Specific Blood Purification by Means of Antibody-Conjugated Magnetic Microspheres

  • Christoph Weber
  • Dieter Falkenhagen
Chapter

Abstract

The employment of immobilized antibodies (antigens) offers the most selective approach to extracorporeal adsorptive removal of humoral factors from blood. Polypeptide mediators and antibodies in systemic inflammatory and autoimmune diseases, respectively, can not be depleted by hemodialysis treatment, and thus represent attractive targets for immunoadsorption regimen. In this article we describe the elimination of interleukin 1β (IL-1β) and tumor necrosis factor α (TNFα), the major pathogenic mediators in septic shock and multi-organ failure, by superparamagnetic microspheres used in the novel MDS blood purification technology. The particles, consisting of antibody conjugates, exhibit high adsorption rate, capacity, selectivity, and biocompatibility. By application in an in vitro MDS set-up, human plasma could be efficiently cleansed from IL-lß and TNFa. Contrary to conventional blood and plasma chromatography techniques, MDS offers a high degree of flexibility and continuous blood processing due to magnetic adsorbent replacement.

Keywords

Immobilize Antibody Therapeutic Plasma Exchange Blood Purification Secondary Circuit High Adsorption Rate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Christoph Weber
    • 1
  • Dieter Falkenhagen
    • 1
  1. 1.Centre for Biomedical TechnologyDonau-Universität KremsKremsAustria

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