Skip to main content
SpringerLink
Log in

Structure and Localization of Glycoproteins and Proteoglycans

  • Chapter
Neurobiology of Glycoconjugates

Abstract

Glycosaminoglycans are high-molecular-weight linear carbohydrate polymers that are generally composed of disaccharide repeating units of a uronic acid (D-glucuronic acid or L-iduronic acid) and a hexosamine (G1cNAc or GaINAc). Chondroitin sulfate and heparan sulfate occur as proteoglycans in which the polysaccharide chains are covalently linked at their reducing ends to the hydroxyl groups of serine residues in a protein moiety.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Abbreviations

Con A:

concanavalin A

NGF:

nerve growth factor

NILE:

NGFinducible large external glycoprotein

AcNeu:

N-acetylneuraminic acid

Fuc:

l-fucose

Gal:

d-galactose

Ga1NAc:

n-acetyl-d-galac-tosamine

GlcNAcol:

n-acetyl-d-glucosaminitol

GaINAcol:

n-acetyl-d-galactosaminitol

GcNeu:

n-glycolylneuraminic acid

Glc:

d-glucose

GlcNAc:

n-acetyl-d-glucosamine

Man:

d-mannose

Manol:

mannitol

DBH:

dopamine β-hydroxylase

References

  • Abbs, M. T., and Phillips, J. H., 1980, Organization of the proteins of the chromaffin granule membrane, Biochim. Biophys. Acta 595: 200–221.

    PubMed  CAS  Google Scholar 

  • Abeijon, C., and Hirschberg, C. B., 1988, Intrinsic membrane glycoproteins with cytosol-oriented sugars in the endoplasmic reticulum, Proc. Natl. Acad. Sci. USA 85: 1010–1014.

    PubMed  CAS  Google Scholar 

  • Amblard, F., He, J.-T., Barbet, J., Goridis, C., and Prochiantz, A., 1988, A 140-kilodalton protein is released from cultured astrocytes by phosphatidylinositol phospholipase C, J. Neurochem. 50: 486–489.

    PubMed  CAS  Google Scholar 

  • Anderson, M. J., and Fambrough, D. M., 1983, Aggregates of acetylcholine receptors are associated with plaques of a basal lamina heparan sulfate proteoglycan on the surface of skeletal muscle fibers, J. Cell Biol. 97: 1396–1411.

    CAS  Google Scholar 

  • Anderson, M. J., Klier, F. G., and Tanguay, K. E., 1984, Acetylcholine receptor aggregation parallels the deposition of a basal lamina proteoglycan during development of the neuromuscular junction, J. Cell Biol. 99: 1769–1784.

    PubMed  CAS  Google Scholar 

  • Aquino, D., Wong, R., Margolis, R. U., and Margolis, R. K., 1980, Sialic acid residues inhibit proteolytic degradation of dopamine 3-hydroxylase, Febs Lett. 112: 195–198.

    PubMed  CAS  Google Scholar 

  • Aquino, D. A., Margolis, R. U., and Margolis, R. K., 1984a, Immunocytochemical localization of a chondroitin sulfate proteoglycan in nervous tissue. I. Adult brain, retina and peripheral nerve, J. Cell Biol. 99: 1117–1129.

    PubMed  CAS  Google Scholar 

  • Aquino, D. A., Margolis, R. U., and Margolis, R. K., 1984b, Immunocytochemical localization of a chondroitin sulfate proteoglycan in nervous tissue. II. Studies in developing brain, J. Cell Biol. 99: 1130–1139.

    PubMed  CAS  Google Scholar 

  • Bach, G., and Berman, E. R., 197la, Amino sugar-containing compounds of the retina. I. Isolation and identification, Biochim. Biophys. Acta 252: 453–461.

    Google Scholar 

  • Bach, G., and Berman, E. R., 1971b, Amino sugar-containing compounds of the retina. II. Structural studies, Biochim. Biophys. Acta 252: 461–471.

    Google Scholar 

  • Banerjee, S., and Margolis, R. U., 1982, Glycoproteins and proteoglycans of the chromaffin granule matrix, J. Neurochem. 39: 1700–1703.

    PubMed  CAS  Google Scholar 

  • Beasley, L., and Stallcup, W. B., 1987, The nerve growth factor-inducible large external (Nile) glycoprotein and neural cell adhesion molecule (N-Cam) have distinct patterns of expression in the developing rat central nervous system, J. Neurosci. 7: 708–715.

    PubMed  CAS  Google Scholar 

  • Benedum, U. M., Baeuerle, P. A., Konecki, D. S., Frank, R., Powell, J., Mallet, J., and Huttner, W. B., 1986, The primary structure of bovine chromogranin A: A representative of a class of acidic secretory proteins common to a variety of peptidergic cells, Embo J. 5: 1495–1502.

    PubMed  CAS  Google Scholar 

  • Benedum, U. M., Lamouroux, A., Konecki, D. S., Rosa, P., Hille, A., Baeuerle, P. A., Frank, R., Lottspeich, F., Mallet, J., and Huttner, W. B., 1987, The primary structure of human secretogranin I (chromogranin B): Comparison with chromogranin A reveals homologous terminal domains and a large intervening variable region, Embo J. 6: 1203–1211.

    PubMed  CAS  Google Scholar 

  • Bock, E., Richter-Landsberg, C., Faissner, A., and Schachner, M., 1985, Demonstration of immunochemical identity between the nerve growth factor-inducible large external (Nile) glycoprotein and the cell adhesion molecule Ll, Embo J. 4: 2765–2768.

    PubMed  CAS  Google Scholar 

  • Bolin, L. M., and Rouse, R. V., 1986, Localization of Thy-1 expression during postnatal development of the mouse cerebellar cortex, J. Neurocytol. 15: 29–36.

    PubMed  CAS  Google Scholar 

  • Brandan, E., Maldonado, M., Garrido, J., and Inestrosa, N. C., 1985, Anchorage of collagen-tailed acetylcholinesterase to the extracellular matrix is mediated by heparan sulfate proteoglycans, J. Cell Biol. 101: 985–992.

    Google Scholar 

  • Campbell, D. G. Gagnon, J., Reid, K. B. M., and Williams, A. F., 1981, Rat brain Thy-1 glycoprotein. The amino acid sequence, disulphide bonds and an unusual hydrophobic region, Biochem. J. 195: 15–30.

    PubMed  CAS  Google Scholar 

  • Carey, D. J., and Todd, M. S., 1986, A cytoskeleton-associated plasma membrane heparan sulfate proteoglycan in Schwann cells, J. Biol. Chem. 261: 7518–7525.

