Fertilization in the Guinea Pig

  • George L. Gerton


Since the study of guinea pig fertilization and early development began over 137 years ago (see references cited by Hunter et al., 1969), the guinea pig has been an important experimental animal for investigators trying to understand mechanisms of sperm—egg interaction that lead to fertilization. The purpose of this review is not to provide an exhaustive treatise on the subject. Rather, advantages and disadvantages of the guinea pig system are discussed with particular reference to specific areas of research that have received considerable attention. In addition, prospective areas for future investigations are highlighted.


Zona Pellucida Acrosome Reaction Apical Segment Testicular Sperm Sperm Capacitation 
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  1. Anakwe, O. O., Sharma, S., Hoff, H. B., Hardy, D. M., and Gerton, G. L., 1991, Maturation of guinea pig sperm in the epididymis involves the modification of proacrosin oligosaccharide side-chains, Molec. Reprod. Dev. 29: 294–301.PubMedCrossRefGoogle Scholar
  2. Aonuma, S., Mayumi, T., Suzuki, K., Noguchi, T., Iwai, M., and Okabe, M., 1973, Studies on sperm capacitation. I. The relationship between guinea pig sperm coating antigen and a sperm capacitation phenomenon, J. Reprod. Fertil. 35: 425–432.PubMedCrossRefGoogle Scholar
  3. Arboleda, C. E., and Gerton, G. L., 1988, Proacrosin/acrosin during guinea pig spermatogenesis, Dev. Biol. 125: 217–225.PubMedCrossRefGoogle Scholar
  4. Austin, C. R., 1961, Fertilization of eggs in vitro, Int. Rev. Cytol. 12: 337–359.PubMedCrossRefGoogle Scholar
  5. Bhattacharyya, A., and Yanagimachi, R., 1988, Synthetic organic pH buffers can support fertilization of guinea pig eggs, but not as efficiently as bicarbonate buffer, Gamete Res. 19: 123–129.PubMedCrossRefGoogle Scholar
  6. Blaquier, J. A., Cameo, M. S., and Burgos, M. H., 1972, The role of androgens in the maturation of epididymal spermatozoa in the guinea pig, Endocrinology 90: 839–842.PubMedCrossRefGoogle Scholar
  7. Bleil, J. D., and Wassarman, P M., 1986, Autoradiographic visualization of the mouse egg’s sperm receptor bound to sperm, J. Cell Biol. 102: 1363–1371.PubMedCrossRefGoogle Scholar
  8. Coronel, C. E., San Augustin, J., and Lardy, H. A., 1988, Identification and partial characterization of caltrin-like proteins in the reproductive tract of the guinea pig, Biol. Reprod. 38: 713–722.PubMedCrossRefGoogle Scholar
  9. Dyson, A. L. M. B., and Orgebin-Crist, M.-C., 1973, Effect of hypophysectomy, castration and androgen replacement upon the fertilizing ability of rat epididymal spermatozoa, Endocrinology 93: 391–402.PubMedCrossRefGoogle Scholar
  10. Fawcett, D. W, and Hollenberg, R. D., 1963, Changes in the acrosome of guinea pig spermatozoa during passage through the epididymis, Z. Zellforsch. 60: 276–292.PubMedCrossRefGoogle Scholar
  11. Flaherty, S. P, and Olson, G. E., 1988, Membrane domains in guinea pig sperm and their role in the membrane fusion events of the acrosome reaction, Anat. Rec. 220: 267–280.PubMedCrossRefGoogle Scholar
  12. Fleming, A. D., and Armstrong, D. T., 1985, Effects of polyamines upon capacitation and fertilization in the guinea pig, J. Exp. Zool. 233: 93–100.PubMedCrossRefGoogle Scholar
  13. Fleming, A. D., and Kuehl, T. J., 1985, Effects of temperature upon capacitation of guinea pig spermatozoa, J. Exp. Zool. 233: 405–411.PubMedCrossRefGoogle Scholar
  14. Fleming, A. D., and Yanagimachi, R., 1984, Evidence suggesting the importance of fatty acids and the fatty acid moieties of sperm membrane phospholipids in the acrosome reaction of guinea pig spermatozoa, J. Exp. Zool. 229: 485–489.PubMedCrossRefGoogle Scholar
