDNA libraries at the level of a few cells, Nucl. Acids Res. 17:2919.PubMedCrossRefGoogle Scholar
  4. Bilofsky, H.S., and Burks, C., 1988, The GenBank (R) sequence data bank, Nucl. Acids Res. 16:1861.PubMedCrossRefGoogle Scholar
  5. Bird, A.F., 1961, The ultrastructure and histochemistry of a nematode-induced giant cell, J. Biophys. Biochem. Cytol. 11:701.PubMedCrossRefGoogle Scholar
  6. Bird, A.F., 1962, The inducement of giant cells by Meloidogyne javanica, Nematologica 8:1.CrossRefGoogle Scholar
  7. Bird, A.F., 1971, Quantitative studies on the growth of syncytia induced in plants by root knot nematodes, Int. J. Parasitol. 2:157.CrossRefGoogle Scholar
  8. Bird, D.McK., 1992, Mechanisms of the Meloidogyne -host interaction, in: “Nematology: from Molecule to Ecosystem,” F.J. Gommers, and Maas, P.W.Th., eds., ESN Inc., Dundee.Google Scholar
  9. Bird, D.McK, and Riddle, D.L., 1989, Molecular cloning and sequencing of ama-1, the gene encoding the largest subunit of Caenorhabditis elegans RNA polymerase II, Molec. Cell. Biol. 9:4119.PubMedGoogle Scholar
  10. Calabretta, B., and Nicolaides, N.C., 1992, C-myb and growth control. Crit. Rev. Euk. Gene Expression 2:225.Google Scholar
  11. Conkling, M.A., Cheng, C.-L., Yamamoto, Y.T., and Goodman, H.M., 1990, Isolation of transcriptionally regulated root-specific genes from tobacco, Plant Physiol. 93:1203.PubMedCrossRefGoogle Scholar
  12. Cornu, M., 1879, Etudes sur le Phylloxera vastatrix, Mém. Prés. Acad Sci. Paris. 26:164.Google Scholar
  13. Dietrich, M.A., Prenger, J.P., and Guillfoyle, T.J., 1990, Analysis of the genes encoding the largest subunit of RNA polymerase II in Arabidopsis and soybean, Plant Mol. Biol. 15:207.PubMedCrossRefGoogle Scholar
  14. Domec, C., Garbay, B., Fournier, M., and Bonnet, J., 1990, cDNA library construction from small amounts of unfractionated RNA: association of cDNA synthesis with polymerase chain reaction amplification, Anal. Biochem. 188:422.PubMedCrossRefGoogle Scholar
  15. Dorhout, R., Gommers, F.J., and Kollöffel, C., 1991, Water transport through tomato roots infected with Meloidogyne incognita. Phytopathology 81:379.CrossRefGoogle Scholar
  16. Dorhout, R., Kolloffel, C., and Gommers, F.J., 1992, Alteration of distribution of regions with high net proton extrusion in tomato roots infected with Meloidogyne incognita, Physiol. Molec. Plant Pathol. 40:153.CrossRefGoogle Scholar
  17. Duguid, J.R., Rowher, R.G., and Seed, B., 1988, Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridization of a cDNA library, Proc. Natl. Acad. Sci. USA 85:5738.PubMedCrossRefGoogle Scholar
  18. Endo, B.Y., 1987, Histopathology and ultrastructure of crops invaded by certain sedentary endoparasitic nematodes, in: “Vistas on Nematology,” J.A. Veech, and Dickson, D.W., eds., Society of Nematologists Inc., Hyattsville.Google Scholar
  19. Evans, I.M., Swinhoe, R., Gatehouse, L.N., Gatehouse, J.A., and Boulter, D., 1988, Distribution of root mRNA species in other vegatative organs of pea (Pisum sativum L.). Mol. Gen. Genet. 214:153.CrossRefGoogle Scholar
  20. Ewing, N.N., Wimmers, L.E., Meyer, D.J., Chetelat, R.T., and Bennett, A.B., 1990, Molecular cloning of tomato plasma membrane H+-ATPase, Plant Physiol. 94:1847.CrossRefGoogle Scholar
  21. Finley, D., and Chau, V., 1991, Ubiquitination, Annu. Rev. Cell Biol. 7:25.PubMedCrossRefGoogle Scholar
