Acute Glucagon Treatment in Rats Fed Various Protein Diets Effect on N-Acetyl Glutamate Concentration

  • L. Cathelineau
  • D. Rabier
  • F. X. Coudé
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 153)


Carbamoyl phosphate synthetase (ammonia) (CPS) which is localized in mitochondria has an absolute requirement for N-acetyl glutamate (NAGA) as activator. Since the important work of Shigesada and Tatibanal the role of its intramitochondrial concentration upon the regulation of ureogenesis has been stressed by numerous workers: the levels of NAGA show a positive correlation with the ability of the liver or of the isolated hepatocytes to synthetize citrulline1,2,3,4. NAGA is closely localized into mitochondria, because it is destroyed in the cytosol by a powerful deacylase activity5 . Then, NAGA content in mitochondria must be appreciated either by measurements in the whole liver or by assay on isolated mitochondria. By the use of one of these two methods, it has been reported that low NAGA concentrations are obtained with short6 or prolonged3 hypo or aprotidic diets, with acute changes from 70 % to 5 % proteins in the diet2, with acute accumulation of toxic metabolites in the liver, propionate78 pent-4-enoate9 and valproate10 . Low NAGA concentrations are also observed during the last days of the gestation in rat females11. In contrary, high NAGA levels are obtained with short6 and prolonged3 hyperprotidic diets, with acute changes from 5 % to 70 % or protein in the food2, with the administration in vivo of arginine6 and also observed during the delivery of rat females11. Prolonged hyperprotidic diets increase the rate of N-acetyl glutamate synthetase activity12 while short term changes of protein amount in nutriments have no effect6 on it.


Carbohydrate Diet Carbamoyl Phosphate Synthetase Carbamyl Phosphate Naga Level Acetyl Glutamate 
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Copyright information

© Springer Science+Business Media New York 1982

Authors and Affiliations

  • L. Cathelineau
    • 1
  • D. Rabier
    • 1
  • F. X. Coudé
    • 1
  1. 1.Laboratoire de Biochimie GénétiqueHôpital Necker-Enfants MaladesParis Cedex 15France

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