Serotonin and Morphogenesis in the Cultured Mouse Embryo

  • Dana L. Shuey
  • Mark Yavarone
  • Thomas W. Sadler
  • Jean M. Lauder
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 265)


Morphogenesis is the process whereby embryonic tissues and organs are formed through cell movements, changes in cell shape and cell-cell interactions. Not only are morphogenetic cell movements important in the establishment of tissue form, but they are also believed to play an important role in bringing interactive tissues into contact. For example, it has been demonstrated that the dorsal lip of the blastopore migrating beneath the overlying ectoderm is responsible for induction of the nervous system during gastrulation (Spemann & Mangold, 1924).


Neural Crest Neural Crest Cell Overlie Surface Epithelium Endocardial Cushion Craniofacial Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bee, J. and Thorogood, P. (1980) The role of tissue interactions in the skeletogenic differentiation of avian neural crest cells. Dev Biol. 78: 47–62.CrossRefGoogle Scholar
  2. Burden, R.W. and Lawrence, I.E. (1973) Presence of biogenic amines in early rat development. Am J Anat. 136: 251–257.CrossRefGoogle Scholar
  3. Buznikov, G.A. (1980) Biogenic monoamines and acetylcholine in protozoa and metazoan embryos. In J. Salanki and T.M. Turpaev (Eds.), Neurotransmitters, Comparative Aspects Akademiai Kiado, Budapest, pp. 7–29.Google Scholar
  4. Buznikov, G.A. (1984) The action of neurotransmitters and related substances on early embryogenesis. Pharmac Ther. 25: 23–59.CrossRefGoogle Scholar
  5. Buznikov, G.A., Chudakova, I.V. and Zvezdina, N.D. (1964) The role of neurohumours in early embryogenesis I Serotonin content of developing embryos of sea urchin and loach. J Embryol Exp Morph. 12: 563–573.Google Scholar
  6. Buznikov, G.A., Kost, A.N., Kucherova, N.F., Mndzhoyan, A.L., Suvorov, N.N. and Berdysheva, L.V. (1970) The role of neurohumours in early embryogenesis III Pharmacological analysis of the role of neurohumors in cleavage divisions. J Embryol Exp Morph. 23: 549–569.Google Scholar
  7. Buznikov, G.A., Malchenko, L.A., Rakic, L., Kovacevic, N., Markova, L.N., Salimova, N.B., and Volina E.V. (1984) Sensitivity of starfish oocytes and whole, half and enucleated embryos to cytotoxic neuropharmacological drugs. Comp Biochem Physiol. 78C: 197–201.CrossRefGoogle Scholar
  8. Buznikov, G.A., Mileusnic, R., Yurovskaya, M.A. and Rakic, L. (1984) Effect of calcium ionophore A23187 on the sensitivity of early sea urchin embryos to cytotoxic neuropharmacological drugs. Comp Biochem Physiol. 79C: 425–427.CrossRefGoogle Scholar
  9. Buznikov, G.A. and Smukler, Y.B. (1981) The possible role of “prenervous” neurotransmitters in cellular interactions of early embryogenesis: a hypothesis. Neurochem Res. 6: 55–69.CrossRefGoogle Scholar
  10. Emanuelsson, H. (1974) Localization of serotonin in cleavage embryos of Ophryotrocha labronica La Greca and Bacci. Roux’ Arch. Entw.-mech., 175: 253–271.CrossRefGoogle Scholar
  11. Fitzharris, T.P. and Markwald, R.R. (1982). Cellular migration through the cardiac jelly matrix: a stereoanalysis by high voltage electron microscopy. Dev Biol. 92: 315–329.CrossRefGoogle Scholar
  12. Gustafson, T. and Toneby, M. (1970) On the role of serotonin and acetylcholine in sea urchin morphogenesis. Ex. Cell Res. 62: 107–117.Google Scholar
  13. Gustafson T. and Toneby, M. (1971) How genes control morphogenesis. Am Sci. 59: 452–462.Google Scholar
