Immunohistochemical Demonstration of Lactoferrin in Human Neoplastic Tissues
By immunohistochemistry, the distribution of lactoferrin (Lf) as well as other iron-binding proteins has been extensively investigated in normal human tissues such as stomach, kidney, lung, pancreas, liver and bone marrow1. In addition, Lf has been localized in epithelial elements of major salivary glands2 as well as in malignant counterparts3. Moreover, the present of Lf in prostatic tissue has been documented by radial-immunodiffusion in homogenates of prostatic cancer and benign hypertrophy4. In lactating mammary glands as well as in breast carcinomatous tissues, immunohistochemical studies stating the appearance of Lf are conflicting, with a variation in the occurence rate of stained elements1,5,6; however, no relationships have been found between Lf and other prognostic factors such as steroid receptors, histologic type and tumour grade.
KeywordsPapillary Carcinoma Intestinal Metaplasia Gallbladder Carcinoma Follicular Adenoma Epithelial Element
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- 2.Korsrud F.R. and Brandtzaeg P. (1982). Characterization of epithelial elements in human major salivary glands by functional markers: localization of amylase, lactoferrin, lysozyme, secretory component, and secretory immunoglobuline by paired immunofluorescence staining. J. Histochem. Cytochem. 30, 657–666.PubMedCrossRefGoogle Scholar
- 3.Caselitz J., Jaup T., and Seifert G. (1981). Lactoferrin and lysozyme in carcinomas of the parotid gland. Virchows Arch. ( A) 394, 61–73.Google Scholar
- 4.Van Camp K, Van Sande M. (1980). Vrije amino-zuren en ninhydrine positieve substanties in menselijk prostaatweefsel. Verh. K. Acad. Geneeskd. Belg. 42, 104.Google Scholar
- 5.Rossiello R., Carriero M.V. and Giordano G.G. (1984). Distribution of ferritin, transferrin and lactoferrin in breast carcinoma tissue. J. Clin. Pathol. 37, 51–55.Google Scholar
- 8.Tuccari G. and Barresi G. (1985). immunohistichemical demonstration of lactoferrin in follicular adenomas and thyroid carcinomas. Virchows Arch. (A) 406, 67–74.Google Scholar
- 10.Tuccari G., Barresi G., Arena F. and Inferrera C. (1989). lmmunocytochemical detection of lactoferrin in human gastric carcinomas and adenomas. Arch. Pathol. Lab. Med. 113, 912–916.Google Scholar
- 11.Tuccari G., Rizzo A., Crisafulli C. and Barresi G. (1992). Iron-binding proteins in human coloractal adenomas and carcinomas: an immunocytochemical investigation. Histol. Histopathol. 7, 543–547.Google Scholar
- 12.Tuccari G., Rossiello R., Barresi G. (1997). Iron binding proteins in gallbladder carcinomas. An immunocytochemical investigation. Histol. Histopathol. 12, (in press).Google Scholar
- 13.Weir E.E., Pretlow T.G. and Pitts A. (1974). A more sensitive and specific histochemical peroxidase for the localization of cellular antigen by the enzyme-antibody conjugate method. J. Histochem. Cytochem. 22, 1135–1140.Google Scholar
- 15.Tedeschi A., Tuccari G., Magazzù G., Arena F., Ricciardi R., Barresi G. (1987). Immunohistochemical localization of lactoferrin in duodenojejunal mucosa from celiac children. J. Pediatr. Gastroenterol. Nutr. 6, 328–334.Google Scholar
- 16.Cabaret V., Vilain M.O., Delobelle-Deroide A., Vanseymortier L. (1992). Détection immunohistochmique de la céruloplasmine et de la lactoferrine sur une série de 59 tumeurs thyroidiennes. Ann. Pathol. 12, 347–352.Google Scholar
- 22.Roiron D., Amouric M., Marvaldi J. and Figarella C. (1989). Lactoferrin-binding sites at the surface of HT29–D4 cells. Eur. J. Biochem. 186, 367–373.Google Scholar