  15. Friend, D. S., 1982, Plasma-membrane diversity in a highly polarized cell, J. Cell Biol. 93: 243–249.PubMedCrossRefGoogle Scholar
  16. Friend, D. S., Orci, L., Perrelet, A., and Yanagimachi, R., 1977, Membrane particle changes attending the acrosome reaction in guinea pig spermatozoa, J. Cell Biol. 74: 561–577.PubMedCrossRefGoogle Scholar
  17. Green, D. P. L., 1978, The induction of the acrosome reaction in guinea-pig sperm by the divalent metal cation ionophore A23187, J. Cell Sci. 32: 137–151.PubMedGoogle Scholar
  18. Horan, A. H., and Bedford, J. M., 1972, Development of the fertilizing ability of spermatozoa in the epididymis of the Syrian hamster, J. Reprod. Fertil. 30: 417–423.PubMedCrossRefGoogle Scholar
  19. Huang, T. T. F., and Yanagimachi, Y., 1984, Fucoidin inhibits attachment of guinea pig spermatozoa to the zona pellucida through binding to the inner acrosomal membrane and equatorial domains, Exp. Cell Res. 153: 363–373.PubMedCrossRefGoogle Scholar
  20. Huang, T. T. E, Fleming, A. D., and Yanagimachi, Y., 1981, Only acrosome-reacted spermatozoa can bind and penetrate into zona pellucida: A study using guinea pig, J. Exp. Zool. 217: 286–290.CrossRefGoogle Scholar
  21. Huang, T. T. E, Ohzu, E., and Yanagimachi, Y., 1982, Evidence suggesting that L-fucose is part of a recognition signal for sperm—zona pellucida attachment in mammals, Gamete Res. 5: 355–361.CrossRefGoogle Scholar
  22. Huang, T. T. F., Hardy, D., Yanagimachi, H., Teuscher, C., Ring, K., Wild, G., and Yanagimachi, R., 1985, pH and protease control of acrosomal content stasis and release during the guinea pig sperm acrosome reaction, Biol. Reprod. 32: 451–462.Google Scholar
  23. Hunter, R. H. E, Hunt, D. M., and Chang, M. C., 1969, Temporal and cytological aspects of fertilization and early development in the guinea pig, Cavia porcellus, Anat. Rec. 165: 411–430.PubMedCrossRefGoogle Scholar
  24. Hyne, R. V, 1984, Bicarbonate and calcium-dependent induction of rapid guinea pig sperm acrosome reactions by monovalent ionophores, Biol. Reprod. 31: 312–323.PubMedCrossRefGoogle Scholar
  25. Hyne, R. V., and Garbers, D. L., 1979, Calcium-dependent increase in adenosine 3’,5’-monophosphate and induction of the acrosome reaction in guinea pig spermatozoa, Proc. Natl. Acad. Sci. USA 76: 5699–5703.PubMedCrossRefGoogle Scholar
  26. Hyne, R. V., and Garbers, D. L., 1981, Requirement of serum factors for capacitation and the acrosome reaction of guinea pig spermatozoa in buffered medium below pH 7.8, Biol. Reprod. 24: 257–266.PubMedCrossRefGoogle Scholar
  27. Jagiello, G. M., 1969, Some cytologic aspects of meiosis in female guinea pig, Chromosoma 27: 95–101.PubMedCrossRefGoogle Scholar
  28. Joyce, C. L., Nuzzo, N. A., Wilson, L., Jr., and Zaneveld, L. J. D., 1987, Evidence for a role of cyclooxygenase (prostaglandin synthetase) and prostaglandins in the sperm acrosome reaction, J. Androl. 8: 74–82.PubMedGoogle Scholar
  29. Lawson, D., Fewtrell, C., and Raff, M., 1978, Localized mast cell degranulation induced by concanavalin Asepharose beads: Implications for the Cat+ hypothesis of stimulus—secretion coupling, J. Cell Biol. 79: 394–400.PubMedCrossRefGoogle Scholar
  30. Loeb, L., 1911, The cyclic changes in the ovary of the guinea pig, J. Morphol. 22: 39–70.CrossRefGoogle Scholar
  31. Loeb, L., 1914, The correlation between the cyclic changes in the uterus and ovaries in the guinea pig, Biol. Bull. 27: 1–44.CrossRefGoogle Scholar
  32. Loeb, L., 1918, Corpus luteum and the periodicity in the sexual cycle, Science 48: 273–277.PubMedCrossRefGoogle Scholar
