Some Prospects for Adapting Fluorescence Instrumentation

  • Jay R. Knutson


We can classify the information content of light which is emitted, reflected or scattered by tissue as four types: 1, direction of propagation; 2, polarization state; 3, energy (wavelength) and 4, time of detection. Unfortunately, the first two are rather quickly “scrambled” in turbid media. Scattering also broadens energy lines and disperses path length; hence all of these may be degraded.


Turbid Medium Timing Grid Decay Profile Time Resolve Fluorescence Spectroscopy Mesh Simulation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    L. Brand, J.R. Knutson, L. Davenport, J.M. Beechem, R.E. Dale, D.G. Walbridge, A.A. Kowalczyk, Time resolved fluorescence spectroscopy: Some applications of associative behaviour to studies of proteins and membranes, im “Spectroscopy and the Dynamics of Molecular Biological Systems,” P.M. Bayley, R.E. Dale, eds., Academic, London (1985).Google Scholar
  2. 2.
    J. R. Knutson, L. Davenport, J.M. Beechem, D.G. Walbridge, M. Ameloot, L. Brand, Associated spectra and the multidimensional nature of fluorescence spectroscopy, im “Excited-State Probes in Biology and Medicine,” A. Szabo and L. Masotti, eds., Plenum, New York (in press).Google Scholar
  3. 3.
    R.P. Van Duyne, D.L. Jeanmaire, D.F. Shriver, Mode locked laser raman spectroscopy - A new technique for the rejection of interfering background luminescence signals, Anal.Chem., 46: 213 (1974).CrossRefGoogle Scholar
  4. 4.
    J.M. Harris, R.W. Chrisman, F.E. Lytle, R.S. Tobias, Sub-nanosecond time-resolved rejection of fluorescence from raman spectra, Anal. Chem., 48: 1937 (1976).CrossRefGoogle Scholar
  5. 5.
    J.R. Knutson, Time-resolved laser spectroscopy in biochemistry, fluorescence detection: Schemes to combine speed, sensitivity and spatial resolution, J. Lakowicz, ed., SPIE Vol. 909, Bellingham (1988).Google Scholar
  6. 6.
    J.R. Knutson, D.G. Walbridge and L. Brand, Decay-associated fluorescence spectra and the heterogeneous emission of alcohol dehydrogenase, Biochemistry 21: 4671 (1982).PubMedCrossRefGoogle Scholar
  7. 7.
    Hamamatsu literature “Applications of Multichannel Plates” and pers.comm. from D. Fatlowitz.Google Scholar
  8. 8.
    G. Holtom, this volume and pers.comm.Google Scholar
  9. 9.
    W.G. McMullan, S. Charbonneau, M.L.W. Thewalt, Simultaneous subnanosecond timing information and 20 spatial information from imaging photomultiplier tubes, Rev.Sci.lnst . 58 (9): 1626–1628 (1987).CrossRefGoogle Scholar
  10. 10.
    H. Leelavathi, J.P. Pichamuthu, Propagation of optical pulses through dense scattering media, Appl.Opt ., 27 (12): 2461–2468 (1988).PubMedCrossRefGoogle Scholar
  11. 11.
    J. Mathews, R.L. Walker, “Mathematical Methods of Physics,” W.A.Benjamin, New York (1970).Google Scholar
  12. 12.
    J.R. Knutson, J.M. Beechem, L. Brand, Simultaneous analysis of multiple fluorescence decay curves: A global approach, Chem.Phys.Lttr ., 102 (6): 501–507 (1983).CrossRefGoogle Scholar
  13. 13.
    J.M. Beechem, M. Ameloot, J.R. Knutson, L. Brand, The global analysis of fluorescence intensity and anisotropy decay data: Second generation theory and programs, in: “Fluorescence Spectroscopy, Vol.!, Principles and Techniques,” J.Lakowicz, ed., Plenum, New York (in press).Google Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Jay R. Knutson
    • 1
  1. 1.Laboratory of Technical DevelopmentNational Heart, Lung and Blood InstituteBethesdaUSA

Personalised recommendations