Abstract
The three-dimensional structures of many crystalline bacterial surface layers (S-layers) have been studied by electron microscopy. Protein molecules are composed of light elements (mainly C,O,N and H) which give very little contrast and are so sensitive to radiation damage in the electron microscope that it is impossible to observe many details in a protein molecule before it has been destroyed by the electron beam. There are two ways to overcome this dilemma. One is to embed the protein in a staining medium which both increases the contrast and resists radiation damage. The other way is to take micrographs using very low electron doses, before the protein molecules are completely destroyed. Images of hundreds of these “noisy” protein molecules can then be averaged into one image. These two methods can be used individually or in combination with one another.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Baumeister, W., Lembcke, G., Dürr, R. and Phipps, B., 1991, Electron crystallography of bacterial surface proteins, in: “Electron Crystallography of Organic Molecules”, J.R.Fryer and D.LDorset, eds., Kluwer Academic Publishers, Dordrecht.
Baumeister, W. and Engelhardt, H., 1987, Three-dimensional structure of bacterial surface layers, in: “Electron Microscopy of Proteins”, Vol. 6, R. Harris, ed., Academic Press, London.
Brändén, C.I. and Tooze, J., 1991, “Introduction to Protein Structure”, Garland, New York.
Deatherage, J.F., Taylor, K.A. and Amos, LA., 1983, Three-dimensional arrangement of the cell wall protein of Sulfolobus acidocaldarius, J. Mol. Biol. 167: 823.
Deisenhofer, J., Epp, O., Miki, K., Huber, R. and Michel, H., 1984, X-ray structure analysis of a membrane protein complex: Electron density map at 3A resolution and a model of the chromophores of the photosynthetic reaction center from Rhodopseudomonas viridis. J. Mol. Biol. 180: 385.
Finch, J.T., Klug, A. and Nermut, M.V., 1967, The structure of the macromolecular units on the cell walls of Bacillus polymxa, J. Cell Sci. 2: 587.
Henderson, R., Baldwin, J.M., Ceska, T.A., Zemlin, F., Beckman, E. and Downing, K.H., 1990, Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy, J. Mol. Biol. 213: 899.
Hovmöller, S., 1992, CRISP: crystallographic image processing on a personal computer, Ultramicroscopy 41: 121.
Hovmöller, S., Sjögren, A. and Wang, D.N., 1988, The structure of crystalline bacterial surface layers, Progr. Biophys. Molec. Biol. 51: 131.
Jap, B.K., Downing, K.H. and Walian, P.J., 1990, Structure of PhoE porin in projection at 3.5 A resolution, J. Struc. Biol. 103: 57.
Sjögren, A., Hovmöller, S., Farrants, G., Ranta, H., Haapasalo, M., Ranta, K., and Lounatmaa, K., 1985, Structures of two different surface layers found in six Bacteroides strains, J. Bacteriol. 164: 1278.
Weiss, M.S., Wacker, T., Weckesser, J., Welte, W. and Schulz, G.E., 1990, The three-dimensional structure of porin from Rhodobacter capsulatus at 3 A resolution, FEBS Lett. 267: 268.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Springer Science+Business Media New York
About this chapter
Cite this chapter
Hovmöller, S. (1993). Crystallographic Image Processing Applications for S-Layers. In: Beveridge, T.J., Koval, S.F. (eds) Advances in Bacterial Paracrystalline Surface Layers. NATO ASI Series, vol 252. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9032-0_2
Download citation
DOI: https://doi.org/10.1007/978-1-4757-9032-0_2
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-9034-4
Online ISBN: 978-1-4757-9032-0
eBook Packages: Springer Book Archive