A Perspective on S-Layer Research

  • R. G. E. Murray
Chapter
Part of the NATO ASI Series book series (NSSA, volume 252)

Abstract

It is almost forty years since Houwink (1953) demonstrated by electron microscopy a monolayer of macromolecules in a regular array, which we would now call an S-layer, on the external surface of the cell wall of an unidentified Spirillum. It was an elegant oddity for only a short time; within three years shadow-cast preparations displayed arrays on representatives of four more distinct genera. An ever increasing number of genera and species have been added to the list and continue to be recognized as having hexagonal (p6), tetragonal (p4), trimeric (p3), and oblique (p2) lattice forms; to these must be added some with layer upon layer, even with different symmetries expressed in each layer. Messner and Sleytr (1992) have tabulated from the literature more than 300 strains representing some 93 genera belonging to all the major phylogenetic groups of prokaryotes including Archaea. Forty years of research have generated a substantial body of knowledge which is available in several recent reviews on S-layers (Messner and Sleytr, 1992; Koval, 1988; Smit, 1986) as well as on the range of surface components of bacterial walls (Beveridge and Graham, 1991). More specialized areas of study include the self-assembly of units to form S-layers (Sleytr and Messner, 1989), participation of carbohydrates in the macromolecules (Messner and Sleytr, 1991), and the analysis of computer-enhanced micrographic images with generation of 3-D models (Hovmöller et al., 1988). The status of work in hand at the time of the previous workshop is published in a book (Sleytr et al., 1988) and this present volume is for current observations and concepts.

