Abstract
Whole matrix (a soluble/insoluble complex) was prepared from Nautilus septal nacre. Spherules formed on the whole matrix in solutions with activities resembling extrapallial fluid. Tripling the calcium in the incubation solution resulted in the formation of blade-like and plate-like crystals on the whole matrix. Mineral did not form on insoluble matrix even in the triple calcium incubation solution. Thus, the soluble matrix appears necessary to induce mineral. In contrast, a number of investigators have shown that the soluble matrix, when it is free in solution, binds to growing crystals and inhibits further growth. Accordingly, we conclude that the soluble matrix must be immobilized in order to induce mineralization.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
ADDADI, L. and WEINER, S., 1985. Interactions between acidic proteins and crystals: stereochemical requirements in biomineralization. Proc. Nat. Acad. Sci. U.S.A., 82:4110–4114.
BERNHARDT, A.M., MANYAK, D.M. and WILBUR, KM., 1985. In vitro recalcification of organic matrix of scallop shell and serpulid tubes. J. Moll. Stud., 51:284–289.
BLUMENTHAL, N.C., POSNER, A.S. and HOLMES, J.M., 1972. Effect of preparation conditions on the properties and transformation of amorphous calcium phosphate. Mat. Res. Bull., 7:1181–1190.
CRENSHAW, M.A., 1972a. The soluble matrix from Mercenaria mercenaria shell. Biomineralization, 6:6–11.
CRENSHAW, M.A., 1972b. The inorganic composition of molluscan extrapallial fluid. Biol. Bull., Mar. Biol. Lab., Woods Hole, Mass., 143:506–512.
CRENSHAW, M.A., 1982. Mechanisms of normal biological mineralization of calcium carbonates. In Biological Mineralization and Demineralization, (ed. G.H. Nancollas), pp. 243–257. Berlin: Springer-Verlag.
CRENSHAW, M.A. and NEFF, J.M., 1969. Decalcification at the mantle-shell interface in molluscs. Amer. Zool., 9:881–885.
CRENSHAW, M.A. and RISTEDT, H., 1975. Histochemical and structural study of nautiloid septal nacre. Biomineralization, 8:1–8.
CRENSHAW, M.A. and RISTEDT, H., 1976. The histochemical localization of reactive groups in septal nacre from Nautilus pompilius. In The Mechanisms of Mineralization in the Invertebrates and Plants, (ed. N. Watabe and K.M. Wilbur), pp. 355–367. Univ. S. Carolina Press.
GARRELS, R.M. and THOMPSON, M.E., 1962. A chemical model for seawater at 25°C and one atmosphere total pressure. Amer. J. Sci., 260:57–66.
GREENFIELD, E.M., WILSON, D.C. and CRENSHAW, M.A., 1984. Ionotropic nucleation of calcium carbonate by molluscan matrix. Amer. Zool., 24:925–932.
LOWENSTAM, H.A., 1981. Minerals formed by organisms. Science, 211:1126–1131.
MISOGIANES, M.J. and CHASEEN, N.D., 1979. A chemical and spectral characterization of the extrapallial fluid of Mytilus edulis. Anal. Biochem., 100:324–334.
SIKES, C.S. and WHEELER, A.P., 1983. A systematic approach to some fundamental questions of carbonate calcification. In Biomineralization and Biological Metal Accumulation, (ed. P. Westbroek and E.W. deJong), pp. 285–289. Holland: Reidel Publishing.
SIKES, C.S. and WHEELER, A.P., 1986. The organic matrix from oyster shell as a regulator of calcification in vivo. Biol. Bull., Mar. Biol. Lab., Woods Hole, Mass., 170:494–505.
STUMM, W. and MORGAN, J.J., 1970. Aquatic Chemistry, 583 pp. New York: John Wiley.
WESTALL, J.C., ZACHARY, J.L. and MOREL, F.M.M., 1976. MINEQL, a computer program for the calculation of chemical equilibrium composition of aqueous systems. Mass. Inst. Tech., Depart. Civil Eng. Tech. Note, 18:1–91.
WHEELER, A.P., GEORGE, J.W. and EVANS, C.A., 1981. Control of calcium carbonate nucleation and crystal growth by soluble matrix of oyster shell. Science, 212:1397–1398.
WHEELER, A.P. and SIKES, C.S., 1984. Regulation of carbonate calcification by organic matrix. Amer. Zool., 24:933–944.
WILBUR, K.M., 1964. Shell formation and regeneration. In Physiology of Mollusca, (ed. K.M. Wilbur and C.M. Yonge), pp. 243–282, New York: Academic Press.
WORMS, D. and WEINER, S., 1986. Mollusk shell organic matrix: fourier transform infrared study of the acidic macromolecules. J. Exp. Zool., 237:11–20.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Springer Science+Business Media New York
About this chapter
Cite this chapter
Greenfield, E.M., Crenshaw, M.A. (1989). Mineral Induction by the Soluble Matrix from Molluscan Shells. In: Crick, R.E. (eds) Origin, Evolution, and Modern Aspects of Biomineralization in Plants and Animals. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-6114-6_23
Download citation
DOI: https://doi.org/10.1007/978-1-4757-6114-6_23
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-6116-0
Online ISBN: 978-1-4757-6114-6
eBook Packages: Springer Book Archive