Abstract
Recent experiments involving light scattering and diffusion in tissues show a substantial signal emerging from large tissue masses on the order of 2–10 nanoseconds (ns) following light entry. A quantitative method for resolving the scattered light should have these attributes:
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a)
High sensitivity. This implies a quantum limited detector such as a photomultiplier tube (PMT).
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b)
Good dynamic range, ideally five decades if needed.
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c)
Stable, linear, and adjustable time sweep for convenient comparison of data to theoretical models.
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References
Lewis, C., W. R. Ware, L. J. Doemeny and T. L. Nemzek, The measurement of short-lived fluorescence decay using the single photon counting method, Rev. Sci. Instr., 44: 107114, (1973).
Spears, K. G., L. E. Cramer and L. D. Hoffland, Subnanosecond time-correlated photon counting with tunable lasers, Rev. Sci. Instr., 49: 255–262, (1978).
Kume, H., K. Nakatusgawa, S. Suzuki and D. Fatlowitz, Ultrafast microchannel plate photomultipliers, Appl. Opt., 27: 1170–1178, (1988).
D. Fatlowitz, personal communication.
O’Connor, D. V., W. R. Ware and J. C. Andre, Deconvolution of fluorescence decay curves, a critical comparison of techniques, J. Phys. Chem., 83: 1333, (1979).
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© 1989 Springer Science+Business Media New York
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Holtom, G.R. (1989). Experimental Time Resolved Methods. In: Chance, B. (eds) Photon Migration in Tissues. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-6178-8_11
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DOI: https://doi.org/10.1007/978-1-4757-6178-8_11
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4419-3215-0
Online ISBN: 978-1-4757-6178-8
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