Cell Lineage Studies in Avian Neural Crest Ontogeny

  • Anne Baroffio
  • Elisabeth Dupin
  • Nicole M. Le Douarin
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 265)


The neural crest of the Vertebrate embryo is a transitory structure arising during the closure of the neural tube and lying on its dorsal aspect. From it originates most of the peripheral nervous system (PNS), including neurons of all the sympathetic, parasympathetic and enteric ganglia and of the majority of sensory ganglia as well as glial cells of all these ganglia. In addition, the neural crest gives rise to Schwann cells of the peripheral nerves and to many other cell types, such as endocrine cells (e. g. adrenomedullary and calcitoninproducing cells), melanocytes and, in the head region, to the mesectoderm (see Le Douarin, 1982, for review).


Dorsal Root Ganglion Tyrosine Hydroxylase Neural Tube Neural Crest Chromaffin Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Abo, T., and Balch, C.M., 1981, A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1), J. Immunol. 127: 1024.Google Scholar
  2. Anderson, D.J., and Axel, R., 1986, A bipotential neuroendocrine precursor whose choice of cell fate is determined by NGF and glucocorticoids, Cell 47: 1079.Google Scholar
  3. Ayer-Le Lièvre, C., and Le Douarin, N.M., 1982, The early development of cranial sensory ganglia and the potentialities of their component cells studied in quail-chick chimeras, Dev. Biol. 94:291.Google Scholar
  4. Bader, C.R., Bertrand, D., Dupin, E., and Kato, A.C., 1983, Development of electrical membrane properties in cultured avian neural crest. Nature, 305: 808.CrossRefGoogle Scholar
  5. Barald, K.F., 1982, Monoclonal antibodies to embryonic neurons. Cell-specific markers for chick ciliary ganglion. in: “Neuronal Development”, N.C. Spitzer, ed, Plenum Press, New YorkGoogle Scholar
  6. Barasch, J.M., Mackey, H., Tamir, H., Nunez, E.A., and Gershon, M.D., 1987, Induction of a neural phenotype in a serotonergic endocrine cell derived from the neural crest, J. Neurosci. 7:2874.Google Scholar
  7. Barbu, M., Ziller, C., Rong, P.M., and Le Douarin, N.M., 1986, Heterogeneity in migrating neural crest cells revealed by a monoclonal antibody, J. Neurosci. 6:2215.Google Scholar
  8. Baroffio, A., Dupin, E., and Le Douarin, N.M., 1988, Clone-forming ability and differentiation potential of migratory neural crest cells, Proc. Natl. Acad. Sci. USA 85:5325.Google Scholar
  9. Black, I.B., 1982, Stages of neurotransmitter development in autonomic neurons, Science, 215: 1198.CrossRefGoogle Scholar
  10. Bohn, M.C., Kessler, J.A., Adler, J.E., Markey, K., Goldstein, M., and Black, I.B., 1984, Simultaneous expression of the SP-peptidergic and noradrenergic phenotypes in rat sympathetic neurons, Brain Res., 298: 378.Google Scholar
  11. Bronner-Fraser, M., and Fraser, S.E., 1988, Cell lineage analysis reveals multipotency of some avian neural crest cells, Nature 335: 161.Google Scholar
  12. Cepko, C., 1988, Immortalization of neural cells via oncogene transduction, Trends in Neurosci 11: 6.Google Scholar
  13. Christie, D.S., Forbes, M.E., and Maxwell, G.D., 1987, Phenotypic properties of catecholamine-positive cells that differentiate in avian neural crest cultures, J. Neurosci. 7:3749.Google Scholar
  14. Ciment, G., and Weston, J.A., 1982, Early appearance in neural crest and crest-derived cells of a antigenic determinant present in avian neurons, Dev. Biol. 93:355.Google Scholar
  15. Cohen, A.M., and Konigsberg, I.R., 1975, A clonal approach to the problem of neural crest determination, Dev. Biol. 46:262.Google Scholar
  16. Cohen, A.M., 1977, Independent expression of the adrenergic phenotype by neural crest cells in vitro, Proc. Natl. Acad. Sci. USA 74:2899.Google Scholar
  17. Doupe, A.J., Landis, S.C., and Patterson, P.H., 1985a, Environmental influences in the development of neural crest derivatives. glucocorticoids, growth factors and chromaffin cell plasticity, J. Neurosci. 5: 2119.Google Scholar
  18. Coupe, A.J., Patterson, P.H., and Landis, S.C., 1985b, Small intensely fluorescent cells in culture: role of glucocorticoids and growth factors in their development and interconversions with other neural crest derivatives, J. Neurosci. 5:2143.Google Scholar
