Immobilization of Multienzymes of Urea Cycle into Fibrin Membrane: An Approach to an Artificial Liver

  • H. Hagiwara
  • T. Nagasaki
  • Y. Saito
  • Y. Inada
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 153)


The final obligatory step for the metabolism of nitrogeneous compounds in human body is the conversion of ammonia to urea through carbamyl phosphate and urea cycle with the aid of ATP. Accumulation of ammonia in human blood is observed for patients with liver failure and hepatic coma and gives rise to a serious damage to brain. The metabolic pathway for the urea synthesis from ammonia is shown below, which is mainly conducted in liver.


Urea Cycle Hepatic Coma Urea Synthesis Immobilize System Inorganic Pyrophosphatase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    O. Rochovansky and S. Ratner, J. Biol. Chem., 242: 3839 (1967)PubMedGoogle Scholar
  2. 2.
    L. Raijman and M. E. Jones, Arch. Biochem. Biophys., 175: 270 (1976)PubMedCrossRefGoogle Scholar
  3. 3.
    E. A. Havir, H. Tamir, S. Ratner and R. C. Warner, J. Biol. Chem., 240: 3079 (1965)PubMedGoogle Scholar
  4. 4.
    M. Nakamura and M. E. Jones, Methods in Enzymology, Vol. XVII-A, Academic Press, New York, (1970) p. 286.Google Scholar
  5. 5.
    R. T. Schimke, Methods in Enzymology, Vol. XVII-A, Academic Press, New York, (1970) p. 313Google Scholar
  6. 6.
    Y. Inada, S. Hirose, A. Matsushima, H. Mihama and Y. Hiramoto, Experientia, 33: 1257 (1977)PubMedCrossRefGoogle Scholar
  7. 7.
    Y. Inada, S. Hirose, M. Okada, and H. Mihama, Enzyme, 20: 188 (1975)PubMedGoogle Scholar
  8. 8.
    Y. Inada, S. Yamazaki, S. Miyake, S. Hirose, M. Okada and H. Mihama, Biochem. Biophys. Res. Commun., 67: 1275 (1975)PubMedCrossRefGoogle Scholar
  9. 9.
    H. Okamoto, S. Kanai, P. Tipayang and Y. Inada, Enzyme, 24: 273 (1979)PubMedGoogle Scholar
  10. 10.
    H. Okamoto, P. Tipayang and Y. Inada, Biochim. Biophys. Acta, 611: 35 (1980)PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1982

Authors and Affiliations

  • H. Hagiwara
    • 1
  • T. Nagasaki
    • 1
  • Y. Saito
    • 1
  • Y. Inada
    • 1
  1. 1.Laboratory of Biological ChemistryTokyo Institute of TechnologyOokayama, Meguroku, Tokyo 152Japan

Personalised recommendations