Continuous-Flow Magnetic Cell Sorting Using Soluble Immunomagnetic Label

  • Maciej Zborowski
  • Lee R. Moore
  • Liping Sun
  • Jeffrey J. Chalmers


Soluble immunomagnetic labels in application to cell sorting offer potential advantages of binding in proportion to cell surface receptor density, and thus high sensitivity. These advantages could be fully realized in magnetic cell sorting systems based on continuous, steady-state cell separation rather than by cell deposition on magnetic surfaces as it is practiced today. Two continuous-flow magnetic cell sorting systems based on the dipole and the quadrupole magnetic fields were designed, built and tested. The cell model system comprised human peripheral lymphocytes, mouse anti-human cytotoxic T cell monoclonal antibody-fluorescein, and rat anti-mouse immunomagnetic colloid. Changes in the fractional concentration of the cytotoxic T cells before and after magnetic fractionation were measured by flow cytometry. The preliminary results indicated sensitivity of the continuous-flow cell separation following magnetic immunocolloid labeling to population distribution of the cell label.


Cell Separation Particle Tracking Velocimetry Magnetic Cell Quadrupole Field Magnetic Cell Sorting 
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  1. 1.
    Ugelstad J, Stenstad P, Kilaas L, Prestvik WS, Herje R, Berge A, Homes E (1993). Monodispersemagnetic polymer particles. Blood Purif 11, 347–369.Google Scholar
  2. 2.
    Radbruch A, Mechtold B, Thiel A, Miltenyi S, Pflüger E (1994). High-gradient magnetic sorting. Methods in Cell Biology 42, 387–403.CrossRefGoogle Scholar
  3. 3.
    Hancock JP, Kemshead JT (1993). A rapid and highly selective approach to cell separations using an immunomagnetic colloid. J Immunol Meth 164, 51–60.Google Scholar
  4. 4.
    Shapiro HM (1995). Practical Cytometry. Wiley-Liss, New York.Google Scholar
  5. 5.
    Ibid., p.284.Google Scholar
  6. 6.
    Graham MD (1984). Comparison of volume and surface mechanisms for magnetic filtration of blood cells. J Phys Colloq. 45 (Suppl au no.1), C1:779-C1:784.Google Scholar
  7. 7.
    Winoto-Morbach S, Tchikov V, Müller-Ruchholtz (1994). Magnetophoresis: I. Detection of magnetically-labeled cells. J Clin Lab Anal 8, 400–406.Google Scholar
  8. 8.
    Gee AP (1994). Immunologically based methods for the elimination of tumor cells from autologous stem cell grafts. Immunomethods 5 232–242.CrossRefGoogle Scholar
  9. 9.
    Liberti PA, Feeley BP (1991). Analytical-and process-scale cell separation with bioreceptor ferrofluids and high gradient magnetic separation. In Cell separation Science and Technology. Compaia DS and Todd P (Eds), ACS Symposium Series, Washington, 464 268–288.Google Scholar
  10. 10.
    Zborowski M, Fuh CB, Green R, Sun L, Chalmers JJ (1995). Analytical magnetapheresis offerritin-labeled lymphocytes. Anal Chem 67, 3702–3712.CrossRefGoogle Scholar
  11. 11.
    Zborowski M, Fuh CB, Green R, Baldwin NJ, Reddy S, Douglas T, Mann S, Chalmers JJ (1996). Immunomagnetic isolation of magnetoferritin-labeled cells in a modified ferrograph. Cytometry 24, 251–259.Google Scholar
  12. 12.
    Oberteuffer JA (1973). High gradient magnetic separation. IEEE Trans Mag MAG-9 303–306Google Scholar
  13. 13.
    Watson JHP (1990). High gradient magnetic separation. In Solid-liquid Separation, Svarovsky L (Ed), Butterworths, London, 661–684.Google Scholar
  14. 14.
    Lewis D, Wellington TD (1976). Some old and new concepts in magnetic separation. IEEE Trans MAG-12 480–485.Google Scholar