    PubMed  CAS  Google Scholar 

  • Chen, H.-C., Shimohigashi, Y., Dufau, M. L., and Catt, K. J., 1982, Characterization and biological properties of chemically deglycosylated human chorionic gonadotropin, J. Biol. Chem. 257: 14446–14452.

    PubMed  CAS  Google Scholar 

  • Choi, H. U., and Meyer, K., 1975, The structure of a sulfated glycoprotein of chick allantoic fluid: Methylation and periodate oxidation, Carbohyd. Res. 40: 77–88.

    CAS  Google Scholar 

  • Cole, G. J., Schubert, D., and Glaser, L., 1985, Cell–substratum adhesion in chick neural retina depends upon protein–heparan sulfate interactions, J. Cell Biol. 100: 1192–1199.

    PubMed  CAS  Google Scholar 

  • Cole, G. J., Loewy, A., and Glaser, L., 1986, Neuronal cell-cell adhesion depends on interactions of N-Cam with heparin-like molecules, Nature 320: 445–447.

    PubMed  CAS  Google Scholar 

  • Davis, L. I., and Blobel, G., 1987, Nuclear pore complex contains a family of glycoproteins that includes p62: Glycosylation through a previously unidentified cellular pathway, Proc. Natl. Acad. Sci. USA 84: 7552–7556.

    PubMed  CAS  Google Scholar 

  • Edgar, D., Timpl, R., and Thoenen, H., 1984, The heparin-binding domain of laminin is responsible for its effects on neurite outgrowth and neuronal survival, Embo J. 3: 1463–1468.

    PubMed  CAS  Google Scholar 

  • Edge, A. S. B., and Spiro, R. G., 1984, Presence of sulfate in N-glycosidically linked carbohydrate units of calf thyroid plasma membrane glycoproteins, J. Biol. Chem. 259: 4710–4713.

    PubMed  CAS  Google Scholar 

  • Ferwerda, W., Blok, C. M., and Heijlman, J., 1981, Turnover of free sialic acid, Cmp-sialic acid, and bound sialic acid in rat brain, J. Neurochem. 36: 1492–1499.

    PubMed  CAS  Google Scholar 

  • Finne, J., 1985, Polysialic acid—a glycoprotein carbohydrate involved in neural adhesion and bacterial meningitis, Trends Biochem. Sci. 10: 129–132.

    CAS  Google Scholar 

  • Finne, J., and Krusius, T., 1976, O-glycosidic carbohydrate units from glycoproteins of different tissues: Demonstration of a brain-specific disaccharide, a-galactosyl(1–3)N-acetylgalactosamine, Febs Lett. 66: 94–97.

    PubMed  CAS  Google Scholar 

  • Finne, J. ,and Krusius, T., 1982, Preparation and fractionation of glycopeptides, Methods Enzymol. 83: 269–277.

    PubMed  CAS  Google Scholar 

  • Finne, J., and Mäkelä, P. H., 1985, Cleavage of the polysialosyl units of brain glycoproteins by a bacteriophage endosialidase, J. Biol. Chem. 260: 1265–1270.

    PubMed  CAS  Google Scholar 

  • Finne, J., Krusius, T., Rauvala, H., and Hemminki, K., 1977, The disialosyl group of glycoproteins: Occurrence in different tissues and cellular membranes, Eur. J. Biochem. 77: 319–323.

    PubMed  CAS  Google Scholar 

  • Finne, J., Krusius, T., Margolis, R. K., and Margolis, R. U., 1979, Novel mannitol-containing oligosaccharides obtained by mild alkaline borohydride treatment of a chondroitin sulfate proteoglycan from brain, J. Biol. Chem. 254: 10295–10300.

    PubMed  CAS  Google Scholar 

  • Fischer-Colbrie, R., Schachinger, M., Zangerle, R., and Winkler, H., 1982, Dopamine ß-hydroxylase and other glycoproteins from the soluble content and the membranes of adrenal chromaffin granules: Isolation and carbohydrate analysis, J. Neurochem. 38: 725–732.

    PubMed  CAS  Google Scholar 

  • Fischer-Colbrie, R., Zangerle, R., Frischenschlager, I., Weber, A., and Winkler, H., 1984, Isolation and immunological characterization of a glycoprotein from adrenal chromaffin granules, J. Neurochem. 42: 1008–1016.

    PubMed  CAS  Google Scholar 

  • Flanagan, B. F., Teplow, D. B., Dreyer, W. J., and Fabre, J. W., 1986, Unusual phylogenetic conservation of the N–terminal amino acid sequence of the central nervous system–specific membrane glycoprotein F3–87–8 (Cnsgp130), J. Neurochem. 46: 542 – 544.

    PubMed  CAS  Google Scholar 

  • Fransson, L.-A., Hampson, I., Kumar, S., and Gallagher, J., 1985, Chemical heterogeneity of heparan sulfate from a human neuroblastoma cell line, Acta Chem. Scand. B 39: 305–313.

    PubMed  CAS  Google Scholar 

  • Friedlander, D., Grumet, M., and Edelman, G., 1986, Nerve growth factor enhances expression of neuronglia cell adhesion molecule in PC12 cells, J. Cell Biol. 102: 413–419.

    PubMed  CAS  Google Scholar 

  • Gallagher, J. T., Lyon, M., and Steward, W. P., 1986, Structure and function of heparan sulfate proteoglycans, Biochem. J. 236: 313–325.

    PubMed  CAS  Google Scholar 

  • Gallo, V., Bertolotto, A., and Levi, G., 1987, The proteoglycan chondroitin sulfate is present in a sub-population of cultured astrocytes and in their precursors, Dev. Biol. 123: 282–285.

    PubMed  CAS  Google Scholar 

  • Gammon, C. M., Goodrum, J. F., Toews, A. D., Okabe, A., and Morell, P., 1985, Axonal transport of glycoconjugates in the rat visual system, J. Neurochem. 44: 376–387.

    PubMed  CAS  Google Scholar 

  • Gandy, S., Czernick, A. J., and Greengard, P., 1988, Phosphorylation of Alzheimer disease amyloid precursor peptide by protein kinase C and Cat2+/calmodulin-dependent protein kinase II, Proc. Natl. Acad. Sci. Usa 85: 6218–6221.

    PubMed  CAS  Google Scholar 

  • Gavine, F. S., Pryde, J. G., Deane, D. L., and Apps, D. K., 1984, Glycoproteins of the chromaffin granule membrane: Separation by two-dimensional electrophoresis and identification by lectin binding, J. Neurochem. 43: 1243–1252.

