Development and Validation of Multiwavelength Algorithms for in Vivo Near Infrared Spectroscopy
The ability to measure changes in tissue oxygenation noninvasively is of potential benefit to managing a variety of problems in clinical medicine. One useful way to assess tissue oxygenation in situ. is to measure oxygen-dependent light absorption by hemoglobin and other chromophores present in the tissues. Soft tissues and bone are relatively translucent to near infrared (NIR) photons and three important biological molecules, hemoglobin (Hb+Hb02), myoglobin (Mb+Mb02) and oxidized cytochrome c oxidase (cyt a, a.3) have oxygen-dependent absorption spectra in the 700–900 nm wavelength region. Therefore, NIR spectroscopy offers a potential solution to the problem of noninvasive measurement of changes in tissue oxygenation. These concepts were first reported from Duke University in 1977 when Jöbsis1 demonstrated a “window” for transmission of NIR light in biological tissues and the feasibility of in vivo NIR monitoring of changes in the oxygenation state of hemoglobin and the oxidation level of cyt a, a3 in the brain. Continuous, noninvasive NIR monitoring techniques to evaluate tissue oxygenation have evolved gradually over the past 10 years at Duke and other institutions2–4.
KeywordsCerebral Oxygenation Near Infrared Spectroscopy Exchange Transfusion Blood Flow Restriction Chronic Venous Insufficiency
Unable to display preview. Download preview PDF.
- 2.I. Giannini, M. Ferrari, A. Carpi and P. Fasella, Non-invasive near infrared spectroscopy of brain and fluorocarbon exchange transfused rats, Physiol. Chem. Phys., 14: 295305 (1982).Google Scholar
- 3.O. Hazeki, A. Seiyama and M. Tamura, Near infrared spectrophotometric monitoring of hemoglobin and cytochrome a,a3 in situ jn: “Advances in Experimental Medicine and Biology,” A. Silver and A. Silver, eds., Plenum Press, New York (1986).Google Scholar
- 7.F.F. Jöbsis-VanderVliet, Non-invasive, near infrared monitoring of cellular oxygen sufficiency jn vivo jj.: “Advances in Experimental Medicine and Biology,” F. Kreuzer, S. M. Cain, Z. Turek and T. K. Goldstick, eds., Plenum Press, New York (1985).Google Scholar
- 10.H.H. Keizer, F. F. Jöbsis-VanderVliet, S. K. Lucas, C. A. Piantadosi and A. L. Sylvia, The near infrared absorption band of cytochrome a,$3 in purified enzyme, isolated mitochondria and in the intact brain jn situ in: “Advances in Experimental Medicine and Biology,” F. Kreuzer, S. M. Cain, Z. Turek and T. K. Goldstick, eds., Plenum Press, New York (1985).Google Scholar
- 12.H.J. Proctor, C. Cairns, D. Fillipo and F.F. Jöbsis-VanderVliet, Near infrared spectrophotometry: potential role during increased intracranial pressure, in: “Advances in Experimental Medicine and Biology,” F. Kreuzer, S. M. Cain, Z. Turek and T. K. Goldstick, eds., Plenum Press, New York (1985).Google Scholar
- 14.F.F. Jöbsis-VanderVliet, E. Fox and K. Sugioka, Monitoring of cerebral oxygenation and cytochrome $,33 redox state, ja: “International Anesthesiology Clinics,” K.K. Tremper, ed., Little, Brown and Co., Boston (1987).Google Scholar
- 17.D.H. Glaister, Current and emerging technology in G-LOC detection: Non-invasive monitoring of cerebral microcirculation using near infrared, Aviat., Space, Environ. Med., 59: 23–28 (1988).Google Scholar
- 18.J.A. Griebel, N.B. Hampson and C.A. Piantadosi, Cytochrome redox states correlated with 02 uptake and oleic acid lung injury in rabbits, Am. Rev. Resp. Dis ., 137: 115A (1988).Google Scholar
- 19.N.B. Hampson, E.M. Camporesi, R.E. Moon, B.W. Stolp, J.A. Griebel, S.L. Whitney and C.A. Piantadosi, Effects of hypocapnic and normocapnic hypoxia on cerebral oxygenation and ventilatory responses in humans, Am. Rev. Resp. Dis ., 137: 143A (1988)Google Scholar
- 20.C.A. Piantadosi, J.A. Griebel and N.B. Hampson, Intramitochondrial oxygenation decreases in forearm muscle during venous congestion, Clin. Res., 36: 373A (1988).Google Scholar