Application of Fast Reaction Techniques to Kinetic Measurements of Receptor Function on Cell Surfaces

  • Norio Matsubara
  • George P. Hess
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 236)


Receptor proteins in nerve and muscle cells have attracted much attention recently because of their central role in the transmission of signals between nerve and muscle cells. Kinetic investigations of their function have been hampered because endogenous compounds on binding to the protein not only cause it to form an inorganic ion conducting transmembrane channel, but also induce rapid first-order state transitions from the active protein form to an inactive form with altered abilities to bind ligands. Two new approaches allow kinetic measurements of receptor function to be made on cell surfaces with a time resolution of μs to ms.


Acetylcholine Receptor Receptor Function Current Amplitude Receptor Molecule Receptor Desensitization 
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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Norio Matsubara
    • 1
  • George P. Hess
    • 1
  1. 1.Section of Biochemistry, Molecular and Cell Biology, Division of Biological SciencesCornell UniversityIthacaUSA

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