    PubMed  CAS  Google Scholar 

  • Geissler, D., Martinek, A., Margolis, R. U., Margolis, R. K., Skrivanek, J. A., Ledeen, R. W., König, P., and Winkler, H., 1977, Composition and biogenesis of complex carbohydrates of adrenal chromaffin granules, Neuroscience 2: 685–693.

    PubMed  CAS  Google Scholar 

  • Giannattasio, G., Zanini, A., and Meldolesi, J., 1979, Complex carbohydrates of secretory organelles, in: Complex Carbohydrates of Nervous Tissue (R. U. Margolis and R. K. Margolis, eds.), pp. 327–345, Plenum Press, New York.

    Google Scholar 

  • Giannattasio, G., Zanini, A., Rosa, P., Meldolesi, J., Margolis, R. K., and Margolis, R. U., 1980, Molecular organization of prolactin granules-III. Intracellular transport of glycosaminoglycans and glycoproteins of the bovine prolactin granule matrix, J. Cell Biol. 86: 273–279.

    PubMed  CAS  Google Scholar 

  • Ginsburg, V. (ed.), 1982, Complex Carbohydrates, Part D, (Meth. Enzymol., Vol. 83 ), Academic Press, New York.

    Google Scholar 

  • Ginsburg, V. (ed.), 1987, Complex Carbohydrates, Part E, (Meth. Enzymol., Vol. 138 ), Academic Press, New York.

    Google Scholar 

  • Gowda, D. C., Margolis, R. U., and Margolis, R. K., 1989a, Presence of the Hnk-1 epitope on poly(Nacetyllactosaminyl) oligosaccharides and identification of multiple core proteins in the chondroitin sulfate proteoglycans of brain, Biochemistry,in press.

    Google Scholar 

  • Gowda, D. C., Margolis, R. K., Frangione, B., Ghiso, J., Larrondo-Lillo, M., and Margolis, R. U., 1989b, Relation of the amyloid 13 protein precursor to heparan sulfate proteoglycans, Science,in press.

    Google Scholar 

  • Gowda, D. C., Goossen, B., Margolis, R. K., and Margolis, R. U., 1989c, Chondroitin sulfate and heparin sulfate proteoglycans of PC12 pheochromocytoma cells, J. Biol. Chem.,in press.

    Google Scholar 

  • Gralnick, H. R., Williams, S. B., and Rick, M. E., 1983, Role of carbohydrate in multimeric structure of factor Viii/von Willebrand factor protein, Proc. Natl. Acad. Sci. USA 80: 2771–2774.

    PubMed  CAS  Google Scholar 

  • Green, E. D., and Baenziger, J. U., 1988, Asparagine-linked oligosaccharides on lutropin, follitropin, and thyrotropin. I. Structural elucidation of the sulfated and sialylated oligosaccharides on bovine, ovine, and human pituitary glycoprotein hormones, J. Biol. Chem. 263: 25–35.

    PubMed  CAS  Google Scholar 

  • Greene, L. A., and Shelanski, M. L., 1989, The nerve growth factor-inducible large external (Nile) glycoprotein: Biochemistry and regulation of synthesis, in: Morphoregulatory Molecules ( G. M. Edelman, B. A. Cunningham, and J.-P. Thiery, eds.), Wiley, New York.

    Google Scholar 

  • Greene, L. A., and Tischler, A. S., 1982, PC12 pheochromocytoma cultures in neurobiological research, in: Advances in Cellular Neurobiology (S. Fedoroff and L. Hertz, eds.), Vol. 3, pp. 373–414, Academic Press, New York.

    Google Scholar 

  • Hageman, G. S., and Johnson, L. V., 1987, Chondroitin 6-sulfate glycosaminoglycan is a major constituent of primate cone photoreceptor matrix sheaths, Curr. Eye Res. 6: 639–646.

    PubMed  CAS  Google Scholar 

  • Hamos, J., Desai, P. R., and Villafranca, J. J., 1987, Characterization and kinetic studies of deglycosylated dopamine 13-hydroxylase, Faseb J. 1: 143–148.

    PubMed  CAS  Google Scholar 

  • Hampson, I. N., Kumar, S., and Gallagher, J. T., 1983, Differences in the distribution of 0-sulphate groups of cell-surface and secreted heparan sulphate produced by human neuroblastoma cells in culture, Biochim. Biophys. Acta 763: 183–190.

    PubMed  CAS  Google Scholar 

  • Hampson, I. N., Kumar, S., and Gallagher, J. T., 1984, Heterogeneity of cell-associated and secretory heparan sulphate proteoglycans produced by cultured human neuroblastoma cells, Biochim. Biophys. Acta 801: 306–313.

    PubMed  CAS  Google Scholar 

  • Hanover, J. A., Cohen, C. K., Willingham, M. C., and Park, M. K., 1987, 0-Linked N-acetylglucosamine is attached to proteins of the nuclear pore. Evidence for cytoplasmic and nucleoplasmic glycoproteins, J. Biol. Chem. 262: 9887–9894.

    Google Scholar 

  • Hascall, V. C., and Hascall, G. K., 1982, Proteoglycans, in: Cell Biology of Extracellular Matrix (E. D. Hay, ed.), pp. 39–63, Plenum Press, New York.

    Google Scholar 

  • Hassell, J. R., Kimura, J. H., and Hascall, V. C., 1986, Proteoglycan core protein families, Annu. Rev. Biochem. 55: 539–567.

    PubMed  CAS  Google Scholar 

  • Hewitt, A. T., 1986, Extracellular matrix molecules: Their importance in the structure and function of the retina, in: The Retina: A Model for Cell Biology Studies (R. Adler and D. Farber, eds.), Part II, pp. 169–214, Academic Press, New York.

    Google Scholar 

  • Hoffman, S., and Edelman, G. M., 1987, A proteoglycan with Hnk-1 antigenic determinants is a neuron-associated ligand for cytotactin, Proc. Natl. Acad. Sci. USA 84: 2523–2527.

    PubMed  CAS  Google Scholar 

  • Hoffman, S., Crossin, K. L., and Edelman, G. M., 1988, Molecular forms, binding functions, and developmental expression patterns of cytotactin and cytotactin-binding proteoglycan, an interactive pair of extracellular matrix molecules, J. Cell Biol. 106: 519–532.

    PubMed  CAS  Google Scholar 

  • Holt, G. D., Snow, C. M., Senior, A., Haltiwanger, R. S., Gerace, L., and Hart, G. W., 1987a, Nuclear pore complex glycoproteins contain cytoplasmically disposed 0-linked N-acetylglucosamine, J. Cell Biol. 104: 1157–1164.