  22. Girod, P.-A., Carpenter, T.B., Nocker, S., Sullivan, M.L., and Vierstra, R.D., 1993, Homologs of the essential ubiquitin conjugating enzymes UBC1, 4, and 5 in yeast are encoded by a multigene family in Arabidopsis thaliana, Plant J. 3:545.Google Scholar
  23. Goddijn, O.J.M., Lindsey, K., van der Lee, F.M., Klap, J.C., and Sijmons, P.C., 1993, Differential gene expression in nematode-induced feeding structures of transgenic plants harbouring promoter-gusA fusion constructs, Plant Cell 4:863.Google Scholar
  24. Grandbastien, M.A., Spielmann, A., and Caboche, M., 1989, Tnt-1, a mobile retroviral-like transposable element of tobacco, isolated by plant genetics, Nature 337:376PubMedCrossRefGoogle Scholar
  25. Grundler, F.M.W., Böckenhoff, A., Schmidt, K-P., Sobczak, M., Golinowski, W., and Wyss, U., 1994, Arabidopsis thaliana and Heterodera schachtii: a versatile model to characterize the interaction between host plants and cyst nematodes, in: “NATO ARW: Advances in Molecular Plant Nematology,” F. Lamberti, De Giorgi, C., and Bird, D.McK., eds., Plenum Press, New York.Google Scholar
  26. Gurr, S.J., McPherson, M.J., Scollan, C., Atkinson, H.J., and Bowles, D.J., 1991, Gene expression in nematode-infected plant roots. Mol. Gen. Genet. 226:361.PubMedCrossRefGoogle Scholar
  27. Hatfield, P.M., and Vierstra, R.D., 1992, Multiple forms of ubiquitin-activating enzyme E1 from wheat, J. Biol. Chem. 267:14799.PubMedGoogle Scholar
  28. Hershko, A., and Ciechanover, A., 1992, The ubiquitin system for protein degradation, Annu. Rev. Biochem. 61:76.CrossRefGoogle Scholar
  29. Hussey, R.S., 1989, Disease-inducing secretions of plant-parasitic nematodes, Annu. Rev. Phytopathol. 27:123.CrossRefGoogle Scholar
  30. Hussey, R.S., Davis, E.L., and Ray, C., 1994, Meloidogyne stylet secretions, in: “NATO ARW: Advances in Molecular Plant Nematology,” F. Lamberti, De Giorgi, C., and Bird, D.McK., eds., Plenum Press, New York.Google Scholar
  31. Hussey, R.S., Mims, C.W., and Westcott, S.W., 1992a. Ultrastructure of root cortical cells parasitized by the ring nematode Criconemella xenoplax, Protoplasma 167:55.CrossRefGoogle Scholar
  32. Hussey, R.S., Mims, C.W., and Westcott, S.W., 1992b. Immunocytochemical localization of callose in root cortical cells parasitized by the ring nematode Criconemella xenoplax, Protoplasma 171:1.CrossRefGoogle Scholar
  33. Jones, M.G.K., and Northcote, D.H., 1972, Nematode-induced giant-cell formation in roots of Impatiens balsamina, J. Nematol. 10:70.Google Scholar
  34. Jones, M.G.K., Novacky, A., and Dropkin, V.H., 1975, Transmembrane potentials of parenchyma cells and nematode-induced transfer cells, Protoplasma 85:15.CrossRefGoogle Scholar
  35. Jones, M.G.K., and Payne, H.L., 1978, Early stages of nematode-induced syncytium — a multinucleate transfer cell, J. Cell Set 10:789.Google Scholar
  36. Kiss, T., Kiss, M., and Solomosy, F., 1989, Nucleotide sequence of a 25S rRNA gene from tomato, Nucl. Acids Res. 17:796.PubMedCrossRefGoogle Scholar
  37. Krusberg, L.R., and Nielsen, L.W., 1958, Pathogenesis of root-knot nematodes to the Porto (sic) Rico variety of sweetpotato, Phytopathology 48:30.Google Scholar
  38. Lebeau, M., Alvarez-Bolado, G., Wahli, W., and Catsicas, S., 1991, PCR driven DNA-DNA competitive hybridization: a new method for sensitive differential cloning, Nucl. Acids Res. 19:4778.PubMedCrossRefGoogle Scholar