Keywords

Bacterial Cell Wall Surface Component Deinococcus Radiodurans Bacterial Wall Major Phylogenetic Group 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Austin, J.W., and Murray, R.G.E., 1990, Isolation and in vitro assembly of the components of the outer S-layer of Lampropedia hyalina, J. Bacteriol. 173:3681.Google Scholar
  2. Beveridge, T.J., and Graham, L, 1991, Surface layers of bacteria, Microbiol. Rev. 55: 684.PubMedGoogle Scholar
  3. Boivin, M.F., Morris, V.L, Lee-Chan, E.C.M., and Murray, R.G.E., 1985, Deoxyribonucleic acid relatedness between selected members of the genus Aquaspirillum by slot/blot hybridization: Aquaspirillum serpens (Mueller 1786) Hylemon, Wells, Krieg, and Jannasch 1973 emended to included Aquaspirillum bengal as a subjective synonym, Int. J. System. Bacteriol. 35: 512.Google Scholar
  4. Brenner, S., and Horne, R.W., 1959, A negative staining method for high resolution electron microscopy of viruses, Biochim. Biophys. Acta 34: 103.PubMedCrossRefGoogle Scholar
  5. Chapman, J.A., Murray, R.G.E., and Salton, M.R.J., 1963, The surface anatomy of Lampropedia hyalina, Proc. Roy. Soc. London Ser. B., 158: 498.CrossRefGoogle Scholar
  6. Chart, H., Shaw, D.H., Ishiguro, E.E., and Trust, T.J., 1984, Structural and immunochemical homogeneity of Aeromonas salmonicida lipopolysaccharide, J. Bacteriol. 158: 16.Google Scholar
  7. Elmes, M.L, Scraba, D.G., and Weiner, J.H., 1986, Isolation and characterization of the tubular organelles induced by fumarate reductase overproduction in Escherichia coli, J. Gen. Microbiol. 132: 1429.PubMedGoogle Scholar
  8. Fitz-James, P.C., 1953, The structure of spores as revealed by mechanical disruption, J. Bacteriol. 66: 312.PubMedGoogle Scholar
  9. Houwink, A.L, 1953, A macromolecular mono-layer in the cell wall of Spirillum spec., Biochim. Biophys. Acta 10: 360.PubMedCrossRefGoogle Scholar
  10. Hovmöller, S., Sjögren, A., and Wang, D.N., 1988, The structure of crystalline bacterial surface layers, Prog. Biophys. Molec. Biol. 51: 131.CrossRefGoogle Scholar
  11. Kellenberger, E., and Ryter, A., 1958, Cell wall and cytoplasmic membranes of Escherichia coli, J. Biophys. Biochem. Cytol. 4: 323.PubMedCrossRefGoogle Scholar
  12. Kist, M.L, and Murray, R.G.E., 1981, Cell wall proteins of Aquaspirillum serpens, J. Bacteriol. 146: 1083.Google Scholar
  13. Koval, S.F., and Murray, R.G.E., 1986, The superficial protein arrays on bacteria, Microbiol. Sci. 3: 357.PubMedGoogle Scholar
  14. Koval, S.F., 1988, Paracrystalline protein surface arrays on bacteria, Can. J. Microbiol. 34: 407.CrossRefGoogle Scholar
  15. Listgarten, M.A., and Lai, C H, 1975, Unusual cell wall ultrastructure of Leptotrichia buccalis, J. Bacteriol. 123: 747.PubMedGoogle Scholar
  16. Messner, P., Hollaus, F., and Sleytr, U.B., 1984, Paracrystalline cell wall surface layers of different Bacillus stearothermophilus strains, Int. J. Syst. Bacteriol. 34: 202.CrossRefGoogle Scholar
  17. Messner, P., and Sleytr, U.B., 1991, Bacterial surface layer glycoproteins, Glycobiol. 1: 545.CrossRefGoogle Scholar
  18. Messner, P., and Sleytr, U.B., 1992, Crystalline bacterial cell-surface layers, Adv. Microbial. Physiol. 33: 213.CrossRefGoogle Scholar
  19. Murray, R.G.E., 1963, Role of superficial structures in the characteristic morphology of Lampropedia hyalina, Can. J. Microbiol. 9: 593.CrossRefGoogle Scholar
  20. Salton, M.R.J., and Horne, R.W., 1951, Studies on the bacterial cell wall. II. Methods of preparation and some properties of cell wall, Biochim. Biophys. Acta 7: 177.PubMedCrossRefGoogle Scholar
  21. Salton, M.R.J., 1953, Studies of the bacterial cell wall. IV. The composition of the cell walls of some gram-positive and gram-negative bacteria, Biochim. Biophys. Acta 10: 512.PubMedCrossRefGoogle Scholar
  22. Sleytr, U.B., and Plohberger, R., 1980, The dynamic process of assembly of two dimensional arrays of macromolecules on bacterial cell walls, in: “Electron Microscopy at Molecular Dimensions”, W. Baumeister and W. Vogell, ed., pp. 36–47, Springer-Verlag, Berlin.CrossRefGoogle Scholar
  23. Sleytr, U.B., Messner, P., Pum, D., and Sara, M. (ed.), 1988, “Crystalline Bacterial Cell Surface Layers”, Springer-Verlag, Berlin.Google Scholar
  24. Sleytr, U.B., and Messner, P., 1989, Self-assembly of crystalline bacterial cell surface layers (S-layers), in: “Electron Microscopy of Subcellular Dynamics”, H. Plattner, ed., pp. 13–31. CRC Press, Boca Raton.Google Scholar
  25. Smit, J., 1986, Protein surface layers, in: “Bacterial Outer Membranes as Model Systems”, M. Inouye, ed., pp. 343–376, John Wiley and Sons, Chichester. Smith, S.H., and Murray, R.G.E., 1990, The structure and associations of the doubleGoogle Scholar
  26. S-layer on the cell wall of Aquaspirillum sinuosum, Can. J. Microbiol. 36:327.Google Scholar
  27. Stanier, R.Y., and van Niel, C.B., 1962, The concept of a bacterium. Arch. Microbiol. 42: 17.Google Scholar
  28. Thompson, B.G., Murray, R.G.E., and Boyce, J.F., 1982, The association of the surface array and the outer membrane of Deinococcus radiodurans, Can. J. Microbiol. 28: 1081.CrossRefGoogle Scholar
  29. Walsby, A.E., 1972, Structure and function of gas vacuoles, Bacteriol. Rev. 36: 1.Google Scholar
  30. Weidel, W., Frank, H., and Martin, H.H., 1960, The rigid layer of the cell wall of Escherichia coli strain B, J. Gen. Microbiol. 22: 158.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • R. G. E. Murray
    • 1
  1. 1.Department of Microbiology and ImmunologyUniversity of Western OntarioLondonCanada

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