Abstract
Methanoculleus marisnigri is an irregularly-shaped coccus originally isolated by Romesser et al. (1979) from the anoxic sediment of the Black Sea. Cells are peritrichously flagellated, and grow optimally at 37° C. The cell wall is composed exclusively of a glycosylated S-layer with hexagonal symmetry. The S-layer protein contains (based on dry weight) 0.8% galactose, 0.3% glucose, and 0.1% mannose (Romesser et al., 1979) and has a Mr = 138 kDa (Zabel et al., 1984). We have isolated the S-layer glycoprotein of this organism by utilizing its sensitivity to Triton X-100 at elevated temperatures.
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Bayley, D.P., Koval, S.F. (1993). Effect of Triton X-100 on the S-Layer of Methanoculleus marisnigri. In: Beveridge, T.J., Koval, S.F. (eds) Advances in Bacterial Paracrystalline Surface Layers. NATO ASI Series, vol 252. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9032-0_26
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DOI: https://doi.org/10.1007/978-1-4757-9032-0_26
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