Advertisement

Expression of Human Lactoferrin in Transfected Rat Mammary Epithelial Cells

  • Haruto Kumura
  • Yoriko Hiramatsu
  • Yukako Ukai
  • Katsuhiko Mikawa
  • Kei-ich Shimazaki
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 443)

Abstract

The expression of heterogeneous proteins in mammary gland of transgenic animals is an alternative method of producing recombinant proteins. Several groups have already described initial experiments in different animal systems, mainly in the mouse model system2. Although some works reported high expression level with an estimation by electrophoresis, adaptation of protein purification procedure is quite difficult in the mouse model system due to limited availability of mouse milk.

Keywords

Mammary Gland Human Lactoferrin Casein Gene Mouse Model System Mouse Milk 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Chen C, Okayama H (1987) Mol. Cell. Biol. 7, 2745–2752Google Scholar
  2. 2.
    Houdebine LH (1994) J. Biotechnol. 34, 269–287.PubMedCrossRefGoogle Scholar
  3. 3.
    Huynh HT, Robitaille G, Turner JD (1991) Exp. Cell. Res. 197, 191–199.Google Scholar
  4. 4.
    Kaetzel CS, Ray DB (1984) J. Dairy Sci. 67, 64–75.PubMedCrossRefGoogle Scholar
  5. 5.
    Kim Si, Cho YY, Lee KW, Yu DY, Lee CS, Han YM, Lee KK (1994) Mol. Cells 4, 355–360.Google Scholar
  6. 6.
    Kim SJ, Yu DY, Lee KW, Cho YY, Lee CS, Han YM, Lee KK (1995)1. Biochem. Mol. Biol. 28, 57–61.Google Scholar
  7. 7.
    Richards J, Larson L, Yang J, Guzman R, Tomooka Y, Osborn R, lmagawa W, Nandi S (1983) J. Tissue Cult. Meth. 8, 31–36.Google Scholar
  8. 8.
    Rijnkels M, Kooiman PM, Krimpenfort PJA, De Boer HA, Pieper FR (1995) Biochem. J. 311, 929–937.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Haruto Kumura
    • 1
  • Yoriko Hiramatsu
    • 1
  • Yukako Ukai
    • 1
  • Katsuhiko Mikawa
    • 1
  • Kei-ich Shimazaki
    • 1
  1. 1.Laboratory of Dairy Science Faculty of AgricultureHokkaido University060 SapporoJapan

Personalised recommendations