Evidence for the Existence of a Surface Receptor(s) for Ferriclactoferrin and Ferrictransferrin Associated with the Plasma Membrane of the Protozoan Parasite Leishmania donovani

  • Bradley E. Britigan
  • Troy S. Lewis
  • Michael L. McCormick
  • Mary E. Wilson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 443)


Previous work has demonstrated the ability of the promastigote form of the protozoan parasite Leishmania chagasi to utilize iron chelated to lactoferrin and transferrin for growth and metabolism. We have obtained evidence suggesting that the promastigote form of the parasite possesses specific binding sites for lactoferrin and transferrin. Lactoferrin binding appears to be: 1) independent of whether or not the protein contains iron; 2) not inhibited by transferrin: and 3) independent of whether the organism is in log or stationary phase of growth. Transferrin binding is: 1) markedly greater if the protein is iron loaded; 2) inhibited by the presence of lactoferrin; and 3) independent of whether the organism is in log or stationary growth phase. Preliminary ligand blot analyses are consistent with the presence of a protein or proteins which bind lactoferrin and/or transferrin. The relationship to these binding sites to those described in other protozoan species requires further investigation.


Visceral Leishmaniasis Protozoan Parasite Trypanosoma Brucei Amastigote Form Promastigote Form 
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  1. 1.
    Wilson ME. Leishmaniasis. Curr Opin Infect Dis 1993; 6: 331–341.Google Scholar
  2. 2.
    Chang K-P, Hendricks LD. Laboratory cultivation and maintenance of Leishmania. In: Chang K-P, Bray RS. eds. Leishmaniasis. New York: Elsevier, 1985: 214–244.Google Scholar
  3. 3.
    Wilson ME, Vorhies RW, Andersen KA, Britigan BE. Acquisition of iron from transferrin and lactoferrin by the protozoan Leishmania chagasi. Infect Immun 1994; 62: 3262–3269.PubMedGoogle Scholar
  4. 4.
    Soteriadou K, Papavassiliou P, Voyiatzaki C, Boelaert J. Effect of iron chelation on the in-vitro growth of Leishmania promastigotes. J Antimicrob Chemother 1995; 35: 23–29.PubMedCrossRefGoogle Scholar
  5. 5.
    Voyiatzaki CS, Soteriadou KP. Identification and isolation of the Leishmania transferrin receptor. J Biol Chem 1992; 267: 9112–9117.PubMedGoogle Scholar
  6. 6.
    Voyiatzaki CS, Soteriadou KP. Evidence of transferrin binding sites on the surface of Leishmania promastigotes. J Biol Chem 1990; 265: 22380–22385.PubMedGoogle Scholar
  7. 7.
    McCormick ML, Wilson ME, Lewis TS, Vorhies RW, Britigan BE. Specific binding of ferrilactoferrin and ferritransferrin in the protozoan Leishmania chagasi. In: Hutchens TW, Lönnerdal B. eds. Lactoferrin: Interactions and Biological Functions. Totowa, NJ: Humana Press Inc. 1997: 333–342.Google Scholar
  8. 8.
    Steverding D, Stierhof YD, Fuchs H, Tauber R, Overath P. Transferrin-binding protein complex is the receptor for transferrin uptake in Trypanosoma brucei. J Cell Biol 1995; 131: 1173–1182.PubMedCrossRefGoogle Scholar
  9. 9.
    Ligtenberg MJL, Bitter W, Kieft R, Steverding D, Janssen H, et al. Reconstitution of a surface transferrin binding complex in insect form Trypanosoma brucei. EMBO J 1994; 13: 2565–2573.PubMedGoogle Scholar
  10. 10.
    Steverding D, Stierhof Y-D, Chaudhri M, Ligtenberg M, Schell D, et al. ESAG 6 and 7 products of Trvpanosoma brucei form a transferrin binding protein complex. Eur J Cell Biol 1994; 64: 78–87.PubMedGoogle Scholar
  11. 11.
    Maier A, Steverding D. Low affinity of Trypanosoma brucei transferrin receptor to apotransferrin at pH 5 explains the fate of the ligand during endocytosis. FEBS Lett 1996; 396: 87–89.PubMedCrossRefGoogle Scholar
  12. 12.
    Peterson KM, Alderete JF. Iron uptake and increased intracellular enzyme activity follow host lactoferrin binding by Trichomonas vaginalis receptors. J Exp Med 1984; 160: 398–410.PubMedCrossRefGoogle Scholar
  13. 13.
    Cornelissen CN, Sparling PF. Iron piracy: Acquisition of transferrin-bound iron by bacterial pathogens. Mol Microbiol 1994; 14: 843–850.PubMedCrossRefGoogle Scholar
  14. 14.
    Schryvers AB, Lee BC. Analysis of bacterial receptors for host iron-binding proteins. J Microbiol Methods 1993; 18: 255–266.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Bradley E. Britigan
    • 1
    • 2
  • Troy S. Lewis
    • 1
  • Michael L. McCormick
    • 1
    • 2
  • Mary E. Wilson
    • 1
    • 2
    • 3
    • 4
  1. 1.Research ServiceVA Medical Center-Iowa CityIowa CityUSA
  2. 2.Department of Internal MedicineUniversity of Iowa, College of MedicineIowa CityUSA
  3. 3.Department of MicrobiologyUniversity of Iowa, College of MedicineIowa CityUSA
  4. 4.Program in ImmunologyUniversity of Iowa, College of MedicineIowa CityUSA

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