Temporal Control of Lux Gene Expression in the Symbiosis between Vibrio Fischeri and Its Squid Host

  • Karen L. Visick
  • Edward G. Ruby
Chapter

Abstract

A long-standing question in the study of luminous bacterial symbionts has been: What is the importance of light-emission to the bacteria? We have used the symbiosis between Vibrio fischeri and the Hawaiian sepiolid squid Euprymna scolopes as a model system (McFall-Ngai & Ruby, 1991; Ruby, 1996) with which to discover the answer to this question. Within hours of hatching, juvenile squids become inoculated by a few cells of V. fischeri present in the ambient seawater (Ruby & Asato, 1993). These bacteria rapidly colonize the epithelium-lined crypts of the nascent light-emitting organ, attaining a population size of about one million cells. Their light emission is used by the host in its nocturnal behavior (McFall-Ngai, 1997), and evidence is accumulating that suggests that the host regulates the bacterial luminescence on multiple levels (Boetttcher & Ruby, 1995; Boettcher et al., 1996).

Keywords

Light Organ Homoserine Lactone Ambient Seawater Lacl0 Repressor Aldehyde Substrate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Karen L. Visick
    • 1
  • Edward G. Ruby
    • 1
  1. 1.Pacific Biomedical Research CenterUniversity of HawaiiHonoluluUSA

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