    CAS  Google Scholar 

  • Holt, G. D., Haltiwanger, R. S., Tones, C.-R., and Hart, G. W., 1987b, Erythrocytes contain cytoplasmic glycoproteins, J. Biol. Chem. 262: 14847–14850.

    CAS  Google Scholar 

  • Hooghe-Peters, E. L., and Hooghe, R. J., 1982, The Thy-1 giycoprotein on nerve cells in culture, J. Neuroimmunol. 2: 191–200.

    PubMed  CAS  Google Scholar 

  • Höök, M., Kjellén, L., Johansson, S., and Robinson, J., 1984, Cell surface glycosaminoglycans, Annu. Rev. Biochem. 53: 847–869.

    PubMed  Google Scholar 

  • Huber, E., König, P., Schuler, G., Aberer, W., Plattner, H., and Winkler, H., 1979, Characterization and topography of the glycoproteins of adrenal chromaffin granules, J. Neurochem. 32: 35–47.

    PubMed  CAS  Google Scholar 

  • Iacangelo, A., Affolter, H.-U., Eiden, L. E., Herbert, E., and Grimes, M., 1986, Bovine chromogranin A sequence and distribution of its messenger Rna in endocrine tissues, Nature 323: 82–86.

    PubMed  CAS  Google Scholar 

  • Inestrosa, N. C., Matthew, W. D., Reiness, C. G., Hall, Z. W., and Reichardt, L. F., 1985, Atypical distribution of asymmetric acetylcholinesterase in mutant PC12 pheochromocytoma cells lacking a cell surface heparan sulfate proteoglycan, J. Neurochem. 45: 86–94.

    PubMed  CAS  Google Scholar 

  • James, W. M., and Agnew, W. S., 1987, Multiple oligosaccharide chains in the voltage-sensitive Na channel from Electrophorus electricus: Evidence for α-2, 8 linked polysialic acid, Biochem. Biophys. Res. Commun. 148: 817–826.

    PubMed  CAS  Google Scholar 

  • Johnson, L. V., and Hageman, G. S., 1987, Enzymatic characterization of peanut agglutinin-binding components in the retinal interphotoreceptor matrix, Exp. Eye Res. 44: 553–566.

    PubMed  CAS  Google Scholar 

  • Kalyan, N. K., and Bahl, O. P., 1983, Role of carbohydrate in human chorionic gonadotropin, J. Biol. Chem. 258: 67–74.

    PubMed  CAS  Google Scholar 

  • Kennedy, J. F., 1979, Proteoglycans—Biological and Chemical Aspects in Human Life, Elsevier, Amsterdam.

    Google Scholar 

  • Kiang, W.-L., Crockett, C. P., Margolis, R. K., and Margolis, R. U., 1978, Glycosaminoglycans and glycoproteins associated with microsomal subfractions of brain and liver, Biochemistry 17: 3841–3848.

    PubMed  CAS  Google Scholar 

  • Kiang, W.-L., Margolis, R. U., and Margolis, R. K., 1981, Fractionation and properties of a chondroitin sulfate proteoglycan and the soluble glycoproteins of brain, J. Biol. Chem. 256: 10529–10537.

    PubMed  CAS  Google Scholar 

  • Kiang, W.-L., Krusius, T., Finne, J., Margolis, R. U., and Margolis, R. K., 1982, Glycoproteins and proteoglycans of the chromaffin granule matrix, J. Biol. Chem. 257: 1651–1659.

    PubMed  CAS  Google Scholar 

  • Klinger, M. M., Margolis, R. U., and Margolis, R. K., 1985, Isolation and characterization of the heparan sulfate proteoglycans of brain. Use of affinity chromatography on lipoprotein lipase-agarose, J. Biol. Chem. 260: 4082–4090.

    PubMed  CAS  Google Scholar 

  • Korpeinen, T., Mononen, I., Krusius, T., and Järnefelt, J., 1982, Glycosylation of proteins in developing human brain, J. Neurochem. 39: 1737–1739.

    PubMed  CAS  Google Scholar 

  • Krusius, T., and Finne, J., 1977, Structural features of tissue glycoproteins. Fractionation and methylation analysis of glycopeptides derived from rat brain, kidney and liver, Eur. J. Biochem. 78: 369–379.

    PubMed  CAS  Google Scholar 

  • Krusius, T., and Finne, J., 1978, Characterization of a novel sugar sequence from rat brain glycoproteins containing fucose and sialic acid, Eur. J. Biochem. 84: 395–403.

    PubMed  CAS  Google Scholar 

  • Krusius, T., Finne, J., Margolis, R. U., and Margolis, R. K., 1978, Structural features of microsomal, synaptosomal, mitochondrial, and soluble glycoproteins of brain, Biochemistry 17: 3849–3854.

    PubMed  CAS  Google Scholar 

  • Krusius, T., Finne, J., Margolis, R. K., and Margolis, R. U., 1986, Identification of an O-glycosidic marnose-linked sialylated tetrasaccharide and keratan sulfate oligosaccharides in the chondroitin sulfate proteoglycan of brain, J. Biol. Chem. 261: 8237–8242.

    PubMed  CAS  Google Scholar 

  • Krusius, T., Reinhold, V. N., Margolis, R. K., and Margolis, R. U., 1987, Structural studies on sialylated and sulfated O-glycosidic marnose-linked oligosaccharides in the chondroitin sulfate proteoglycan of brain, Biochem. J. 245: 229–234.

    PubMed  CAS  Google Scholar 

  • Lakin, K. H., and Fabre, J. W., 1981, Identification with a monoclonal antibody of a phylogenetically conserved brain-specific determinant on a 130,000 molecular weight glycoprotein of human brain, J. Neurochem. 37: 1170–1178.

    PubMed  CAS  Google Scholar 

  • Lakin, K. H., Allen, A. K., and Fabre, J. W., 1983, Purification and preliminary biochemical characterization of the human and rat forms of the central nervous system–specific molecule, F3–87–8, J. Neurochem. 41: 385 – 394.

    PubMed  CAS  Google Scholar 

  • Lamouroux, A., Vigny, A., Biguet, N. F., Damon, M. C., Franck, R., Henry, J.-P., and Mallet, J., 1987, The primary structure of human dopamine-ß-hydroxylase: Insights into the relationship between the soluble and the membrane-bound forms of the enzyme, Embo J. 6: 3931–3937.

    PubMed  CAS  Google Scholar 

  • Lander, A. D., Fujii, D. K., and Reichardt, L. F., 1985, Laminin is associated with the “neurite outgrowth- promoting factors” found in conditioned medium, Proc. Natl. Acad. Sci. USA 82: 2183–2187.

