Interaction of Lactoferrin with Micrococcus spp. and Its Role in Antimicrobial Activity

  • A. de Lillo
  • R. Cernuda
  • J. H. Brock
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 443)


The host non-specific defence mechanisms are of major importance in those areas first encountered by invading microorganisms and influence the initial success of bacterial colonization of the host, especially in mucosal areas. Secretions bathing the mucosal surfaces contain several non-specific antibacterial components such as lysozyme, lactoperoxidase and lactoferrin (McNab and Tomasi, 1981) and specific ones such as secretory immunoglobulin A (sIgA) that control the growth of pathogens or commensal microbiota on mucosal surfaces.


Mucosal Surface Hydrophobic Interaction Chromatography Micrococcus Luteus Secretory Immunoglobulin Human Lactoferrin 
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  1. 1.
    Arnold, R.R., Russell, J.E., Champion, W.J., Brewer. M. and Gauthier, J.J. 1982 Bactericidal activity of human lactoferrin: differentiation from the stasis of iron deprivation. lnfect. lmmun. 35: 792–799.Google Scholar
  2. 2.
    Bellamy, W., Takase, M., Yamauchi, K. Wakabayashi, H., Kawase, K. and Tornita M. 1992. Identification of the bactericidal domain of lactoferrin. Biochim Bipophys Acta 1121: 130–136.CrossRefGoogle Scholar
  3. 3.
    Dalmastri, C., Valenti, P., Visca, P., Vittorioso. P. and Orsi N. 1988. Enhanced antimicrobial activity of lactoferrin by binding to the bacterial surface. Microbiologica 11: 225–230.PubMedGoogle Scholar
  4. 4.
    Ellison, R.T. III, LaForce, F.M., Giehl, T.J., Boose, D.S. and Dunn B.E. 1990. Lactoferrin and transferrin damage of the Gram-negative outer membrane is modulated by Ca “and Mg”.J. Gen. Microbiol. 136: 1437–1446.PubMedCrossRefGoogle Scholar
  5. 5.
    Fischer, W. 1991. One step purification of bacterial lipid macroamphiphiles by hydrophobic interaction chromatography. Anal. Biochem. 194: 353–358.PubMedCrossRefGoogle Scholar
  6. 6.
    Greenwood, F.C., Hunter W.M. and Glover, J.S. 1963. The preparation of’’’ I-labelled human growth hormone of high specific radioactivity. Biochem. J. 89: 114–123.PubMedGoogle Scholar
  7. 7.
    McNab, P.C. and Tomasi, T.B. 1981. Host defence mechanisms at mucosal surfaces. Annu. Rev. Microbiol. 35: 477.CrossRefGoogle Scholar
  8. 8.
    Naidu, S. and Arnold, R.R. 1997. Influence of lactoferrin on host-microbe interactions. In Lactoferrin. Interactions and Biological Functions. Ed T.W. Hutchens and B. Lönnerdal. Humana Press. New Jersey pp. 259–276.Google Scholar
  9. 9.
    Ohno, N. and Morrison D.C. 1989. Lipopolysaccharide interaction with lysozyme. J. Biol. Chem. 264: 4434–4441.PubMedGoogle Scholar
  10. 10.
    Powell, D.A., Duckworth, M. and Baddiley J. 1975. A membrane-associated lipomannan in Micrococci. Biochem J. 151: 387–397PubMedGoogle Scholar
  11. 11.
    Reiter, B. 1983. The biological significance of lactoferrin. Int. J. Tiss. Reac. 5: 87–96Google Scholar
  12. 12.
    Saito, H., Miyakawa, H., Tamura, Y., Shimamura, S. and Tornita, M. 1991. Potent bactericidal activity of bovine lactoferrin hydrolysate produced by heat treatment at acidic pH. J. Dairy Sci 74: 3724–3730.PubMedCrossRefGoogle Scholar
  13. 13.
    Tomita, M., W. Bellamy, M. Takase, K. Yamamuchi, H. Wakabayashi and Kawase, K. 1991. Potent antibacterial peptides generated by pepsin digestion of lactoferrin. J. Dairy Sci. 74: 4137–4142.PubMedCrossRefGoogle Scholar
  14. 14.
    Westphal, O. and Jann K. 1965. Bacterial lipopolysaccharides. Methods Carbohydr. Chem. 5: 83–91.Google Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • A. de Lillo
    • 1
    • 2
  • R. Cernuda
    • 3
  • J. H. Brock
    • 2
  1. 1.Department of Functional Biology (Microbiology) Medical SchoolUniversity of OviedoSpain
  2. 2.Department of ImmunologyWestern InfirmaryGlasgowScotland, UK
  3. 3.Department of Cellular Biology and Morphology Medical SchoolUniversity of OviedoSpain

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