  33. Myles, D. G., and Primakoff, P, 1984, Localized surface antigens of guinea pig sperm migrate to new regions prior to fertilization, J. Cell Biol. 99: 1634–1641.PubMedCrossRefGoogle Scholar
  34. Myles, D. G., Primakoff, P., and Bellvé, A. R., 1981, Surface domains of the guinea pig sperm defined with monoclonal antibodies, Cell 23: 433–439.PubMedCrossRefGoogle Scholar
  35. Myles, D. G., Primakoff, P, and Koppel, D. E., 1984, A localized surface protein of guinea pig sperm exhibits free diffusion in its domain, J. Cell Biol. 98: 1905–1908.PubMedCrossRefGoogle Scholar
  36. Myles, D. G., Hyatt, H., and Primakoff, P, 1987, Binding of both acrosome-intact and acrosome-reacted guinea pig sperm to the zona pellucida during in vitro fertilization, Dev. Biol. 121: 559–567.PubMedCrossRefGoogle Scholar
  37. Nagae, T., and Srivastava, P. N., 1986, Induction of the acrosome reaction in guinea pig spermatozoa by calmodulin antagonist W-7, Gamete Res. 14: 197–208.CrossRefGoogle Scholar
  38. Noland, T. D., Davis, L. S., and Olson, G. E., 1989, Regulation of proacrosin conversion in isolated guinea pig sperm acrosomal apical segments, J. Biol. Chem. 264: 13586–13590.PubMedGoogle Scholar
  39. Olson, G. E., Winfrey, V. P., Winer, M. A., and Davenport, G. R., 1987, Outer acrosomal membrane of guinea pig spermatozoa: Isolation and structural characterization, Gamete Res. 17: 77–94.PubMedCrossRefGoogle Scholar
  40. Perreault, S. D., Zirkin, B. R., and Rogers, B. J., 1982, Effect of trypsin inhibitors on acrosome of guinea pig spermatozoa, Biol. Reprod. 26: 343–351.PubMedCrossRefGoogle Scholar
  41. Phelps, B. M., and Myles, D. G., 1987, The guinea pig sperm plasma membrane protein, PH-20, reaches the surface via two transport pathways and becomes localized to a domain after an initial uniform distribution, Dev. Biol. 123: 63–72.PubMedCrossRefGoogle Scholar
  42. Phelps, B. M., Primakoff, P. M., Koppel, D. E., Low, M. G., and Myles, D. G., 1988, Restricted lateral diffusion of PH-20, a PI-anchored sperm membrane protein, Science 240: 1780–1782.PubMedCrossRefGoogle Scholar
  43. Primakoff, P., and Myles, D. G., 1983, A map of the guinea pig sperm surface constructed with monoclonal antibodies, Dev. Biol. 98: 417–428.PubMedCrossRefGoogle Scholar
  44. Primakoff, P., Myles, D. G., and Bellvé, A. R., 1980, Biochemical analysis of the released products of the mammalian acrosome reaction, Dev. Biol. 80: 324–331.PubMedCrossRefGoogle Scholar
  45. Primakoff, P., Hyatt, H., and Myles, D. G., 1985, A role for the migrating sperm surface antigen PH-20 in guinea pig sperm binding to the egg zona pellucida, J. Cell Biol. 101: 2239–2244.PubMedCrossRefGoogle Scholar
  46. Primakoff, P, Hyatt, H., and Tredick-Kline, J., 1987, Identification and purification of a sperm surface protein with a potential role in sperm–egg membrane fusion, J. Cell Biol. 104: 141–149.PubMedCrossRefGoogle Scholar
  47. Primakoff, E, Lathrop, W., Woolman, L., Cowan, A., and Myles, D., 1988, Fully effective contraception in male and female guinea pigs immunized with the sperm protein PH-20, Nature 335: 543–546.PubMedCrossRefGoogle Scholar
  48. Schenk, S. L., 1878, Das Sangethierei kunstlich befruchtet ausserhalb des Mutter thieves, Mitt. Embryol. Inst. K.K. Univ. Wien 1: 107–118.Google Scholar
  49. Schwarz, M. A., and Koehler, J. K., 1979, Alterations in lectin binding to guinea pig spermatozoa accompanying in vitro capacitation and the acrosome reaction, Biol. Reprod. 21: 1295–1307.PubMedCrossRefGoogle Scholar
  50. Sharon, N., 1975, Complex Carbohydrates, Addison-Wesley, Reading, MA, pp. 155–166.Google Scholar