    PubMed  CAS  Google Scholar 

  • Laslop, A., Fischer-Colbrie, R., Hook, V., Obendorf, D., and Winkler, H., 1986, Identification of two glycoproteins of chromaffin granules as the carboxypeptidase H, Neurosci. Lett. 72: 300–304.

    PubMed  CAS  Google Scholar 

  • Lechner, J., Wieland, F., and Sumper, M., 1985, Biosynthesis of sulfated saccharides N-glycosidically linked to the protein via glucose, J. Biol. Chem. 260: 860–866.

    PubMed  CAS  Google Scholar 

  • Levine, J. M., and Card, J. P., 1987, Light and electron microscopic localization of a cell surface antigen (NG2) in the rat cerebellum: Association with smooth protoplasmic astrocytes, J. Neurosci. 7: 2711 2720.

    Google Scholar 

  • Liau, Y. H., and Horowitz, M. I., 1982, Incorporation in vitro of [3H]glucosamine or [3H]glucose and [35S]SO4 2— into rat gastric mucosa, J. Biol. Chem. 257: 4709–4718.

    PubMed  CAS  Google Scholar 

  • Livingston, B. D., Jacobs, J. L., Glick, M. C., and Troy, F. A., 1988, Extended polysialic acid chains (n>55) in glycoproteins from human neuroblastoma cells, J. Biol. Chem. 263: 9443–9448.

    PubMed  CAS  Google Scholar 

  • Low, M. G., and Saltiel, A. R., 1988, Structural and functional roles of glycosyl-phosphatidylinositol in membranes, Science 239: 268–275.

    PubMed  CAS  Google Scholar 

  • Manjunath, P., and Sairam, M. R., 1982, Biochemical, biological, and immunological properties of chemically deglycosylated human choriogonadotropin, J. Biol. Chem. 257: 7109–7115.

    PubMed  CAS  Google Scholar 

  • Maresh, G. A., Chemoff, E. A. G., and Culp, L. A., 1984, Heparan sulfate proteoglycans of human neuroblastoma cells: Affinity fractionation on columns of platelet factor-4, Arch. Biochem. Biophys. 233: 428–437.

    PubMed  CAS  Google Scholar 

  • Margolis, R. U., 1967, Acid mucopolysaccharides and proteins of bovine whole brain, white matter and myelin, Biochim. Biophys. Acta 141: 91–102.

    PubMed  CAS  Google Scholar 

  • Margolis, R. K., and Margolis, R. U., 1970, Sulfated glycopeptides from rat brain glycoproteins, Biochemistry 9: 4389–4396.

    PubMed  CAS  Google Scholar 

  • Margolis, R. U., and Margolis, R. K., 1972, Sulfate turnover in mucopolysaccharides and glycoproteins of brain, Biochim. Biophys. Acta 264: 426–431.

    PubMed  CAS  Google Scholar 

  • Margolis, R. K., and Margolis, R. U., 1973, The turnover of hexosamine and sialic acid in glycoproteins and mucopolysaccharides of brain, Biochim. Biophys. Acta 304: 413–420.

    PubMed  CAS  Google Scholar 

  • Margolis, R. U., and Margolis, R. K., 1974, Distribution and metabolism of mucopolysaccharides and glycoproteins in neuronal perikaria, astrocytes, and oligodendroglia, Biochemistry 13: 2849–2852.

    PubMed  CAS  Google Scholar 

  • Margolis, R. K., and Margolis, R. U., 1979, Structure and distribution of glycoproteins and glycosaminoglycans, in: Complex Carbohydrates of Nervous Tissue ( R. U. Margolis and R. K. Margolis, eds.), pp. 45–73, Plenum Press, New York.

    Google Scholar 

  • Margolis, R. K., and Margolis, R. U., 1983, Distribution and characteristics of polysialosyl oligosaccharides in nervous tissue glycoproteins, Biochem. Biophys. Res. Commun. 116: 889–894.

    PubMed  CAS  Google Scholar 

  • Margolis, R. K., Margolis, R. U., Preti, C., and Lai, D., 1975a, Distribution and metabolism of glycoproteins and glycosaminoglycans in subcellular fractions of brain, Biochemistry 14: 4797–4804.

    PubMed  CAS  Google Scholar 

  • Margolis, R. K., Preti, C., Chang, L., and Margolis, R. U., 1975b, Metabolism of the protein moiety of brain glycoproteins, J. Neurochem. 25: 707–709.

    PubMed  CAS  Google Scholar 

  • Margolis, R. K., Crockett, C. P., Kiang, W.-L., and Margolis, R. U., 1976a, Glycosaminoglycans and glycoproteins associated with rat brain nuclei, Biochim. Biophys. Acta 451: 465–469.

    PubMed  CAS  Google Scholar 

  • Margolis, R. K., Preti, C., Lai, D., and Margolis, R. U., 1976b, Developmental changes in brain glycoproteins, Brain Res. 112: 363–369.

    PubMed  CAS  Google Scholar 

  • Margolis, R. K., Thomas, M. D., Crockett, C. P., and Margolis, R. U., 1979, Presence of chondroitin sulfate in the neuronal cytoplasm, Proc. Natl. Acad. Sci. USA 76: 1711–1715.

    PubMed  CAS  Google Scholar 

  • Margolis, R. K., Salton, S. R. J., and Margolis, R. U., 1983a, Complex carbohydrates of cultured PC12 pheochromocytoma cells. Effects of nerve growth factor and comparison with neonatal and mature rat brain, J. Biol. Chem. 258: 4110–4117.

    PubMed  CAS  Google Scholar 

  • Margolis, R. K., Salton, S. R. J., and Margolis, R. U., 1983b, Structural features of the nerve growth factor inducible large external glycoprotein of PC12 pheochromocytoma cells and brain, J. Neurochem. 41: 1635–1640.

    PubMed  CAS  Google Scholar 

  • Margolis, R. K., Finne, J., Krusius, T., and Margolis, R. U., 1984, Structural studies on glycoprotein oligosaccharides of chromaffin granule membranes and dopamine ß-hydroxylase, Arch. Biochem. Biophys. 228: 443–449.

    PubMed  CAS  Google Scholar 

  • Margolis, R. K., Greene, L. A., and Margolis, R. U., 1986, Poly(N-acetyllactosaminyl) oligosaccharides in glycoproteins of PC12 pheochromocytoma cells and sympathetic neurons, Biochemistry 25: 3463–3468.

