Specific Blood Purification by Means of Antibody-Conjugated Magnetic Microspheres
The employment of immobilized antibodies (antigens) offers the most selective approach to extracorporeal adsorptive removal of humoral factors from blood. Polypeptide mediators and antibodies in systemic inflammatory and autoimmune diseases, respectively, can not be depleted by hemodialysis treatment, and thus represent attractive targets for immunoadsorption regimen. In this article we describe the elimination of interleukin 1β (IL-1β) and tumor necrosis factor α (TNFα), the major pathogenic mediators in septic shock and multi-organ failure, by superparamagnetic microspheres used in the novel MDS blood purification technology. The particles, consisting of antibody conjugates, exhibit high adsorption rate, capacity, selectivity, and biocompatibility. By application in an in vitro MDS set-up, human plasma could be efficiently cleansed from IL-lß and TNFa. Contrary to conventional blood and plasma chromatography techniques, MDS offers a high degree of flexibility and continuous blood processing due to magnetic adsorbent replacement.
KeywordsImmobilize Antibody Therapeutic Plasma Exchange Blood Purification Secondary Circuit High Adsorption Rate
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