Growth Medium Considerations for the Scale-Up of S-Layer Protein Production by Bacillus brevis 47

  • Gordon K. Whitney
  • A. Wong
  • A. J. Daugulis
  • B. N. White
Chapter
Part of the NATO ASI Series book series (NSSA, volume 252)

Abstract

Bacillus brevis 47 is known to secrete 16 g/L of S-layer proteins (Wight et al., 1992) and therefore has great potential for the production of high levels of secreted heterologous proteins of commercial value. Investigations into the nutritional regulation of S-layer protein production should facilitate the production of foreign proteins whose genes are under the control of S-layer promoters. Polypeptone has been identified as the key nutrient for the stimulation of S-layer secretion (Shaku et al., 1980). Given the heterogeneous nature of this nutrient it should be possible to employ less expensive nitrogen sources, given medium cost considerations when scaling-up a commercial process.

Keywords

Yeast Extract Nitrogen Source Foreign Protein Commercial Process Sole Nitrogen Source 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Bradford, M., 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72: 248.PubMedCrossRefGoogle Scholar
  2. Shaku, M., Koike, S., and Udaka, S., 1980, Cultural conditions for protein production by Bacillus brevis no. 47, Agricult. Biol. Chem. 44: 99.CrossRefGoogle Scholar
  3. Wight, C.P., Whitney, G.K., White, B.N., and Daugulis, A.J., 1992, Enhancement and regulation of extracellular protein production by Bacillus brevis 47 through manipulation of cell culture conditions, Biotechnol. Bioeng. 40: 46.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Gordon K. Whitney
    • 1
  • A. Wong
    • 1
  • A. J. Daugulis
    • 1
  • B. N. White
    • 2
  1. 1.Department of Chemical EngineeringQueen’s University KingstonOntarioCanada
  2. 2.Biology DepartmentMcMaster UniversityHamiltonCanada

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