    PubMed  CAS  Google Scholar 

  • Margolis, R. K., Salton, S. R. J., and Margolis, R. U., 1987a, Effects of nerve growth factor-induced differentiation on the heparan sulfate of PC12 pheochromocytoma cells and comparison with developing brain. Arch. Biochem. Biophys. 257: 107–114.

    CAS  Google Scholar 

  • Margolis, R. K., Ripellino, J. A., Goossen B., Steinbrich, R., and Margolis, R. U., 1987b, Occurrence of the Hnk-1 epitope (3-sulfoglucuronic acid) in PC12 pheochromocytoma cells, chromaffin granule membranes, and chondroitin sulfate proteoglycans, Biochem. Biophys. Res. Commun. 145: 1142–1148.

    CAS  Google Scholar 

  • Margolis, R. K., Goossen, B., and Margolis, R. U., 1988, Phosphatidylinositol-anchored glycoproteins of PC12 pheochromocytoma cells and brain, Biochemistry 27: 3454–3458.

    PubMed  CAS  Google Scholar 

  • Margolis, R. U., Margolis, R. K., Chang, L., and Preti, C., 1975, Glycosaminoglycans of brain during development, Biochemistry 14: 85–88.

    PubMed  CAS  Google Scholar 

  • Margolis, R. U., Ledeen, R. W., Sbaschnig-Agler, M., Byrne, M. C., Klein, R. L., Douglas, B. H., II, and Margolis, R. K., 1987, Complex carbohydrate composition of large dense-cored vesicles from sympathetic nerve, J. Neurochem. 49:1839–1844.

    Google Scholar 

  • Margolis, R. U., Fischer-Colbrie, R., and Margolis, R. K., 1988, Poly(N-acetyllactosaminyl) oligosaccharides of chromaffin granule membrane glycoproteins, J. Neurochem. 51: 1819–1824.

    PubMed  CAS  Google Scholar 

  • Matthew, W. D., Greenspan, R. J., Lander, A. D., and Reichardt, L. F., 1985, Immunopurification and characterization of a neuronal heparan sulfate proteoglycan, J. Neurosci. 5: 1842–1850.

    PubMed  CAS  Google Scholar 

  • McGuire, J. C., Greene, L. A., and Furano, A. V., 1978, Ngf stimulates incorporation of fucose or glucosamine into an external glycoprotein in cultured rat PC12 pheochromocytoma cells, Cell 15: 357–365.

    PubMed  CAS  Google Scholar 

  • McKenzie, J. L., Allen, A. K., and Fabre, J. W., 1981, Biochemical characterization including amino acid and carbohydrate compositions of canine and human brain Thy-1 antigen, Biochem. J. 197: 629–636.

    Google Scholar 

  • Merkle, R. K., and Cummings, R. D., 1987, Lectin affinity chromatography of glycopeptides, Methods Enzymol. 138: 232–259.

    PubMed  CAS  Google Scholar 

  • Merkle, R. K., and Heifetz, A., 1984, Enzymatic sulfation of N-glycosidically linked oligosaccharides by endothelial cell membranes, Arch. Biochem. Biophys. 234: 460–467.

    PubMed  CAS  Google Scholar 

  • Merlie, J. P., Sebbane, R., Tzartos, S., and Lindstrom, J., 1982, Inhibition of glycosylation with tunicamycin blocks assembly of newly synthesized acetylcholine receptor subunits in muscle cells, J. Biol. Chem. 257: 2694–2701.

    PubMed  CAS  Google Scholar 

  • Miller, R. R., and Waechter, C. J., 1984, Structural features and some binding properties of proteoheparan sulfate enzymatically labeled by calf brain microsomes, Arch. Biochem. Biophys. 228: 247–257.

    PubMed  CAS  Google Scholar 

  • Moos, M., Tacke, R., Scherer, H., Teplow, D., Früh, K., and Schachner, M., 1988, Neural adhesion molecule Ll as a member of the immunoglobulin superfamily with binding domains similar to fibronectin, Nature 334: 701–703.

    PubMed  CAS  Google Scholar 

  • Morris, J. E., and Ting, Y.-P., 1981, Comparison of proteoglycans extracted by saline and guanidinium chloride from cultured chick retinas, J. Neurochem. 37: 1594–1602.

    PubMed  CAS  Google Scholar 

  • Morris, J. E., Hopwood, J. J., and Dorfman, A., 1977, Biosynthesis of glycosaminoglycans in the developing retina, Dey. Biol. 58: 313–327.

    Google Scholar 

  • Moms, J. E., Ting, Y.-P., and Birkholz-Lambrecht, A., 1984, Low buoyant density proteoglycans from saline and dissociative extracts of embryonic chicken retinas, J. Neurochem. 42: 798–809.

    Google Scholar 

  • Moms, J. E., Yanagishita, M., and Hascall, V., 1987, Proteoglycans synthesized by embryonic chicken retina in culture: Composition and compartmentalization, Arch. Biochem. Biophys. 258: 206–218.

    Google Scholar 

  • Morris, R., 1985, Thy-1 in developing nervous tissue, Dey. Neurosci. 7: 133–160.

    CAS  Google Scholar 

  • Nakanishi, S., 1983, Extracellular matrix during laminar pattern formation of neocortex in normal and reeler mutant mice, Dey. Biol. 95: 305–316.

    CAS  Google Scholar 

  • Needham, L. K., Adler, R., and Hewitt, A. T., 1988, Proteoglycan synthesis in flat cell-free cultures of chick embryo retinal neurons and photoreceptors, Dey. Biol. 126: 304–314.

    CAS  Google Scholar 

  • Obendorf, D., Schwarzenbrunner, U., Fischer-Colbrie, R., Laslop, A., and Winkler, H., 1988, Immunological characterization of a membrane glycoprotein of chromaffin granules: Its presence in endocrine and exocrine tissues, Neuroscience 25: 343–351.

    PubMed  CAS  Google Scholar 

  • Olden, K., Bernard, B. A., Humphries, M. J., Yeo, K.-T., White, S. L., Newton, S. A., Bauer, H. C., and Parent, J. B., 1985, Function of glycoprotein glycans, Trends Biochem. Sci. 10: 78–82.

    CAS  Google Scholar 

  • Osawa, T., and Tsuji, T., 1987, Fractionation and structural assessment of oligosaccharides and glycopeptides by use of immobilized lectins, Annu. Rev. Biochem. 56: 21–42.

    PubMed  CAS  Google Scholar 

  • Parekh, R. B., Tse, A. G. D., Dwek, R. A., Williams, A. F., and Rademacher, T. W., 1987, Tissue-specific N-glycosylation, site-specific oligosaccharide patterns and lentil lectin recognition of rat Thy-1, Embo J. 6: 1233–1244.

