Oxygen Transport to Tissue XXV pp 201-206 | Cite as
Haemoglobin-Enhanced Mitosis in Cultured Plant Protoplasts
Abstract
Potato (Solanum tuberosum) is the world’s fourth most economically important food crop, following wheat, rice and maize. Potato breeding is slow compared to that of other major crops, making it an important candidate for in vitro genetic manipulation involving direct gene transfer into isolated protoplasts (‘naked cells’, from which the walls have been removed by enzymatic digestion), and somatic hybridisation or cybridisation. Both of the latter techniques are based on the fusion of protoplasts from potato with those of other Solanum species. A pre-requisite for such investigations is reproducible growth in culture of protoplasts to cells, followed by the differentiation of protoplast-derived tissues into fertile plants. An adequate and sustainable oxygen supply is a fundamental requirement to maximise growth of protoplasts and protoplast-derived cells. Previous studies have demonstrated the beneficial effects of medium supplementation with the chemically-modified haemoglobin (Hb), Erythrogen TM,on mitotic division of protoplastderived cells and subsequent plant regeneration in Oryza sativa (Azhakanandam et al., 1997; Al-Forkan et al., 2001), Passiflora giberti and Petunia hybrida cv. Comanche (Anthony et al., 1997). However, there have been no corresponding studies with major dicotyledonous crops, such as potato.
Keywords
Mitotic Division Cool White Fluorescent Tube Cotton Cell Subsequent Plant Regeneration Settle Cell VolumePreview
Unable to display preview. Download preview PDF.
References
- Al-Forkan, M.A., Anthony, P., Power, J.B., Davey, M.R., and Lowe, K.C., 2001, Haemoglobin (Erythrogen TM )_ enhanced microcallus formation from protoplasts of Indica rice (Oryza saliva L.). Art. Cells, Blood Subs., Immob. BiotechnoL 29: 399–404.Google Scholar
- Anthony, P., Davey, M.R., Power, J.B., and Lowe, K.C., 1997, Enhanced mitotic division of cultured Passjora and Petunia protoplasts by oxygenated perfluorocarbon and haemoglobin. Biotechnol. Tech. 11: 581–584.CrossRefGoogle Scholar
- Azhakanandam, K., Lowe, K.C., Power, J.B., and Davey, M.R., 1997, Haemoglobin (Erythrogen“)-enhanced mitotic division and plant regeneration from cultured rice protoplasts (Oryza saliva L.). Enzyme Microb. Technol. 21: 572–577.CrossRefGoogle Scholar
- Cheng, J.P., Saunders, J.A., and Sinden, S.L., 1995, Colorado potato beetle resistant somatic hybrid potato plants produced via protoplast electrofusion. In Vitro Cell. Dev. Biol. — Plant 31: 90–95.CrossRefGoogle Scholar
- Garratt, L.C., Anthony, P., Power, J.B., Davey, M.R., and Lowe, K.C., 2003, Growth and antioxidant status of plant cells cultured with bovine haemoglobin solution. Oxygen Transport to Tissue, Vol. XXV, Kluwer Academic/Plenum Publishers, New York, this volume.Google Scholar
- Kapur, R., Saleem, M., Harvey, B.L., and Cutler, A.J., 1993, Oxidative metabolism and protoplast culture. In Vitro Cell. Dev. BioL — Plant 29: 200–206.CrossRefGoogle Scholar
- Kumar, A., 1994, Agrobacterium-mediated transformation of potato genotypes. in: Agrobacterium Protocols, Methods in Molecular Biology’, Vol. 44, K. Gartland, and M.R. Davey, eds., Humana Press, Totowa, pp. 121–128.Google Scholar
- Shepard, J.F., 1980, Mutant selection and plant regeneration from potato mesophyll protoplasts. in: Genetic Improvement of Crops — Emergent Techniques. I. Rubenstein, B. Gengenbach, R.L. Phillips, and C.E. Green, eds., University of Minnesota Press, Minneapolis, pp. 185–219.Google Scholar
- Snedecor, G.W., and Cochran, W.G., 1989, Statistical Methods, 8th edn., Iowa State College Press, Ames. Uchimiya, H., and Murashige, T., 1974, Evaluation of parameters in the isolation of viable protoplasts from cultured tobacco cells. Plant Physiol. 54: 936–944.Google Scholar