  51. Shi, Q. X., and Friend, D. S., 1985, Effect of gossypol acetate on guinea pig epididymal spermatozoa in vivo and their susceptibility to capacitation in vitro, J. Androl. 6: 45–62.PubMedGoogle Scholar
  52. Singh, J. P., Babcock, D. E, and Lardy, H. F., 1978, Increased calcium ion influx is a component of capacitation of spermatozoa, Biochem. J. 172: 549–556.PubMedGoogle Scholar
  53. Srivastava, P. N., Kumar, V. M., and Arbtan, K. D., 1988, Neuraminidase induces capacitation and acrosome reaction in mammalian spermatozoa, J. Exp. Zool. 245: 106–110.PubMedCrossRefGoogle Scholar
  54. Stigler, R., 1918, Der Einfluss des Nebenhodens auf die Vitalität der Spermatozoen, Pflügler’s Arch. 171: 273–282.CrossRefGoogle Scholar
  55. Stockard, C. R., and Papacinolaou, G., 1917, The existence of a typical oestrous cycle in the guinea pig—with a study of its histological and physiological changes, Am. J. Anat. 22: 225–265.CrossRefGoogle Scholar
  56. Stockard, C. R., and Papacinolaou, G., 1919, The vaginal closure membrane, copulation, and the vaginal plug in the guinea-pig, with further considerations of the oestrous rhythym, Biol. Bull. 37: 222–245.CrossRefGoogle Scholar
  57. Stojanoff, A., Bourne, H., Andrews, A. G., and Hyne, R. V, 1987, Isolation of a stable apical segment of the guinea pig sperm acrosome, Gamete Res. 17: 321–332.PubMedCrossRefGoogle Scholar
  58. Summers, R. G., Talbot, P., Keough, E. M., Hylander, B. L., and Franklin, L. E., 1976, Ionophore A23187 induces acrosome reactions in sea urchin and guinea pig spermatozoa, J. Exp. Zool. 196: 381–385.PubMedCrossRefGoogle Scholar
  59. Talbot, P, and Franklin, L. E., 1978, Surface modifications of guinea pig sperm during in vitro capacitation: An assessment using lectin-induced agglutination of living sperm, J. Exp. Zool. 203: 1–14.PubMedCrossRefGoogle Scholar
  60. Wilson, W L., and Oliphant, G., 1987, Isolation and biochemical characterization of the subunits of the rabbit sperm acrosome stabilizing factor, Biol. Reprod. 37: 159–169.PubMedCrossRefGoogle Scholar
  61. Yanagimachi, R., 1972, Fertilization of guinea pig eggs in vitro, Anat. Rec. 174: 9–20.PubMedCrossRefGoogle Scholar
  62. Yanagimachi, R., 1974, Maturation and fertilization in vitro of guinea pig ovarian oocytes,. 1. Reprod. Fertil. 38: 485–488.CrossRefGoogle Scholar
  63. Yanagimachi, R., and Usui, N., 1974, Calcium dependence of the acrosome reaction and activation of guinea pig spermatozoa, Exp. Cell Res. 89: 161–174.PubMedCrossRefGoogle Scholar
  64. Young, W. C., 1927, The influence of high temperature on the guinea-pig testis: Histological changes and effects on reproduction, J. Exp. Zool. 49: 459–499.CrossRefGoogle Scholar
  65. Young, W. C., 1929a, A study of the function of the epididymis. I. Is the attainment of full spermatozoon maturity attributable to some specific action of the epididymal secretion? J. Morphol. Physiol. 47: 479–495.CrossRefGoogle Scholar
  66. Young, W. C., 1929b, A study of the function of the epididymis. II. The importance of an aging process in sperm for the length of the period during which fertilizing capacity is retained by sperm isolated in the epididymis of the guinea-pig, J. Morphol. Physiol. 48: 475–491.CrossRefGoogle Scholar
  67. Young, W. C., 1931, A study of the function of the epididymis. III. Functional changes undergone by spermatozoa during their passage through the epididymis and vas deferens in the guinea-pig, J. Expo. Biol. 8: 151–162.Google Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • George L. Gerton
    • 1
  1. 1.Division of Reproductive Biology, Department of Obstetrics and GynecologyUniversity of Pennsylvania School of MedicinePhiladelphiaUSA

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