    PubMed  CAS  Google Scholar 

  • Porrello, K., and LaVail, M. M. 1986, Immunocytochemical localization of chondroitin sulfates in the interphotoreceptor matrix of the normal and dystrophic rat retina, Curr. Eye Res. 5: 981–994.

    PubMed  CAS  Google Scholar 

  • Porrello, K., Yasumura, D., and LaVail, M. M., 1986, The interphotoreceptor matrix in Rcs rats: Histochemical analysis and correlation with the rate of retinal degeneration, Exp. Eye Res. 43: 413–430.

    PubMed  CAS  Google Scholar 

  • Prives, J., and Bar-Sagi, D., 1983, Effect of tunicamycin, an inhibitor of protein glycosylation, on the biological properties of acetylcholine receptor in cultured muscle cells, J. Biol. Chem. 258: 1775–1780.

    PubMed  CAS  Google Scholar 

  • Pryde, J. G., and Phillips, J. H., 1986, Fractionation of membrane proteins by temperature-induced phase separation in Triton X-114, Biochem. J. 233: 525–533.

    PubMed  CAS  Google Scholar 

  • Ratner, N., Hong, D., Lieberman, M. A., Bunge, R. P., and Glaser, L., 1988, The neuronal cell-surface molecule mitogenic for Schwann cells is a heparin-binding protein, Proc. Natl. Acad. Sci. 85: 6992–6996.

    PubMed  CAS  Google Scholar 

  • Reddy, P., Jacquier, A. C., Abovich, N., Petersen, G., and Rosbash, M., 1986, The period clock locus of D. melanogaster codes for a proteoglycan, Cell 46: 53–61.

    PubMed  CAS  Google Scholar 

  • Richter-Landsberg, C., Greene, L. A., and Shelanski, M. L., 1985, Cell surface Thy-l-cross-reactive glycoprotein in cultured PC12 cells: Modulation of nerve growth factor and association with the cytoskeleton, J. Neurosci. 5: 468–476.

    PubMed  CAS  Google Scholar 

  • Ripellino, J. A., and Margolis, R. U., 1989, Structural properties of the heparan sulfate proteoglycans of brain, J. Neurochem. 52: 807–812.

    PubMed  CAS  Google Scholar 

  • Ripellino, J. A., Klinger, M. M., Margolis, R. U., and Margolis, R. K., 1985, The hyaluronic acid binding region as a specific probe for the localization of hyaluronic acid in tissue sections. Application to chick embryo and rat brain, J. Histochem. Cytochem. 33: 1060–1066.

    PubMed  CAS  Google Scholar 

  • Ripellino, J. A., Bailo, M., Margolis, R. U., and Margolis, R. K., 1988, Light and electron microscopic studies on the localization of hyaluronic acid in developing rat cerebellum, J. Cell Biol. 106: 845–855.

    PubMed  CAS  Google Scholar 

  • Ripellino, J. A., Margolis, R. U., and Margolis, R. K., 1989a, Oligosaccharide composition, localization, and developmental changes of a brain–specific (F3–87–8) glycoprotein, J. Neurochem.,in press

    Google Scholar 

  • Ripellino, J. A., Margolis, R. U., and Margolis, R. K., 1989b, Immunoelectron microscopic localization of hyaluronic acid binding region and link protein epitopes in brain, J. Cell Biol.,in press.

    Google Scholar 

  • Rogers, S. L., McCarthy, J. B., Palm, S. L., Furcht, L. T., and Letourneau, P. C., 1985, Neuron-specific interactions with two neurite-promoting fragments of fibronectin, J. Neurosci. 5: 369–378.

    PubMed  CAS  Google Scholar 

  • Roth, J., Taatjes, D. J., Bitter-Suermann, D., and Finne, J., 1987, Polysialic acid units are spatially and temporally expressed in developing postnatal rat kidney, Proc. Natl. Acad. Sci. USA 84: 1969–1973.

    PubMed  CAS  Google Scholar 

  • Roussel, P., Lamblin, G., Degand, P., Walker-Nasir, E., and Jeanloz, R. W., 1975, Heterogeneity of the carbohydrate chains of sulfated bronchial glycoproteins isolated from a patient suffering from cystic fibrosis, J. Biol. Chem. 250: 2114–2122.

    PubMed  CAS  Google Scholar 

  • Sajovic, P., Kouvelas, E., and Trenkner, E., 1986, Probable identity of Nile glycoprotein and the highmolecular-weight component of Ll antigen, J. Neurochem. 47: 541–546.

    PubMed  CAS  Google Scholar 

  • Salton, S. R. J., Margolis, R. U., and Margolis, R. K., 1983a, Release of chromaffin granule glycoproteins and proteoglycans from potassium-stimulated PC 12 pheochromocytoma cells, J. Neurochem. 41: 1165–1170.

    CAS  Google Scholar 

  • Salton, S. R. J., Richter-Landsberg, C., Greene, L. A., and Shelanski, M. L., 1983b, Nerve growth factor-inducible large external (Nile) glycoprotein: Studies of a central and peripheral neuronal marker, J. Neurosci. 3: 441–454.

    PubMed  CAS  Google Scholar 

  • Schauer, R. (ed.), 1982, Sialic Acids—Chemistry, Metabolism and Function, Springer-Verlag, Berlin.

    Google Scholar 

  • Schubert, D., Schroeder, R., LaCorbiere, M., Saitoh, T., and Cole, G., 1988, Amyloid ß protein precursor is possibly a heparan sulfate proteoglycan core protein, Science 241: 223–226.

    PubMed  CAS  Google Scholar 

  • Seiger, A., Almqvist, P., Granholm, A.-C., and Olson, L., 1986, On the localization of Thy-1-like immunoreactivity in the rodent and human nervous system, Med. Biol. 64: 109–117.

    PubMed  CAS  Google Scholar 

  • Selkoe, D. J., Podlisny, M. B., Joachim, C. L., Vickers, E. A., Lee, G., Fritz, L. C., and Oltersdorf, T., 1988, 13-Amyloid precursor protein of Alzheimer disease occurs as 110- to 135-kilodalton membrane-associated proteins in neural and nonneural tissues, Proc. Natl. Acad. Sci. USA 85: 7341–7345.

    PubMed  CAS  Google Scholar 

  • Simpson, D. L., Thome, D. R., and Loh, H. H., 1976, Sulfated glycoproteins, glycolipids and glycosaminoglycans from synaptic plasma and myelin membranes: Isolation and characterization of sulfated glycopepetides, Biochemistry 15: 5449–5457.

