Properties and Applications of Genetically Engineered Streptavidins
The wide application of magnetic bead technology has been greatly facilitated by the use of streptavidin (avidin)-biotin systems, with which a variety of biological materials can be tightly immobilized on solid surfaces. The streptavidin gene was cloned from Streptomyces avidinii, and it was expressed at high levels in Escherichia coli despite potent antibacterial action of the gene product. The DNA sequence has been modified to provide streptavidin with a number of basic variations in size, stability, and biotin-binding characteristics, and to construct a system that allows the design of multi-functional proteins by making novel gene fusions with the streptavidin gene. These genetically engineered streptavidins are extremely useful for a variety of biotechnological applications, including those with magnetic beads.
KeywordsBiotin Binding Biotin System Tetrameric Molecule Recombinant Core Core Streptavidin
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