Analysis of Microspheres in Living Cells by Confocal Microscopy

  • Stine-Kathrein Kraeft
  • Urs O. Häfeli
  • Lan Bo Chen


Confocal laser scanning microscopy is a versatile tool to study small particles in biological tissues. Optical sectioning allows the detection and localization of particles for various biomedical applications, at a single cell level or within a complex tissue. Most applications with particles employ fluorescent particles, and the example of fluorescein-conjugated latex beads as model substrates to study antigen processing in B-lymphocytes is discussed. Detection of particles inside intact B cells clearly demonstrated internalization of the antigen during antigen presentation. Confocal microscopy also allows the analysis of the internal morphology of magnetic microspheres (MMS). Such microspheres designed for targeted radiotherapy were studied together with living or fixed cells using multi-parameter imaging in epi-fluorescent, reflection and transmission modes. The results demonstrate that confocal microscopy is a valid tool to directly analyze the internal composition of magnetic carrier systems as well as their distribution within complex biological tissues.


Confocal Microscopy Transmission Mode Optical Section Reflection Mode Latex Bead 
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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Stine-Kathrein Kraeft
    • 1
    • 3
  • Urs O. Häfeli
    • 2
  • Lan Bo Chen
    • 1
    • 3
  1. 1.Dana-Farber Cancer InstituteHarvard Medical SchoolBostonUSA
  2. 2.Radiation Oncology DepartmentThe Cleveland Clinic FoundationClevelandUSA
  3. 3.Institute of Clinical Chemistry and PathobiochemistryUniversity of RostockRostockGermany

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