Does the S-Layer of Aeromonas salmonicida Exist in More Than One Functional Organizational State?
The S-layer of the fish pathogen Aeromonas salmonicida, the causative agent of furunculosis in salmonids, is probably the best characterized S-layer in regards to function. Not only does it constitute an important determinant in pathogenesis (Ishiguro et al., 1981), but it has also been assigned specific virulence functions (reviewed by Kay and Trust, 1991). We have recently demonstrated that the S-layer of A. salmonicida (or A-layer) possesses significant flexibility and plasticity. It normally exists in a single type of lattice capable of adopting different conformations (Garduño and Kay, 1992a), but under calcium depletion it forms two novel structural patterns as a consequence of structural rearrangements within the layer (Garduño et al., 1992). It is the objective of this present work to explore the functional relevance of one of these novel structural patterns, the “big squares” pattern.
KeywordsStructural Pattern Fish Pathogen Functional Competence Calcium Depletion Significant Flexibility
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- Garduno, T.A., Phipps, B.M., Baumeister, W., and Kay W.W., 1992, Novel structural patterns in divalent cation-depleted surface layers of Aeromonas salmonicida, J. Struct. Biol. 109:(in press).Google Scholar