  15. 15.
    Sun JJ (1980). Methods and apparatus for separating particles using a magnetic barrier. US Patent 4,235,710.Google Scholar
  16. 16.
    Hwang JY, Takayasu M, Friedlaender FJ, Kullerud (1984). Application of magnetic susceptibility gradients to magnetic separation. J Appl Phys 55, 2592–2594.Google Scholar
  17. 17.
    Takayasu M, Kelland DR (1986). Selective continuous magnetic separation of two-component particulate suspensions. IEEE Trans MAG-22 1125–1127.ADSGoogle Scholar
  18. 18.
    Doctor RD, Panchal CB, Swietlik CE (1986). A model of open-gradient magnetic separation for coal cleaning using a superconducting quadrupole field. AIChE Symposium Series “Recent Advances in Separation Techniques–III” 82, 154–168.Google Scholar
  19. 19.
    Vanderhoff JW, Micale FJ, Krumrine PH (1979). Continuous flow electrophoresis. In Electrokinetic Separation Methods, Righetti PG, van Oss CJ, VanderhoffJW (Eds), Elsevier/North Holland Biomedical Press, New York, 121–141.Google Scholar
  20. 20.
    Hannig K (1982). New aspects in preparative and analytical continuuous free flow cell electrophoresis. Electrophoresis 3 235–243.Google Scholar
  21. 21.
    Mattock P, Aitchison GF, Thomson AR (1980). Velocity gradient stabilised, continuous, free flow electrophoresis. A review. Sep and Purif Meth 9, 1–68.Google Scholar
  22. 22.
    Weber E (1960). Electromagnetic Fields. Theory and Applications. Vol. 1 - Mapping of Fields. John Wiley and Sons, Inc., New York, 345.Google Scholar
  23. 23.
    Dawson PH (1976). Quadrupole Mass Spectrometry and Its Applications. Elsevier Scientific Publishing Company, New York.Google Scholar
  24. 24.
    Guezenec YG, Brodkey RS, Trigui N, Kent JC (1994). Algorithms for fully-automated three-dimensional particle tracking velocimety. Exp Fluids 17, 209–219.Google Scholar
  25. 25.
    Reddy S, Moore LR, Sun L, Zborowski M, Chalmers JJ (1996). Determination of the magnetic susceptibility of labeled particles by video imaging. Chemical Engineering Science 51, 947–956.Google Scholar
  26. 26.
    Hartig R, Hausman M, Schmitt J, Herrmann DBJ, Riedmeiller M, Cremer C (1992). Preparative continuous separation of biological particles by means offree-flow magnetophoresis in a free flow electrophoresis chamber. Electrophoresis 13, 674–676.Google Scholar
  27. 27.
    Hartig R, Hausmann M, Cremer C (1995.) Continuous focusing of biological particles by continuous immunomagnetic sorter: technique and applications. Electrophoresis 16, 789–792.CrossRefGoogle Scholar
  28. 28.
    Thomas TE, Abraham SJR, Otter AJ, Blackmore EW, Lansdorp PM (1992). High gradient magnetic separation of cells on the basis of expression levels of cell surface antigens. J Immunol Meth 154, 245–252.Google Scholar
  29. 29.
    Frantz SG (1936). Magnetic separation method and means. US Patent 2,056,426.Google Scholar
  30. 30.
    Zborowski M, Williams PS, Sun L, Moore LR, Chalmers JJ (1997). Cylindrical SPLITT and quadrupole magnetic field in application to continuous flow magnetic cell sorting. J Liquid Chromat Related Tech (in print).Google Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Maciej Zborowski
    • 1
  • Lee R. Moore
    • 1
  • Liping Sun
    • 2
  • Jeffrey J. Chalmers
    • 2
  1. 1.Department of Biomedical Engineering/Wb-3The Cleveland Clinic FoundationClevelandUSA
  2. 2.Department of Chemical EngineeringThe Ohio State UniversityColumbusUSA

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