Fluorescence Spectroscopy of Turbid Media

  • Rebecca Richards-Kortum
Part of the Lasers, Photonics, and Electro-Optics book series (LPEO)


The diagnosis of disease is becoming more and more a technologic task. The clinician’s goal is to assess the structural and functional changes in diseased tissue, infer the identity and stage of the disease, and predict the ultimate consequences to the organism as a whole, intervening with the proper treatment whenever possible.1 The diagnostic ordnance varies, both for the suspected disease and with the specialty of the clinician. Radiologists, for example, assess gross structural abnormalities utilizing variations in tissue or contrast agent absorption of X-rays. This structural information, although useful diagnostically, provides limited insight into the molecular etiology and pathogenesis of the disease, factors now appreciated to be important prognostically and in selecting appropriate therapy.1 Pathology provides the most widely used clinical method of elucidating chemical information from diseased tissues.1.2 Traditional techniques of histology probe the microscopic structural alterations of diseased tissue. Using histochemical stains, many of the corresponding chemical alterations can be mapped out on a microscopic scale. The chief disadvantage of histologic techniques is that they can only be applied in vitro, necessitating the removal of tissue.2 The requirement of biopsy limits the utility of this approach; it implies that only small areas of tissue, accessible to either biopsy forceps or needles, can be sampled.


