Applications and Benefits of a Non-Ionic Surfactant and Artificial Oxygen Carriers for Enhancing Post-Thaw Recovery of Plant Cells from Cryopreservation

  • Michael R. Davey
  • Paul Anthony
  • J. Brian Power
  • Kenneth C. Lowe
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 540)


Embryogenic (totipotent) cells cultured in suspension that are capable of regenerating into intact fertile plants, are a routine source for the enzymatic isolation of plant protoplasts (wall-less cells) that are exploited in genetic manipulation studies, particularly for cereals, including rice (Kinoshita and Mori, 2001). Such suspensions are also an alternative source to immature zygotic embryos for transgenic plant production by biolistics (van Schaik et al., 2000). However, the establishment and maintenance of embryogenic suspensions is technically difficult, since for example, morphogenic competence declines progressively with culture at physiologically normal temperatures (Pradhan et al., 1998). Cryopreservation is exploited for the stable, long-term storage of biological tissues at ultra-low temperatures (Moukadiri et al., 2002), negating the requirement to re-initiate and characterize new cell lines to provide a constant supply of competent cells (Lynch et al., 1994). The recovery of frozen cells depends upon prefreeze, cryogenic and post-freeze conditions. However, the transition of cells between ultra-low and physiologically normal temperatures can induce respiratory imbalances, leading to the production of toxic oxygen radicals (Cella et al., 1982; Benson et al., 1992, 1995).


Rice Cell Immature Zygotic Embryo Rice Protoplast Toxic Oxygen Radical Artificial Oxygen Carrier 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Michael R. Davey
    • 1
  • Paul Anthony
    • 1
  • J. Brian Power
    • 1
  • Kenneth C. Lowe
    • 2
  1. 1.School of BiosciencesUniversity of Nottingham, Sutton Bonington CampusLoughboroughUK
  2. 2.School of Life & Environmental SciencesUniversity of NottinghamNottinghamUK

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