Abstract
We have extensively characterized the structure and evolution of the adrenergic and muscarinic cholinergic receptors. This analysis has included studies with monoclonal antibodies, protein purification, target size analysis, gene cloning and sequencing and gene expression (1–7). Based upon the results of biochemical and immunological studies, we proposed that adrenergic and muscarinic cholinergic receptors are highly conserved proteins. This hypothesis has been confirmed by gene cloning experiments which have provided the primary structures of a number of adrenergic and muscarinic cholinergic receptors.
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Fraser, C.M., and Venter, J.C., Monoclonal antibodies to beta-adrenergic receptors: their use in purification and molecular characterization of beta-receptors, Proc. Natl. Acad. Sci. USA 77: 7034 (1980).
Venter, J.C., Muscarinic cholinergic receptor structure. Receptor size, membrane orientation and absence of major phylogenetic structural diversity, J Biol Chem 258: 4842 (1983)
Venter, J.C., Eddy, B., Hall, L.M., and Fraser, C.M., Monoclonal antibodies detect the conservation of muscarinic cholinergic receptor structure from Drosophila to human brain and detect possible structural homology with alphal-adrenergic receptors, Proc Natl Acad Sci USA 1: 1725 (1984).
Shreeve, S.M., Fraser, C.M., and Venter, J.C., Molecular comparison of alphal and alpha2-adrenergic receptors suggest that these proteins are structurally related “isoreceptors”, Proc Natl Acad Sci USA 2: 4842 (1985).
Chung, F.Z., Lentes, K.U., Gocayne, J., FitzGerald, M., Robinson, D.A., Kerlavage, A.R., Fraser, C.M., and Venter, J.C., Cloning and sequence analysis of the human brain beta-adrenergic receptor, FEBS Lett 211: 200 (1987).
Gocayne, J., Robinson, D.A., FitzGerald, M.G., Chung, F.Z., Kerlavage, A.R., Lentes, K.U., Lai, J., Wang, C.D., Fraser, C.M., and Venter, J.C., Primary structure of rat cardiac beta-adrenergic and muscarinic cholinergic receptors obtained by automated DNA sequence analysis: further evidence for a multigene family, Proc. Natl. Acad. Sci. USA, in press.
Fraser, C.M., Chung, F.Z., and Venter, J.C., High density expression of human beta2-adrenergic receptors in a mouse cell line previously lacking beta-receptors, J. Biol. Chem. 262: 14843 (1987).
Dixon, R.A.F., Kobilka, B.K., Strader, D.J., Benovic, J.L., Dohlman, H.G., Frielle, T., Bolanowski, M.A., Bennett, C.D., Rands, E., Diehl, R.E., Mumford, R.A., Slater, E.E., Sigal, I.S., Caron, M.G., Lefkowitz, R.J., and Strader, C.D., Cloning of the gene and cDNA for mammalian beta-adrenergic receptor and homology with rhodopsin, Nature 321: 75 (1986).
Yarden, Y., Rodriguez, H., Wong, S.K.F., Brandt, D.R., May, D.C., Burnier, J., Harkins, R.N., Chen, E.Y., Ramachandran, J., Ullrich, A., Ross, E.M., The avian beta-adrenergic receptor: primary structure and membrane topology, Proc Natl Acad Sci USA 83: 6795 (1986).
Kubo, T., Maeda, A., Sugimoto, K., Akiba, I., Mikami, A., Takahashi, H., Haga, T., Haga, K., Ichiyama, A., Kangawa, K., Matsuo, H., Hirose, T., and Numa, S., Primary structure of porcine cardiac muscarinic acetylcholine receptor deduced from the cDNA sequence, FEBS Lett 209: 367 (1986).
Peralta, E.G., Winslow, J.W., Peterson, G.L., Smith, D.H., Ashkenazi, A., Ramachandran, J., Schimerlik, M.I., and Capon, D.J., Cardiac muscarinic receptor primary structure and gene organization: atrial and cerebral receptor subtypes are distinct gene products, Science 236: 600 (1987).
Kubo, T., Fukuda, K., Mikami, A., Maeda, A., Takahashi, H., Mishina, M., Haga, T., Haga, K., Ichiyama, A., Kangawa, K., Kojima, M., Matsuo, H., Hirose, T., and Numa, S., Cloning, sequencing and expression of complementary cDNA encoding the muscarinic acetylcholine receptor, Nature 323: 411 (1986).
Kerlavage, A.R., Fraser, C.M., Chung, F.Z., and Venter, J.C., Molecular structure and evolution of adrenergic and cholinergic receptors, Proteins 1: 287 (1986).
Applebury, M.L. and Hargrave, P.A., Molecular biology of the visual pigments, Vision Res. 26: 1881 (1986).
Dixon, R.A.F., Sigal, I.S., Rands, E., Register, R.B., Candelore, M.R., Blake, A.D. and Strader, C.D., Ligand binding to the beta-adrenergic receptor involves its rhodopsin-like core. Nature 326: 73 (1987).
Chung, F.-Z., Wang, C.-D., Potter, P.C., Venter, J.C. and Fraser, C.M., Site-directed mutagenesis and continuous expression of human beta-adrenergic receptors. Identification of a conserved aspartate residue involved in agonist binding and receptor activation. J Biol Chem 263: in press, (1988).
Lad, P.M., Welton, A.F. and Rodbell, M., Evidence for distinct guanine nucleotide sites in the regulation of the glucagon receptor and of adenylate cyclase activity. J Biol Chem 252: 5942 (1977).
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Venter, J.C. et al. (1988). Cloning and Expression of Adrenergic and Muscarinic Cholinergic Receptor Genes. In: Kito, S., Segawa, T., Kuriyama, K., Tohyama, M., Olsen, R.W. (eds) Neuroreceptors and Signal Transduction. Advances in Experimental Medicine and Biology, vol 236. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5971-6_14
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DOI: https://doi.org/10.1007/978-1-4757-5971-6_14
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