Mineral Induction by the Soluble Matrix from Molluscan Shells

  • Ed M. Greenfield
  • Miles A. Crenshaw


Whole matrix (a soluble/insoluble complex) was prepared from Nautilus septal nacre. Spherules formed on the whole matrix in solutions with activities resembling extrapallial fluid. Tripling the calcium in the incubation solution resulted in the formation of blade-like and plate-like crystals on the whole matrix. Mineral did not form on insoluble matrix even in the triple calcium incubation solution. Thus, the soluble matrix appears necessary to induce mineral. In contrast, a number of investigators have shown that the soluble matrix, when it is free in solution, binds to growing crystals and inhibits further growth. Accordingly, we conclude that the soluble matrix must be immobilized in order to induce mineralization.


Amorphous Calcium Phosphate Oyster Shell Incubation Solution Scallop Shell Soluble Matrix 
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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Ed M. Greenfield
    • 1
  • Miles A. Crenshaw
    • 1
  1. 1.Marine Science Curriculum and Dental Research CenterUniversity of North CarolinaChapel HillUSA

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