  19. Ernsberger, U., and Rohrer, H., 1988, Neuronal precursor cells in chick dorsal root ganglia: differentiation and survival in vitro, Dev.Biol. 126: 420.Google Scholar
  20. Fauquet, M., and Ziller, C., 1988, Tyrosine hydroxylase-positive cells, differentiated in vitro from neural crest, exhibit neuronal features and peptide-like immunoreactivities, J. Histochem. Cytochem. (submitted).Google Scholar
  21. Fontaine-Pérus, J., 1984,. Development of VIP in the peripheral nervous system of avian embryo, Pe tides, 5: 195.Google Scholar
  22. Garcia-Arraras, J.E.,Chanconie, M., Ziller, C., and Fauquet, M., 1987, In vivo and in vitro expression of vasoactive intestinal polypeptide-like immunoreactivity by neural crest derivatives, Dev. Brain Res. 33:255.Google Scholar
  23. Girdelstone, J., and Weston, J.A., 1985, Identification of early neuronal subpopulations in avian neural crest cultures, Dev. Biol. 109:274.Google Scholar
  24. Hall, B.K., and Tremaine, R., 1979, Ability of neural crest cells from the embryonic chick to differentiate into cartilage before their migration away from the neural tube, Anat. Rec. 194:469.Google Scholar
  25. Howard, M.J., and Bronner-Fraser, M., 1985, The influence of neural tube-derived factors on differentiation of neural crest cells in vitro. I. Histochemical study on the appearance of adrenergic cells, J. Neurosci. 5:3302.Google Scholar
  26. Hughes, S.M., Lillien, L.E., Raff, M.C., Rohrer, H., and Sendtner, M., 1988, Ciliary neurotrophic factor induces type-2 astrocyte differentiation in culture, Nature 335: 70.Google Scholar
  27. Kalcheim, C., and Le Douarin, N.M., 1986, Requirement of a neural tube signal for the differentiation of neural crest cells into dorsal root ganglia, Dev. Biol. 116:451.Google Scholar
  28. Kalcheim, C., Barde, Y.-A., Thoenen, H., and Le Douarin, N.M., 1987, In vivo effect of brain-derived neurotrophic factor on the survival of neural crest precursor cells of the dorsal root ganglia, EMBO J. 6:2871.Google Scholar
  29. Kessler, J.A., Adler, J.E., Bohn, M.C., and Black, I.B., 1981, Substance P in principal sympathetic neurons: regulation by impulse activity. Science 214: 335.Google Scholar
  30. Le Douarin, N.M. 1982, “The Neural Crest”, Cambridge University Press, Cambridge.Google Scholar
  31. Le Douarin, N.M., and Teillet, M.-A., 1974, Experimental analysis of the migration and differentiation of neuroblasts of the autonomic nervous system and of neurectodermal mesenchymal derivatives, using a biological cell marking technique, Dev. Biol. 41:162.Google Scholar
  32. Le Douarin, N.M., 1986, Cell line segregation during peripheral nervous system ontogeny, Science 231: 1515.Google Scholar
  33. Loring, J., Glimelius, B., and Weston, J.A.,1982, Extracellular matrix materials influence quail neural crest cell differentiation in vitro. Dev. Biol. 90:165.Google Scholar
  34. Maxwell, G.D., and Forbes, E., 1987, Exogenous basement membrane-like matrix stimulates adrenergic development in avian neural crest cultures, Development 101: 767.Google Scholar
  35. New, H.V., and Mudge, A.W., 1986, Distribution and ontogeny of SP, CGRP, SOM and VIP in chick sensory and sympathetic ganglia, Dev. Biol. 116: 337.CrossRefGoogle Scholar
  36. Payette, R.F., Bennett, G.S., and Gershon, M.D., 1984, Neurofilament expression in vagal neural crest-derived precursors of enteric neurons, Dev. Biol. 105:273.Google Scholar
  37. Price, J., Turner, D., and Cepko, C., 1987, Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer, Proc. Natl. Acad. Sci. USA 84:156.Google Scholar
  38. Raff, M.C., Miller, R.H., and Noble, M., 1983, A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending on culture medium, Nature 303: 390.Google Scholar
  39. Raff, M.C., Lillien, L.E., Richardson, W.D., Burne, J.F., and Noble, M.D., 1988, Platelet-derived growth factor from astrocytes drives the clock that times oligodendrocyte development in culture, Nature 333: 562.Google Scholar
