Superparamagnetic Iron Oxide Particles as Marker Substances for Searching Tumor Specific Liposomes with Magnetic Resonance Imaging

  • Sabine Päuser
  • Regina Reszka
  • Susanne Wagner
  • Karl-Jürgen Wolf
  • Heinz Johannes Buhr
  • Gerd Berger


Superparamagnetic iron oxide particles (SPIOs) encapsulated with oleic acid were incorporated within the lipid phase of different types of liposomes and served as markers in a search for tumor specific drug-carriers by noninvasive Magnetic Resonance Tomography (MRT). The experiments were performed on the model CC531 adenocarcinoma within the liver of WAG/RIJ rats. The reduction of the relative signal intensity (SI) in the tumor in T’2-weighted MR images was assumed as a measure of the liposome enrichment in the tumor or adjacent tissue. The liposome-encapsulated SPIOs were investigated and compared to AMI-227, dextran-coated SPIOs in two different doses, at isomolar doses regarding their iron content and at isodoses concerning their MR relaxivity R2. Reverse phase evaporation vesicles and small unilamellar vesicles showed a remarkably different behavior depending on the applied dose. A steadily strong SI-reduction, starting immediately after injection and extending up to 48 hours was observed for small unilamellar vesicles sterically stabilized with polyethylene glycol.


Superparamagnetic Iron Oxide Positron Emission Tomography4 Liposome Preparation Relative Signal Intensity Diisopropyl Ether 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Huang L (Ed.) (1994). Forum Liposomes in diagnostic imaging. In Journal of Liposome Research 4, 741–1015.CrossRefGoogle Scholar
  2. 2.
    Kim S (1993). Liposomes as carriers of cancer chemotherapy. Drugs 46,618–638.CrossRefGoogle Scholar
  3. 3.
    Gregoriades G (Ed.) (1992). Liposome Technology. CRC Press: Florida.Google Scholar
  4. 4.
    Oku N, Tokudome Y, Tsukada H et al (1995). Real-time analysis of liposomal trafficking in tumor-bearing mice by use of positron emission tomography. Biochimia et Biophysica Acta 1238, 86–90.CrossRefGoogle Scholar
  5. 5.
    van-Leengoed HL, Cuomo V, Versteeg AA et al (1994). In vivo fluorescence and photodynamic activity of zinc phtalocyanine administered in liposomes. British Journal of Cancer 65, 840–845.Google Scholar
  6. 6.
    Pouliquen D, Perroud H, Calza F et al (1992). Investigation of the Magnetic Properties of Iron Oxide Nanoparticles Used as Contrast Agents for MRL Magnetic Resonance in Medicine 24, 75–84.Google Scholar
  7. 7.
    Weissleder R, Elizondo G, Wittenberg J et al (1990). Ultrasmall superparamagnetic iron oxide: characterization of a new class of contrast agents for MR imaging. Radiology 175, 489–493.Google Scholar
  8. 8.
    Bach-Gansmo T, Fahlvik A, Ericsson A et al (1994). Superparamagnetic Iron Oxide for Liver Imaging, Comparison Among Three Different Preparations. Investigative Radiology 29, 339–344.Google Scholar
  9. 9.
    Marincek B (1996). Diagnostic improvement in MRI of gynecological neoplasms. Journal Belge Radiology 79 13–17.Google Scholar
  10. 10.
    Thomas C, Nijenhus AM, Timens W et al (1993). Liver metastasis model of colon cancer in the rat. Immunhistochemical characterization. Invasion-Metastasis 13, 102–112.Google Scholar
  11. 11.
    Buske N, Sonntag H, Götze T (1984). Magnetic fluids–their preparation, stabilization and applications in colloid science. Colloids and surfaces 12, 195–202.CrossRefGoogle Scholar
  12. 12.
    Chambon C, Clement O, LeBlanche A et al (1993). Superparamagnetic iron oxides as positive MR contrast agents: in vitro and in vivo evidence. Magnetic Resonance Imaging 11, 509–519.Google Scholar
  13. 13.
    Hennig J, Nauerth A, Friedburg H (1986). RARE imaging: A fast imaging method for clinical MR. Magnetic Resonance in Medicine 3, 823–833.Google Scholar
  14. 14.
    Gabe M (1976). Histological techniques. Mason et Cie, Paris, 1st Edition, 313.CrossRefGoogle Scholar
  15. 15.
    Allen TM (1994). Long-circulating (sterically stabilized) liposomes for targeted Drug delivery. Tips 15, 215–220.Google Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Sabine Päuser
    • 1
  • Regina Reszka
    • 3
  • Susanne Wagner
    • 1
  • Karl-Jürgen Wolf
    • 1
  • Heinz Johannes Buhr
    • 2
  • Gerd Berger
    • 2
  1. 1.Department of RadiologyGermany
  2. 2.Department of Surgery Universitätsklinikum “Benjamin Franklin”Freie Universität BerlinBerlinGermany
  3. 3.Drug TargetingMax Delbrück Center for Molecular MedicineBerlinGermany

Personalised recommendations