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Novel Magnetic Microcarriers on the Basis of Poly(Vinyl Alcohol) for Biomedical Analysis

  • Chapter
Scientific and Clinical Applications of Magnetic Carriers

Abstract

Novel magnetic microspheres based on poly(vinyl alcohol) (PVA) were synthesized using a water-in-oil-suspension technique. The preparation method enables bead sizes from 1 to 1000 µm and represents a simple procedure requiring only a fraction of the time and effort usually necessary for the synthesis of magnetic beads. The unique hydrogel-like structure of PVA offers a high chemical functionality comparable to that of the well known agarose gels. The applicability of the new beads was demonstrated by two tests: (i) an immunoadsorption test to remove blood group antibodies from human plasma using blood group antigen coated beads and (ii) the binding of biotinylated albumin to differently coated avidin beads. The tests revealed a good binding performance for both carriers. The relationship between the binding capacity of the biotin probe and the avidin coupling mode is explained in more detail.

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Author information

Authors and Affiliations

  1. RWTH Aachen, Institut für Anorganische Chemie, Professor-Pirlet-Str.1, D-52074, Aachen, Germany

    Detlef Müller-Schulte & Frank Füssl

  2. Campus Kortrijk, Katholieke Universiteit Leuven, B-8500, Kortrijk, Belgium

    Marcel De Cuyper

Authors
  1. Detlef Müller-Schulte
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  2. Frank Füssl
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  3. Marcel De Cuyper
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Editor information

Editors and Affiliations

  1. The Cleveland Clinic Foundation, Cleveland, Ohio, USA

    Urs Häfeli  & Maciej Zborowski  & 

  2. University of Rostock, Rostock, Germany

    Wolfgang Schütt

  3. micro caps, Rostock, Germany

    Joachim Teller

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© 1997 Springer Science+Business Media New York

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Müller-Schulte, D., Füssl, F., De Cuyper, M. (1997). Novel Magnetic Microcarriers on the Basis of Poly(Vinyl Alcohol) for Biomedical Analysis. In: Häfeli, U., Schütt, W., Teller, J., Zborowski, M. (eds) Scientific and Clinical Applications of Magnetic Carriers. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-6482-6_7

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  • DOI: https://doi.org/10.1007/978-1-4757-6482-6_7

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4419-3283-9

  • Online ISBN: 978-1-4757-6482-6

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