  40. Rheinwald, J.G., and Green, H., 1975, Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells, Cell, 6: 331.CrossRefGoogle Scholar
  41. Rohrer, H., Henke-Fahle, S., El-Sharkawy, T., Lux, H.D., and ThoenenGoogle Scholar
  42. H., 1985, Progenitor cells from embryonic chick dorsal root ganglia differentiate in vitro to neurons biochemical and electrophysiological evidence, EMBO J. 4: 1709.Google Scholar
  43. Rohrer, H., Acheson, A.L., Thibault, J., and Thoenen, H., 1986, Developmental potential of quail dorsal root ganglion cells analyzed in vitro and in vivo, J. Neurosci. 6:2616.Google Scholar
  44. Schweizer, G., Ayer-Le Lièvre, C., and Le Douarin, N.M., 1983, Restrictions of developmental capacities in the dorsal root ganglia during the course of development, Cell Diff. 13: 200.Google Scholar
  45. Sieber-Blum, M., and Cohen, A.M., 1980, Clonal analysis of quail neural crest cells: they are pluripotent and differentiate in vitro in the absence of non crest cells, Dev. Biol. 80:96.Google Scholar
  46. Sieber-Blum, M., Kumar, S.R., and Riley, D.A., 1988, In vitro differentiation of quail neural crest cells into sensory-like neuroblasts, Dev. Brain Res. 39:69.Google Scholar
  47. Sulston, J.E., Schierenberg, E., White, J.G., and Thomson, J.N., 1983, The embryonic cell lineage of the nematode Caenorhabditis elegans, Dev. Biol. 100:64.Google Scholar
  48. Teillet, M.A., and Le Douarin, N.M., 1983, Consequences of neural tube and notochord excision on the development of the peripheral nervous system in the chick embryo, Dev. Biol. 98:192.Google Scholar
  49. Todaro, G.J., and Green, H., 1963, Quantitative studies of mouse embryo cells in culture and their development into cell line, J. Cell Biol. 17:299.Google Scholar
  50. Tucker, G.C., Aoyama, H., Lipinski, M., Tursz, T., and Thiery, J.P., 1984, Identical reactivity of monoclonal antibodies HNK-1 and NC-1: conservation in vertebrates on cells derived from the neural primordium and on some leukocytes, Cell Diff. 14: 223.Google Scholar
  51. Turner, D.L., and Cepko, C.L., 1987, A common progenitor for neurons and glia persists in rat retina late in development, Nature 328: 131.Google Scholar
  52. Unsicker, K., Krisch, B., Otten, U., and Thoenen, H., 1978, Nerve growth factor-induced fiber outgrowth from isolated rat adrenal chromaffin cells: impairment by glucocorticoids, Proc. Natl. Acad. Sci. USA 75:3498.Google Scholar
  53. Wetts, R., and Fraser, S.E., 1988, Multipotent precursors can give rise to all major cell types of the frog retina, Science 239: 1142.Google Scholar
  54. Xue, Z.G., Smith, J., and Le Douarin, N.M., 1985, Differentiation of catecholaminergic cells in cultures of embryonic avian sensory ganglia, Proc. Natl. Acad. Sci. USA 82:8800.Google Scholar
  55. Xue, Z.G., Smith, J., and Le Douarin, N.M., 1987, Developmental capacities of avian embryonic dorsal root ganglion cells: neuropeptides and tyrosine hydroxylase in dissociated cell cultures, Dev. Brain Res. 34:99.Google Scholar
  56. Xue, Z.G., and Smith, J., 1988a, High affinity uptake of noradrenaline in quail dorsal root ganglion cells that express tyrosine hydroxylase immunoreactivity in vitro, J. Neurosci. 8:806.Google Scholar
  57. Xue, Z.G., Le Douarin, N.M., and Smith, J., 1988b, Insulin and insulin-like growth factor-I trigger the differentiation of catecholaminergic precursors in cultures of dorsal root ganglia, Cell Diff. 25: 1.Google Scholar
  58. Ziller, C., Dupin, E., Brazeau, P., Paulin, D., and Le Douarin, N.M., 1983, Early segregation of a neuronal precursor cell line in the neural crest as revealed by culture in a chemically defined medium, Cell, 32: 627.CrossRefGoogle Scholar
  59. Ziller, C., Fauquet, M., Kalcheim, C., Smith, J., and Le Douarin, N.M., 1987, Cell lineages in peripheral nervous sytem ontogeny: medium-induced modulation of neuronal phenotypic expression in neural crest cell cultures, Dev. Biol. 120:101.Google Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Anne Baroffio
    • 1
  • Elisabeth Dupin
    • 1
  • Nicole M. Le Douarin
    • 1
  1. 1.Institut d’Embryologie cellulaire et moléculaire du CNRS et du Collège de FranceNogent-sur-Marne CedexFrance

Personalised recommendations