  39. Magnusson, C., and Golinowski, W., 1991, Ultrastructural relationships of the developing syncytium induced by Heterodera schachtii (Nematoda) in root tissue of rape, Can. J. Bot. 69:44.CrossRefGoogle Scholar
  40. Mangiarotti, G., Chung, S., Zuker, C., and Lodish, H.F., 1981, Selection and analysis of cloned developmentally-regulated Dictyostelium discoidium genes by hybridization-competition. Nucl. Acids Res. 9:947.PubMedCrossRefGoogle Scholar
  41. Mankau, R., and Linford, M.B., 1960, Host-parasite relationships of the clover cyst nematode Heterodera trifolii Goffart. Bulletin 667, University of Illinois, AES.Google Scholar
  42. Marks, M.D., Esch, J., Herman, P., Sivakumaran, S., and Oppenheimer, D., 1991, A model for cell-type determination and differentiation in plants, Symp. Soc. Expt. Biol. 45:77.Google Scholar
  43. Marocco, A., Wissenbach, M., Becker, D., Paz-Ares, J., Saedler, H., Salaminin, F., and Rohde, W., 1989, Multiple genes are transcribed in Hordeum vulgare and Zea mays that carry the DNA binding domain of the myb oncoproteins, Molec. Gen. Genet. 216:183.PubMedCrossRefGoogle Scholar
  44. Moriau, L., Bogaerts, P., Jonniaux, J.-L., and Boutry, M., 1993, Identification and characterization of a second plasma membrane H+-ATPase gene subfamily in Nicotiana plumbagininiflora, Plant Molec. Biol. 21:955.CrossRefGoogle Scholar
  45. Mundo-Ocampo, M., Greene, M., Flaxman, M., and Baldwin, J.G., 1993, Morphology of nurse cell nuclei induced by Meloidodera floridensis: A computer graphics application, J. Nematol. 25:603.PubMedGoogle Scholar
  46. Nagl, W., 1978, “Endopolyploidy and Polytene in Differentiation and Evolution,” Elsevier/North Holland Biomedical, Amsterdam.Google Scholar
  47. Niebel, A., de Almeida, J., Van Montagu, M., and Gheysen, G., 1993a, Identification of root knot nematode-resistant mutants in Arabidopsis thaliana, in: “Mechanisms of Plant Defense Responses,” B. Fritig, and Legrand, M. eds., Kluwer Academic Publishers, Dordrecht.Google Scholar
  48. Niebel, A., Barthels, N., de Almeida-Engler, J., Karimi, M., Vercauteren, I., Van Montagu, M, and Gheysen, G., 1994, Arabidopsis thaliana as a model host plant to study molecular interactions with root-knot and cyst nematodes, in: “NATO ARW: Advances in Molecular Plant Nematology,” F. Lamberti, De Giorgi, C., and Bird, D.McK., eds., Plenum Press, New York.Google Scholar
  49. Niebel, A., Engler, J.deA., Tiré, C., Engler, C., Van Montagu, M., and Gheysen, G., 1993b, Induction patterns of an extensin gene in tobacco upon nematode infection, Plant Cell 5:1697.PubMedGoogle Scholar
  50. Niebel, A., Van der Eycken, W., Van Montagu, M., and Gheysen, G., 1993c, Isolation and characterization of two potato genes induced during cyst nematode infection, Arch. Int. Physiol. Biophys. 101:B18.Google Scholar
  51. O’Bannon, J.H., and Reynolds, H.W., 1965, Water consumption and growth of root-knotnematode-infected and uninfected cotton plants, Soil Sci. 99:251.CrossRefGoogle Scholar
  52. Opperman, C.H., Taylor, C.G., and Conkling, M.A., 1994, Root-knot nematode-directed expression of a plant root-specific gene, Science 263:221.PubMedCrossRefGoogle Scholar
