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Advances in Lactoferrin Research pp 273–284Cite as

Inhibition of Azoxymethane Initiated Colon Tumor and Aberrant Crypt Foci Development by Bovine Lactoferrin Administration in F344 Rats

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 443))

Summary

The influence of bovine lactoferrin (bLf) on colon carcinogenesis was investigated in male F344 rats treated with azoxymethane (AOM). In experiment I, 2% and 0.2% bLf, and Bifidobacterium longum (B. longum) as a positive control at 3% were given in the diet for 4 weeks, along with two s.c. 15 mg/kg injections of AOM on days 1 and 8. The numbers of aberrant crypt foci (ACF) were decreased by both treatments. Similar results were obtained in experiment II of 13 weeks duration. In experiment III, animals were given three weekly injections of AOM and then received 2 or 0.2% bLf, 2% bLf-hydrolysate, or 0.1% bovine lactoferricin (bLfcin) for 36 weeks. No effects indicative of toxicity were noted, but significant reduction in both the incidence and number of adenocarcinomas of the large intestine was observed with almost all the treatments. Thus, the incidences of colon adenocarcinomas in the groups receiving 2 or 0.2% bLf, 2% bLf-hydrolysate, or 0.1% bLfcin were 15%, 25%, 26.3% and only 10%, respectively, in contrast to the 57.5% control value (p < 0.01). ACF values also exhibited reduced development. Investigation of beta-glucuronidase revealed decrease in the cecal contents of animals receiving bLf. In addition, demonstration of enhancement of NK activity by bLf indicated that its inhibitory effects could have been related to elevated immune cytotoxicity.

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Authors and Affiliations

  1. Chemotherapy Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-ku, Tokyo 104, Japan

    Hiroyuki Tsuda, Kazunori Sekine, Joe Nakamura, Yoshihiko Ushida, Tetsuya Kuhara, Nobuo Takasuka, Dae Joong Kim, Makoto Asamoto, Hiroyasu Baba-Toriyama & Malcolm A. Moore

  2. Department of Biochemistry, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602, Japan

    Hoyoku Nishino

  3. National Cancer Center Central Hospital, Tsukiji 5-1-1, Chuo-ku, Tokyo 104, Japan

    Tadao Kakizoe

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  1. Hiroyuki Tsuda
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Editor information

Editors and Affiliations

  1. Université des Sciences et Technologies de Lille, Villeneuve d’Ascq, France

    Geneviève Spik , Dominique Legrand , Joël Mazurier  & Annick Pierce , ,  & 

  2. Biopole, Brussels, Belgium

    Jean-Paul Perraudin

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© 1998 Springer Science+Business Media New York

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Tsuda, H. et al. (1998). Inhibition of Azoxymethane Initiated Colon Tumor and Aberrant Crypt Foci Development by Bovine Lactoferrin Administration in F344 Rats. In: Spik, G., Legrand, D., Mazurier, J., Pierce, A., Perraudin, JP. (eds) Advances in Lactoferrin Research. Advances in Experimental Medicine and Biology, vol 443. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9068-9_34

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  • DOI: https://doi.org/10.1007/978-1-4757-9068-9_34

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-9070-2

  • Online ISBN: 978-1-4757-9068-9

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