Abstract
Oxygen is an essential metabolite and alterations in tissue oxygen levels cause or contribute to many pathophysiological states. An effective method to non-invasively measure the oxygen concentration in biological systems based on oxygen-dependent quenching of phosphorescence (Vanderkooi et al., 1987; Wilson et al., 1988). This method can provide rapid and accurate measurements of tissue oxygen levels at particular points or two dimensional maps of the oxygen distribution in tissue in vivo (Rumsey et al, 1988; Wilson et al., 1992; Vinogradov et al., 1996). Although near infrared phosphors can provide measurements through cm depths of tissue, there remains a need to be able to selectively measure oxygen at specific sites within the tissue, particularly in deeply embedded regions of tissue that cannot be measured from the surface.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Alcala, J. R., Yu, C., and Yeh, G J., 1993, Digital phosphorimeter with frequency domain signal processing: application to real-time fiber-optic oxygen sensing, Rev. Sci. Instrum. 64: 1554.
Gewehr, P. M., and Delpy, D. T., 1993, Optical oxygen sensor based on phosphorescence lifetime quenching and employing a polymer immobilized metalloporphyrin probe, Med. Biol. Eng. Comp. 31: 2.
Horowitz, P., and Hill, W., 1989, The Art of Electronics, Cambridge University Press, pp. 641,669, 729, 899, 1031.
Lakowicz, J. R., 1999, Principles of the Fluorescence Spectroscopy, Kluwer Academic/Plenum Plublishers, New York.
Lo, L.-W., Koch, C. J., and Wilson, D. F., 1996, Calibration of oxygen-dependent quenching of the phosphorescence of Pd-meso-tetra-(4-carboxyphenyl) porphyrin: a phosphor with general application for measuring oxygen concentration in biological systems, Anal. Biochem. 236: 153.
Pastuszko, A.,Lajevardi, N. S., Chen, J., Tammela, O., Wilson, D.F., and Delivoria-Papadopoulos, M., 1993, The effects of graded levels of tissue oxygen pressure on dopamine metabolism in the striatum of newborn piglets, J. Neurochem. 60: 161.
Pawlowski, M., and Wilson, D. F., 1992, Monitoring of the oxygen pressure in the blood of live animals using the oxygen dependent quenching of phosphorescence, Adv. Exp. Med. Biol. 316: 179.
Rumsey, W. L., Vanderkooi, J. M., and Wilson, D. F., 1988, Imaging of phosphorescence: a novel method for measuring oxygen distribution in perfused tissue, Science 241: 1649.
Vanderkooi, J. M., Maniara, G, Green, T. J., and Wilson, D. F., 1987, An optical method for measurement of dioxygen concentration based on quenching of phosphorescence, J. Biol. Chem. 262: 5476.
Vinogradov, S. A., and Wilson, D. F., 1994, Phosphorescence lifetime analysis with a quadratic programming algorithm for determining quencher distributions in heterogenous systems, Biophys. J. 67: 2048.
Vinogradov, S. A., Lo, L.-W., Jenkins, W. T., Evans, S. M., Koch, C., and Wilson, D. F., 1996, Noninvasive imaging of the distribution in oxygen in tissue in vivo using near-infrared phosphors, Biophys. J. 70: 1609.
Vinogradov, S. A., Femandez-Seara, M. A., Dugan, B. W., and Wilson, D. F., 2002, A method for measuring oxygen distribution in tissue using frequency domain phosphorometry, Compar. Biochem. Physiol. A. 132: 147.
Wilson, D. F., Rumsey, W. L., Green, T. J., and Vanderkooi, J. M., 1988, The oxygen dependence of mitochondrial oxidative phoshorylation measured by a new optical method for measuring oxygen, J. Biol. Chem. 263: 2712.
Wilson, D. F., and Cemiglia, G J., 1992, Localization of tumors and evaluation of their state of oxygenation by phosphorescence imaging, Cancer Res. 52: 3988.
Wilson, D. F., Vinogradov, S. A., Dugan, B. W., Biruski, D., Waldron, L., and Evans, S. A., 2002, Measurement of tumor oxygenation using new frequency domain phosphorometers, Compar. Biochem. Physiol. A. 132: 153.
Zankowsky, D., 1996, How to select low-level signals from noise, Laser Focus World 32 (10): 135.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Springer Science+Business Media New York
About this paper
Cite this paper
Lo, LW., Wilson, D.F. (2003). A Micro-Light Guide System for Measuring Oxygen by Phosphorescence Quenching. In: Thorniley, M., Harrison, D.K., James, P.E. (eds) Oxygen Transport to Tissue XXV. Advances in Experimental Medicine and Biology, vol 540. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-6125-2_17
Download citation
DOI: https://doi.org/10.1007/978-1-4757-6125-2_17
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4419-3428-4
Online ISBN: 978-1-4757-6125-2
eBook Packages: Springer Book Archive