Dereplication and Profiling of Marine Bacteria by Fatty Acid Analysis of Crude Extracts Using Fourier Transform Mass Spectrometry
Methods for grouping of bacteria include analysis of fatty acids by pyrolysis mass spectrometry of free fatty acids (Sanglier et al., 1992), their methyl esters (Basile et al., 1995), phospholipids and glycolipids by fast atom bombardment mass spectrometry (Heller et al., 1987), measurement of fatty acid content by methylation and gas chromatographic analysis of methyl esters (Bertone et al., 1996), GCMS (Dzierzewicz et al., 1996), and mass spectral fingerprints generated by matrix assisted laser desorption of intact bacterial cells (Claydon et al., 1995). Methods developed for grouping terrestrial bacteria can be transferred to marine species (Basile et al., 1995). There has been considerable recent interest in methods for phylogenetic grouping and identification of marine bacteria, where traditional biochemical tests are not effective in grouping isolates.
KeywordsMarine Bacterium Total Fatty Acid Content High Mass Accuracy FOURIER Transform Mass Spectrometry Fast Atom Bombardment Mass Spectrometry
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