Fluorescence Spectrum Fluorescence Spectroscopy Fluorescence Quantum Yield Flavin Adenine Dinucleotide Exit Angle 
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  1. 1.
    Robbins SL, Cotran RS, Kumar V. Pathologic Basis of Disease, 3rd ed., WB Saunders Co., Philadelphia, 1984.Google Scholar
  2. 2.
    Ham AW, Cormack DH. Histology, 8th ed., JB Lippincott Co., Philadelphia, 1979, pp. 3–32.Google Scholar
  3. 3.
    Lakowicz J. Principles of Fluorescence Spectroscopy, Plenum Press, New York, 1983.CrossRefGoogle Scholar
  4. 4.
    Slater PN. Remote Sensing, Optics, and Optical Systems, Addison Wesley, Reading, MA, 1980.Google Scholar
  5. 5.
    Richards-Kortum RR, Rava R, Fitzmaurice M, Tong L, Ratliff NB, Kramer JR, Feld MS. “A one-layer model of laser induced fluorescence for diagnosis of disease in human tissue: Applications to atherosclerosis,” IEEE Trans. Biomed. Eng. 36: 1222–1232, (1989).CrossRefGoogle Scholar
  6. 6.
    Laifer LI, O’Brien KMM, Stetz ML, Gindi GR, Garrand TJ, Deckelbaum LI. “Biochemical basis for the difference between normal and atherosclerotic arterial fluorescence,” Circulation 80: 1893–1901 (1989).CrossRefGoogle Scholar
  7. 7.
    Alfano RR, Pradhan A, Tang GC. “Optical spectroscopic diagnosis of cancer and normal breast tissues,” J. Opt. Soc. Am. 6: 1015–1023 (1989).ADSGoogle Scholar
  8. 8.
    Anderson PS, Montan S, Svanberg S. “Multispectral system for medical fluorescence imaging,” IEEE J. Quantum Electron. QE23: 1798 (1987).ADSCrossRefGoogle Scholar
  9. 9.
    Leon MB, Lu DY, Prevosti LG, Macy WW, Smith PD, Granovsky M, Bonner RF, Balaban RS. “Human arterial surface fluorescence: Atherosclerotic plaque identification and effects of laser atheroma ablation,” J. Am. College Cardiol. 12: 94–102 (1988).CrossRefGoogle Scholar
  10. 10.
    Schomaker KT, Frisoli JK, Compton CC, Flotte TJ, Richter JM, Nishioka NS, Deutsch TF. “Ultraviolet laser-induced fluorescence of colonic tissue: Basic biology and diagnostic potential,” Lasers Surg. Med. 12: 63–78 (1991).CrossRefGoogle Scholar
  11. 11.
    Cheong WF, Welch AJ. “A review of the optical properties of tissues,” IEEE J. Quantum Electron. 26: 2166–85 (1990).ADSCrossRefGoogle Scholar
  12. 12.
    Malinowski E. Factor Analysis in Chemistry, Wiley, New York, 1991.zbMATHGoogle Scholar
  13. 13.
    Tanke HJ, Oostveldt P van, Duijn P van. “A parameter for the distribution of fluorophores in cells derived from measurements of inner filter effect and reabsorption phenomenon,” Cytometry 2: 359–369 (1982).CrossRefGoogle Scholar
  14. 14.
    Ho CN, Christian GD, Davidson ER. “Application of the method of rank annihilation to quantitative analysis of multicomponent fluorescence data from the video fluorometer,” Anal. Chem. 50: 1108–1113 (1978).CrossRefGoogle Scholar
  15. 15.
    Ho CN, Christian GDS, Davidson ER. “Application of the method of rank annihilation to fluorescent multicomponent mixtures of polynuclear aromatic hydrocarbons,” Anal. Chem. 52: 1071–1079 (1980).CrossRefGoogle Scholar
  16. 16.
    Lorber, Avraham. “Quantifying chemical composition from two-dimensional data arrays,” Anal. Chim. Acta 164: 293–297 (1984).CrossRefGoogle Scholar
  17. 17.
    Campbell ID, Dwek RA. Biological Spectroscopy, Benjamin Cummings, Menlo Park, Ca., 1984, pp. 7–36.Google Scholar
  18. 18.
    Ishimaru I. Wave Propagation and Scattering in Random Media, Academic Press, New York, 1978.Google Scholar
  19. 19.
    Keijzer M, Richards-Kortum RR, Jacques SL, Feld M. “Fluorescence spectroscopy of turbid media: Autofluorescence of human aorta,” Appl. Opt. 28: 4286–4292 (1989).ADSCrossRefGoogle Scholar
  20. 20.
    Prahl S, “Light transport in tissue,” PhD Dissertation, The University of Texas at Austin, 1988.Google Scholar
  21. 21.
    Durkin A, Jaikumar S, Ramanujam N, Richards-Kortum R. “Relation between fluorescence spectra of dilute and turbid samples,” Appl. Opt. 33: 414–423 (1994).ADSCrossRefGoogle Scholar
  22. 22.
    Wu J, Feld MS, Rava RP. “An analytical model for extracting intrinsic fluorescence in a turbid media,” Appl. Opt. 32: 3585–3595 (1993).ADSCrossRefGoogle Scholar
  23. 23.
    Taylor DG, Demas JN. “Light intensity measurements I: Large area bolometers with µwatt sensitivities and absolute calibration of the Rhodamine B quantum counter,” Anal. Chem. 51: 7112–7117 (1979).Google Scholar
  24. 24.
    Prahl, S. “Light transport in tissue,” PhD Dissertation, The University of Texas at Austin, 1988.Google Scholar
  25. 25.
    Van Gemert MJC, Star WM. “Relations between the Kubelka–Munk and the transport equation models for anisotropic scattering,” Lasers Life Sci. 1: 287–298 (1987).Google Scholar
  26. 26.
    Ishimaru I. Wave Propagation and Scattering in Random Media, Vol. I, Academic Press, New York, 1978.Google Scholar
  27. 27.
    Durkin AJ, Jaikumar S, Richards-Kortum RR. “Optically dilute, absorbing and turbid phantoms for fluorescence spectroscopy of homogeneous and inhomogeneous samples,” Appl. Spectrosc. 47: 2114–2121 (1993).ADSCrossRefGoogle Scholar
  28. 28.
    Bohren CF, Huffman DR. Absorption and Scattering of Light by Small Particles, Wiley, New York, 1983.Google Scholar
  29. 29.
    Cothren RM, Richards-Kortum RR, Sivak MV, Fitzmaurice M, Rava RP, Boyce GA, Hayes GB, Doxtader M, Blackman R, Ivanc T, Feld MS, Petras RE. “Gastrointestinal tissue diagnosis by laser induced fluorescence spectroscopy at endoscopy,” Gastrointest. Endosc. 36: 105–111 (1990).CrossRefGoogle Scholar
  30. 30.
    Bigio I. “Optical biopsy for cancer detection,” Talk, Spectroscopic Approaches to Analysis of Biological Tissue, Albuquerque, NM, July, 1992.Google Scholar
  31. 31.
    Richards-Kortum RR, Mehta A, Hayes G, Cothren R, Kolubayev T, Kittrell C, Ratliff NB, Kramer JR, Feld MS. “Spectral diagnosis of atherosclerosis using an optical fiber laser catheter,” Am. Heart J. 118(2): 381 (1989).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Rebecca Richards-Kortum
    • 1
  1. 1.Department of Electrical and Computer EngineeringThe University of Texas at AustinAustinUSA

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