    PubMed  CAS  Google Scholar 

  • Slomiany, B. L., and Meyer, K., 1972, Isolation and structural studies of sulfated glycoproteins of hog gastric mucosa, J. Biol. Chem. 247: 5062–5070.

    PubMed  CAS  Google Scholar 

  • Spillmann, D., and Finne, J., 1987, Poly-N-acetyllactosamine glycans of cellular glycoproteins: Predominance of linear chains in mouse neuroblastoma and rat pheochromocytoma cell lines, J. Neurochem. 49: 874–883.

    PubMed  CAS  Google Scholar 

  • Stallcup, W. B., and Beasley, L., 1985, Involvement of the nerve growth factor-inducible large external glycoprotein (Nile) in neurite fasciculation in primary cultures of rat brain, Proc. Natl. Acad. Sci. USA 82: 1276–1280.

    PubMed  CAS  Google Scholar 

  • Stallcup, W. B., and Beasley, L., 1987, Bipotential glial precursor cells of the optic nerve express the NG2 proteoglycan, J. Neurosci. 7: 2737–2744.

    PubMed  CAS  Google Scholar 

  • Stallcup, W. B., Beasley, L., and Levine, J., 1983, Cell-surface molecules that characterize different stages in the development of cerebellar intemeurons, Cold Spring Harbor Symp. Quant. Biol. 48: 761–774.

    PubMed  CAS  Google Scholar 

  • Stallcup, W. B., Beasley, L. L., and Levine, J. M., 1985, Antibody against nerve growth factor-inducible large external (Nile) glycoprotein labels nerve fiber tracts in the developing rat nervous system, J. Neurosci. 5: 1090–1101.

    PubMed  CAS  Google Scholar 

  • Sweeley, C. C., and Nunez, H. A., 1985, Structural analysis of glycoconjugates by mass spectrometry and nuclear magnetic resonance spectroscopy, Annu. Rev. Biochem. 54: 765–801.

    PubMed  CAS  Google Scholar 

  • Tan, S.-S., Crossin, K. L., Hoffman, S., and Edelman, G. M., 1987, Asymmetric expression in somites of cytotactin and its proteoglycan ligand is correlated with neural crest cell distribution, Proc. Natl. Acad. Sci. USA 84: 7977–7981.

    PubMed  CAS  Google Scholar 

  • Tisdale, E. J., and Tartakoff, A. M., 1988, Extensive labeling with [3H]ethanolamine of a hydrophilic protein of animal cells, J. Biol. Chem. 263: 8244–8252.

    PubMed  CAS  Google Scholar 

  • Tulsiani, D. R. P., and Touster, O., 1987, Substrate specificities of rat kidney lysosomal and cytosolic a-nmannosidases and effects of swainsonine suggest a role of the cytosolic enzyme in glycoprotein catabolism, J. Biol. Chem. 262: 6506–6514.

    PubMed  CAS  Google Scholar 

  • Waechter, C. J., Schmidt, J. W., and Catterall, W. A., 1983, Glycosylation is required for maintenance of functional sodium channels in neuroblastoma cells, J. Biol. Chem. 258: 5117–5123.

    PubMed  CAS  Google Scholar 

  • Weber, R. J., Hill, J. M., and Pert, C. B., 1988, Regional distribution and density of Thy 1.1 in rat brain and its relation to subpopulations of neurons, J. Neuroimmunol. 17: 137–145.

    Google Scholar 

  • West, C. M., 1986, Current ideas on the significance of protein glycosylation, Mol. Cell. Biochem. 72: 320.

    Google Scholar 

  • Wieland, F., Paul, G., and Sumper, M., 1985, Halobacterial flagellins are sulfated glycoproteins, J. Biol. Chem. 260: 15180–15185.

    PubMed  CAS  Google Scholar 

  • Wight, T. N., and Mecham, R. P. (eds.), 1987. Biology of Proteoglycans, Academic Press, New York.

    Google Scholar 

  • Williams, A. F., and Barclay, A. N., 1988, The immunoglobulin superfamily—Domains for cell surface recognition, Annu. Rev. lmmunol. 6: 381–406.

    Google Scholar 

  • Williams, A. F., and Gagnon, J., 1982, Neuronal cell Thy-1 glycoprotein: Homology with immunoglobulin, Science 216: 696–703.

    PubMed  CAS  Google Scholar 

  • Winkler, H., Apps, D. K., and Fischer-Colbrie, R., 1986, The molecular function of adrenal chromaffin granules: Established facts and unresolved topics, Neuroscience 18: 261–290.

    Google Scholar 

  • Yamashita, K., Hitoi, A., and Kobata, A., 1983a, Structural determinants of Phaseolus vulgaris erythroagglutinating lectin for oligosaccharides, J. Biol. Chem. 258: 14753–14755.

    CAS  Google Scholar 

  • Yamashita, K., Ueda, I., and Kobata, A., 1983b, Sulfated asparagine-linked sugar chains of hen egg albumin, J. Biol. Chem. 258: 14144–14147.

    PubMed  CAS  Google Scholar 

  • Zanini, A., Giannattasio, G., Nussdorfer, G., Margolis, R. K., Margolis, R. U., and Meldolesi, J., 1980, Molecular organization of prolactin granules. H. Characterization of glycosaminoglycans and glycoproteins of the bovine prolactin granule matrix, J. Cell Biol. 86: 260–272.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacology, State University of New York, Health Science Center at Brooklyn, Brooklyn, New York, 11203, USA

    Renée K. Margolis

  2. Department of Pharmacology, New York University Medical Center, New York, New York, 10016, USA

    Richard U. Margolis

Authors
  1. Renée K. Margolis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Richard U. Margolis
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Pharmacology, New York University Medical Center, 10016, New York, New York, USA

    Richard U. Margolis

  2. Department of Pharmacology, State University of New York, Health Science Center at Brooklyn, 11203, Brooklyn, New York, USA

    Renée K. Margolis

Rights and permissions

Reprints and permissions

Copyright information

© 1989 Springer Science+Business Media New York

About this chapter

Cite this chapter

Margolis, R.K., Margolis, R.U. (1989). Structure and Localization of Glycoproteins and Proteoglycans. In: Margolis, R.U., Margolis, R.K. (eds) Neurobiology of Glycoconjugates. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5955-6_3

Download citation

  • DOI: https://doi.org/10.1007/978-1-4757-5955-6_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-5957-0

  • Online ISBN: 978-1-4757-5955-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation