Skip to main content

Attachment of the S-Layer of Caulobacter crescentus to the Cell Surface

  • Chapter
Advances in Bacterial Paracrystalline Surface Layers

Part of the book series: NATO ASI Series ((NSSA,volume 252))

Abstract

In most cases S-layers attach to the outer membrane of gram-negative bacteria by non-covalent interactions (Koval and Murray, 1984; Sleytr and Messner, 1988). Lipopolysaccharide (LPS) (Dooley et al., 1985) or proteins (Thorne et al., 1975) have been implicated as the molecules to which the S-layer protein interacts to maintain cell association. This report suggests that another outer membrane component, termed the S-layer-associated oligosaccharide (SAO), is the molecule responsible for the attachment of RsaA, the S-layer protein of Caulobacter crescentus, to the cell surface.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Beveridge, T.J., and Murray, R.G.E., 1976, Dependence of the superficial layers of Spirillum putridiconchylium on Cat+ or Sr2+, Can. J. Microbiol. 22: 1233.

    Article  PubMed  CAS  Google Scholar 

  • Dooley, J.S.G., Lallier, R., Shaw, D.H., and Trust, T.J., 1985, Electrophoretic and immunochemical analyses of the lipopolysaccharides of various strains of Aeromonas hydrophila, J. Bacteriol. 164: 263.

    Google Scholar 

  • Doran, J.L, Bingle, W.H., and Page, W.J., 1987, Role of calcium in assembly of the Azotobacter vinelandii surface array, J. Gen. Microbiol. 133: 399.

    CAS  Google Scholar 

  • Edwards, P., and Smit, J., 1991, A transducing bacteriophage for Caulobacter crescentus uses the paracrystalline surface layer protein as a receptor, J. Bacteriol. 173: 5568.

    PubMed  CAS  Google Scholar 

  • Gilchrist, A., Fisher, J.A., and Smit, J., 1992, Nucleotide sequence analysis of the gene encoding the Caulobacter crescentus paracrystalline surface layer protein, Can. J. Microbiol. 38: 193.

    Article  PubMed  CAS  Google Scholar 

  • Hitchcock, P.J., and Brown,T.M., 1983, Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol. 154: 269.

    PubMed  CAS  Google Scholar 

  • Kasper, D.L, Weintraub, A., Lindberg, A.A., and Lonngren, J., 1983, Capsular polysaccharides and lipopolysaccharides from two Bacteroides fragilis strains: chemical and immunochemical characterization, J. Bacteriol. 153: 991.

    PubMed  CAS  Google Scholar 

  • Koval, S.F., and, Murray R.G.E., 1984, The isolation of surface array proteins from bacteria, Can. J. Biochem. Cell Biol. 62: 1181.

    Google Scholar 

  • Koval, S.F., and, Murray R.G.E., 1985, Effect of calcium on the in vivo assembly of the surface protein of Aquaspirillum serpens VHA, Can. J. Microbiol. 31: 261.

    CAS  Google Scholar 

  • Logan, S.M., and Trust, T.J., 1984, Structural and antigenic heterogeneity of lipopolysaccharides of Campylobacter jejuni and Campylobacter coli, Infect. Immun. 45: 210.

    Google Scholar 

  • Peterson, A. A., and McGroarty, E.M., 1985, High molecular weight components in lipopolysaccharides of Salmonella typhimurium, Salmonella minnesota, and Escherichia coli, J. Bacteriol. 162: 738.

    PubMed  CAS  Google Scholar 

  • Ravenscroft, N., Walker, S.G., Dutton, G.G.S., and Smit, J., 1992, Identification, isolation and structural studies of the outer membrane lipopolysaccharide of Caulobacter crescentus, J. Bacteriol.,in press.

    Google Scholar 

  • Sleytr, U. B., and Messner, P., 1988, Crystalline surface layers in procaryotes, J. Bacteriol. 170: 2891.

    Google Scholar 

  • Smit, J., Grano, D.A., Glaser, R.M., and Agabian, N., 1981, Periodic surface array in Caulobacter crescentus: fine structure and chemical analysis, J. Bacteriol. 146: 1135.

    PubMed  CAS  Google Scholar 

  • Smit, J., Engelhardt, H., Volker, S., Smith, S.H., and Baumeister, W., 1992, The Slayer of Caulobacter crescentus: Three-dimensional image reconstruction and structural analysis by electron microscopy, J. Bacteriol. 174: 6527.

    PubMed  CAS  Google Scholar 

  • Thorne, K.J.I., Thornley, M.J., Naisbitt, P., and Glauert, A.M., 1975, The nature of the attachment of a regularly arranged surface protein to the outer membrane of an Acinetobacter sp., Biochem. Biophys. Acta 389: 97.

    Article  PubMed  CAS  Google Scholar 

  • Walker, S.G., Ravenscroft, N., and Smit, J, 1991, Isolation of the cell surface molecules of Caulobacter crescentus, Proceed. Can. Soc. Microbiol., Western Branch Annu. Meet., Vol. 22 abstr. II - 41.

    Google Scholar 

  • Walker, S.G., Smith, S.H., and Smit, J., 1992, Isolation and comparison of the paracrystalline surface layer proteins of freshwater Caulobacters, J. Bacteriol. 174: 1783.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1993 Springer Science+Business Media New York

About this chapter

Cite this chapter

Walker, S.G., Smit, J. (1993). Attachment of the S-Layer of Caulobacter crescentus to the Cell Surface. In: Beveridge, T.J., Koval, S.F. (eds) Advances in Bacterial Paracrystalline Surface Layers. NATO ASI Series, vol 252. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9032-0_29

Download citation

  • DOI: https://doi.org/10.1007/978-1-4757-9032-0_29

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-9034-4

  • Online ISBN: 978-1-4757-9032-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics