Studies on the Mechanism of Estradiol Uptake by Rat Uterine Cells and on Estradiol Binding to Uterine Plasma Membranes

  • R. E. Muller
  • T. C. Johnston
  • A. M. Traish
  • H. H. Wotiz
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 117)


A method for the preparation of viable uterine cell suspensions is described. Using this system the kinetics of estradiol uptake were studied in order to assess whether the steroid enters uterine cells by passive diffusion (1) or protein mediated diffusion (2). The data presented show that a) the rates of estradiol entry are nonsaturable; b) temperature dependence of uptake kinetics gives a linear Arrhenius plot; c) E2-6-CMO-BSA does not inhibit 3H-E2 uptake; d) uterine plasma membranes do not contain specific estrogen binding sites. Thus, estrogen uptake occurs by passive diffusion.


Dextran Coated Charcoal Intact Uterus Estrogen Binding Cytosol Receptor Uterine Cell 
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Copyright information

© Springer Science+Business Media New York 1979

Authors and Affiliations

  • R. E. Muller
    • 1
  • T. C. Johnston
    • 1
  • A. M. Traish
    • 1
  • H. H. Wotiz
    • 1
  1. 1.Biochemistry DepartmentBoston University School of MedicineBostonUSA

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