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Ferromagnetic and Immunomagnetic Concentration of Mycobacteria for the Diagnosis of Tuberculosis

  • Michael A. Vladimirsky
  • Victor I. Philippov
  • Anatoly A. Kuznetsov
  • Alexander I. Polozov
  • Edward K. Dobrinsky
Chapter

Abstract

A magnetic separation method for the concentration of tuberculosis bacteria and its application for the diagnosis of tuberculosis in clinical trials in Russia is described. Magnetic particles were developed which selectively bind to tuberculosis bacilli. The tuberculosis cells attached to the particles can be extracted by a centrifuge or a magnetic separator. The detection of the bacteria was then possible by either traditional microscopic and microbiological methods or molecular diagnostic techniques (PCR). The magnetic method was almost as sensitive in diagnosing tuberculosis as the much more time and work intensive cultivation method.

Keywords

Cultivation Method Ferromagnetic Particle Sputum Specimen Suspected Tuberculosis Trisodium Phosphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Michael A. Vladimirsky
    • 1
    • 3
  • Victor I. Philippov
    • 3
    • 2
  • Anatoly A. Kuznetsov
    • 1
    • 3
  • Alexander I. Polozov
    • 1
    • 3
  • Edward K. Dobrinsky
    • 3
    • 2
  1. 1.Synmatix CorporationSouthfieldUSA
  2. 2.Russian Research Institute of PhtisiopulmonologyMoscowRussia
  3. 3.Institute of Biochemical PhysicsRussian Academy of SciencesMoscowRussia

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