Analysis of Equilibrium Adsorption Isotherms for Human Protein C Purification by Immobilized Metal Affinity Chromatography

  • Renu Nandakumar
  • Hessam Afshari
  • Duane F. Bruley
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 540)


Protein C (PC) is a glycoprotein that plays a pivotal role as an anticoagulant, antithromobotic, and thrombolytic therapeutic in the blood coagulation cascade (Esmon, 1989). It is a member of the vitamin K-dependent (VKD) family also consisting of coagulation protein factors VII, IX, X, proteins S, Z and prothrombin. Human PC is synthesized in the liver as a single chain precursor and circulates in the blood primarily as a two chain zymogen that is activated by proteolytic clevage. Activated PC is a potent serine protease that regulates blood coagulation by deactivating factors Va and VIIIa (a: activated form), thus preventing generation of the enzyme factors Xa and thrombin (Wu and Bruley, 1999).


Deep Vein Thrombosis Immobilize Metal Affinity Chromatography Equilibrium Adsorption Isotherm Blood Coagulation Cascade Maryland Baltimore County 
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Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Renu Nandakumar
  • Hessam Afshari
  • Duane F. Bruley
    • 1
  1. 1.College of EngineeringUniversity of Maryland Baltimore CountyBaltimoreUSA

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