  53. Press, M.C., and Whittaker, J.B., 1993, Exploitation of the xylem stream by parasitic organisms, Phil. Trans. R. Soc. Lond. B 341:101.CrossRefGoogle Scholar
  54. Rubenstein, J.L.R., Brice, A.E.J., Ciaranello, R.D., Denny, D., Porteus, M.H., and Usdin, T.B., 1990, Subtractive hybridization system using single-stranded phagemids with directional inserts, Nucl. Acids Res. 18:4833.PubMedCrossRefGoogle Scholar
  55. Sargent, T.D., and Dawid, I.B., 1985, Differential gene expression in the gastrula of Xenopus laevis, Science 222:135.CrossRefGoogle Scholar
  56. Sasser, J.N. and Freckman, D.W., 1987, A world perspective on nematology: the role of the society, in: “Vistas on Nematology,” J.A. Veech, and Dickson, D.W., eds., Society of Nematologists Inc., Hyattsville.Google Scholar
  57. Serrano, R., 1989, Structure and function of plasma membrane ATPase, Ann. Rev. Plant Physiol. 40:61.CrossRefGoogle Scholar
  58. Seufert, W., McGrath, J.P., and Jentsch, S., 1990, UBC1 encodes a novel member of an essential subfamily of yeast ubiquitin-conjugating enzymes involved in protein degradation, EMBO J. 9:4535.PubMedGoogle Scholar
  59. Steeves, T.A., and Sussex, I.M., 1989, “Patterns in Plant Development,” Cambridge University Press, Melbourne.CrossRefGoogle Scholar
  60. Sullivan, M.L., and Vierstra, R.D., 1991, Cloning of a 16-kDa ubiquitin carrier protein from wheat and Arabidopsis thaliana, J. Biol. Chem. 266:2387.Google Scholar
  61. Sumimoto, H., Ohkuma, Y., Sinn, E., Kato, H., Shimasaki, S., Horikoshi, M., and Roeder, R.G., 1991, Conserved sequence motifs in the small subunit of human general transcription factor TFIIE, Nature 354:401.PubMedCrossRefGoogle Scholar
  62. Swaroop, A., Xu, J., Agarwal, N., and Weissman, S.M., 1991, A simple and efficient cDNA library subtraction procedure: isolation of human retina-specific cDNA clones, Nucl. Acids Res. 19:1954.PubMedCrossRefGoogle Scholar
  63. Visser, J., and Dörr, I., 1987, The haustorium, in: “Parasitic Weeds in Agriculture Vol. I: Striga,” L.J. Musselman, ed., CRC Press, Boca Raton.Google Scholar
  64. Voytas, D.F., and Ausubel, F.M., 1989, A copia-like element family in Arabidopsis thaliana, Nature 336:242.CrossRefGoogle Scholar
  65. Wasylyk, B., Hahn, S.L., and Giovane, A., 1993, The Ets family of transcription factors, Eur. J. Biochem. 21:7.CrossRefGoogle Scholar
  66. Welsh, J., Liu, J-P.X., and Efstatiadis, A., 1990, Cloning of PCR-amplified total cDNA: construction of a mouse oocyte cDNA library, Genet. Anal. Techn. Appl. 7:5.CrossRefGoogle Scholar
  67. Wilcox, D.A., and Loria R., 1986, Water relations, growth, and yield in two snap bean cultivars infected with root-knot nematode, Meloidogyne hapla (Chitwood), J. Am. Soc. Hortic. Sci. 11:34.Google Scholar
  68. Wilson, M.A., Bird, D.McK., and van der Knaap, E., 1994, A comprehensive subtractive cDNA cloning approach to identify nematode-induced transcripts in tomato, Phytopathology in press.Google Scholar
  69. Wyss U., Grundler, F.M.W., and Münch, A., 1992, The parasitic behaviour of 2nd-stage juveniles of Meloidogyne incognita in roots of Arabidopsis thaliana, Nematologica 38:98.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • David McK. Bird
    • 1
  • Mark A. Wilson
    • 1
  1. 1.Department of Nematology and Graduate Genetic GroupUniversity of California-RiversideRiversideUSA

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