Bovine in Vitro Fertilization

  • J. J. Parrish
  • N. L. First


In vitro fertilization in cattle was first reported by Bracket et al. (1982) using surgically recovered oocytes that were matured in vivo and surgically transferred as zygotes to the oviducts of cows. The procedure was later simplified by laparoscopic removal of in vivo-matured oocytes and development of the embryos in oviducts of rabbits (Sirard and Lambert, 1985; Sirard et al., 1985). Although successful, these experiments were not highly efficient in sperm capacitation and required in vivo oocyte maturation, surgery, and development of the embryos in oviducts. Recently systems for producing and culturing bovine embryos totally in vitro have been developed. As a result, bovine embryos are now produced in large numbers for research purposes or in some cases for commercial embryo transfer (Leibfried-Rutledge et al., 1989; Gordon and Lu, 1990). The efficient production of embryos in vitro has required an understanding of the mechanisms of sperm capacitation, maturation of oocytes, and sperm-egg interaction in the fertilization process. Understanding these processes for bovine gametes is the focus of this review.


Follicular Fluid Seminal Plasma Oocyte Maturation Zona Pellucida Cumulus Cell 
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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • J. J. Parrish
    • 1
  • N. L. First
    • 1
  1. 1.Department of Meat and Animal ScienceUniversity of WisconsinMadisonUSA

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