Growth and Antioxidant Status of Plant Cells Cultured with Bovine Haemoglobin Solution

  • Lee C. Garratt
  • Paul Anthony
  • J. Brian Power
  • Michael R. Davey
  • Kenneth C. Lowe
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 540)


Reactive oxygen species (ROS), such as superoxide (O2 •-), hydrogen peroxide (H2O2), and the hydroxyl radical (•OH), may be generated in plant cells during aerobic metabolism. ROS can impair cell activities, primarily through oxidative damage to lipids, proteins and nucleic acids (Halliwell and Gutteridge, 1999). Exposure of plants or their cells/tissues, maintained in vitro, to environmental stresses (e.g. temperature extremes, drought, high salinity, mineral deficiency) perturbs the balance between the ROS production and the quenching effects of antioxidant enzymes, leading to oxidative damage (Smirnoff, 1993; Foyer and Mullineaux, 1994; Schwanz et al., 1996). However, plants/cells possessing high activities of constitutive or induced antioxidant enzymes show increased resistance to such oxidative damage (Halliwell and Gutteridge, 1999; Niki, 2000).


Antioxidant Status Mitotic Division Callus Induction Medium Cellular Reactive Oxygen Species Plant Cell Growth 
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  1. Adkins, S.W., 1992, Cereal callus cultures: Control of headspace gases can optimise the conditions for callus proliferation. Aust. J. Bot. 40: 737–749.CrossRefGoogle Scholar
  2. Adkins, S.W., Kunanuvatchaidach, R., Gray, S.J., and Adkins, A.L., 1993, Effect of ethylene and culture environment on rice callus proliferation. J. Exp. Bot. 44: 1829–1835.CrossRefGoogle Scholar
  3. Aebi, H., 1984, Catalase in vitro. Methods Enzymol. 105: 121–126.CrossRefGoogle Scholar
  4. Anthony, P., Lowe, K.C., Power, J.B., and Davey, M.R., 1997, Strategies for promoting division of cultured plant protoplasts: synergistic beneficial effects of haemoglobin (ErythrogenTM) and Pluronic ® F-68. Plant Cell Rep. 17: 13–16.CrossRefGoogle Scholar
  5. Azhakanandam, K., Lowe, K.C., Power, J.B., and Davey, M.R., 1997, Hemoglobin (ErythrogenTM)-enhanced mitotic division and plant regeneration from cultured rice protoplasts (Oryza sativa L.). Enzyme Microb. Technol. 21: 572–577.CrossRefGoogle Scholar
  6. Alayash, A.L., 2000, Hemoglobin-based blood substitutes and hazards of blood radicals. Free Radic. Res. 33: 34 1348.Google Scholar
  7. Beauchamp, C.O., and Fridovich, I., 1971, Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44: 276–287.PubMedCrossRefGoogle Scholar
  8. Benson, E.E., Magill, W.J., and Bremner, D.H., 1997, Free radical processes in plant tissue cultures: implications for plant biotechnology programmes. Phyton 37: 31–38.Google Scholar
  9. Benson, E.E., 2000, Do free radicals have a role in plant tissue recalcitrance? In Vitro Cell. Dev. Biol.–Plant 36: 163–170.CrossRefGoogle Scholar
  10. Bradford, M.M., 1976, A rapid and sensitive method for the quantification of microgram quantities of protein utilising the principle of protein-dye binding. Anal. Biochem. 72: 248–254.PubMedCrossRefGoogle Scholar
  11. Foyer, C.H., and Mullineaux, P.M., 1994, Causes of photooxidative stress and amelioration of defence systems in plants. CRC Press, Florida.Google Scholar
  12. Guilbault, G.G., Kramer, D.N., and Hackley, E., 1967, A new substrate for fluorimetric determination of oxidative enzymes. Anal. Chem. 39: 271.CrossRefGoogle Scholar
  13. Halliwell, B., and Gutteridge, J.M.C., 1999, Free radicals in biology and medicine, 3rd edn., Oxford University Press, Oxford.Google Scholar
  14. Huang, S.Y., and Chou, C.J., 2000, Effect of gaseous composition on cell growth and secondary metabolite production in suspension cultures of Stizolobium hassjoo cells. Bioprocess Eng. 23: 585–593.CrossRefGoogle Scholar
  15. Jordi, W., Stoopen, G.M., Argiroudi, I., Veld, E., Heinen, P., and van Tol, H., 1996, Accumulation of a 50-kD protein during leaf senescence of Alstroemeria cut flowering stems. Physiol. Plant. 98: 819–823.CrossRefGoogle Scholar
  16. McCord, J.M., and Fridovich, I., 1969, Superoxide dismutase: An enzymatic function for erythrocuprein (hemocuprein). Biol. Chem. 244: 6049–6055.Google Scholar
  17. Murashige, T., and Skoog, F., 1962, A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 56: 473–497.CrossRefGoogle Scholar
  18. Niki, E., 2000, Action and role of antioxidants against oxidative stress. J. Japan Soc. Biosci. Biotechnol. Agrochem. 74: 799–801.Google Scholar
  19. Schwanz, P., Picon, C., Vivin, P., Dreyer, E., Guehi, J.M., and Polle, A., 1996, Responses of antioxidative systems to drought stress in pendunculate oak and maritime pine as modulated by elevated CO2. Plant Physiol. 110: 393–402.PubMedGoogle Scholar
  20. Sen-Gupta, A., Webb, R.P., Holaday, A.S., and Allen, R.D., 1993, Overexpression of superoxide dismutase protects plants from oxidative stress. Plant Physiol. 103: 1067–1073.Google Scholar
  21. Slooten, L., Caipau, K., van Camp, W., van Montagu, M., Sybesma, C., and Inzé, D., 1995, Factors affecting the enhancement of oxidative stress tolerance in transgenic tobacco overexpressing manganese superoxidedismutase in the chloroplasts. Plant Physiol. 107: 737–750.PubMedGoogle Scholar
  22. Smimoff, N., 1993, The role of active oxygen in the response of plants to water deficit and desiccation. New Phytol. 125: 27–58.CrossRefGoogle Scholar
  23. Snedecor, G.W., and Cochran, W.G., 1989, Statistical Methods, 8th edn., Iowa State College Press, Ames.Google Scholar

Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Lee C. Garratt
    • 1
  • Paul Anthony
    • 1
  • J. Brian Power
    • 1
  • Michael R. Davey
    • 1
  • Kenneth C. Lowe
    • 2
  1. 1.School of BiosciencesUniversity of NottinghamLoughboroughUK
  2. 2.School of Life & Environmental SciencesUniversity of NottinghamNottinghamUK

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