  14. Hall, B.K. (1980) Tissue interactions and the initiation of osteogenesis and chondrogenesis in the neural crest-derived mandibular skeleton of the embryonic mouse as seen in isolated murine tissues and in recombinations of murine and avian tissues. J Embryol exp Morph. 58: 251–264.Google Scholar
  15. Holtfreter, J. (1968) On mesenchyme and epithelia in inductive and morphogenetic processes. In R. Fleischmajer and R.E. Billingham (Eds.), Epithelial-Mesenchymal Interactions. Williams and Wilkins, Baltimore, pp. 1–30.Google Scholar
  16. Johnston, M.C. (1975) The neural crest in abnormalities of the face and brain. Birth Defects Original Article Series 11 (7): 1–18.Google Scholar
  17. Kirschgessner, A.L., Liu, K.P., Gershon, M.D. and Tamir H. (1987) Co-localization of serotonin with specific serotonin binding proteins in serotonergic neurons of the rat brain and spinal cord. Anat Rec. 218: 73A.Google Scholar
  18. Landau, M.A., Buznikov, G.A., Kabandin, A.S., Teplitz, N.A., and Chernilovskaya, P.E. (1981) The sensitivity of sea urchin embryos to cytotoxic neuropharmacological drugs the correlations between activity and lipophility of indole and benzole derivatives. Comp Biochem Physiol. 69C: 359–366.Google Scholar
  19. Lauder, J.M., Lindemann Shuey, D., Thomas, R., Yavarone, M., and Sadler, T.W. (1989) Serotonin in the ectoplacental cone of the cultured mouse embryo. Development (submitted).Google Scholar
  20. Lauder, J.M., Tamir, H., and Sadler T.W. (1988) Serotonin and morphogenesis I Sites of serotonin uptake and binding protein immunoreactivity in the midgestation mouse embryo. Development 102: 709–720.Google Scholar
  21. Lauder, J.M. Tamir, H. and Sadler, T.W. (1987) Sites of serotonin uptake and binding protein immunoreactivity in the cultured mouse embryo roles in morphogenesis? Soc Neurosci Abstr. 13: 254.Google Scholar
  22. Lauder, J.M. and Zimmerman, E. (1988) Sites of serotonin uptake in the epithelium of the developing mouse palate, oral cavity and face: possible roles in morphogenesis? J Craniofac Genet Dev Biol. 8: 265–276.Google Scholar
  23. LeDouarin, N.M. (1982) The Neural Crest. Cambridge University Press, Cambridge.Google Scholar
  24. Liu, K.P., Gershon, M.D. and Tamir, H. (1985) Identification, purification, and characterization of two forms of serotonin binding protein from rat brain. J Neurochem. 44:1289–1301Google Scholar
  25. Liu, K.P., Gershon, M.D. and Tamir, H. (1985) Identification, purification, and characterization of two forms of serotonin binding protein from rat brain. J Neurochem. 44:1289–1301.Google Scholar
  26. Liu, K.P., Hsuing, S.C., Kirschgessner, A.L., Gershon, M.D. and Tamir, H. (1987) Co-localization of serotonin with serotonin binding proteins in the rat CNS. J. Neurochem. ( Suppl. ) 48: 63.Google Scholar
  27. Markwald, R.R., Fitzharris, T.P. and Manasek, F.J. (1976) Structural development of endocardial cushions. Am J Anat. 148: 85–93.CrossRefGoogle Scholar
  28. Newgreen, D.F. (1985) Control of the timing of commencement of migration of embryonic neural crest cells. Expl Biol Med. 10: 209–221.Google Scholar
  29. Newgreen, D.F., Allan, I.J., Young, H.M. and Southwell, B.R. (1981) Accumulation of exogenous catecholamines in the neural tube and non-neural tissues of the early fowl embryo Correlation with morphogenetic movements. W Roux’s Arch. 190: 320–330.CrossRefGoogle Scholar
  30. Nichols, D.H. (1981) Neural crest formation in the head of the mouse embryo as observed using a new histological technique. J Embryol exp Morph. 64: 105–120.Google Scholar
  31. Nichols, D.H. (1986) Formation and distribution of neural crest mesenchyme to the first pharyngeal arch region of the mouse embryo. Am J Anat. 176: 221–231.CrossRefGoogle Scholar
  32. Palen, K., Thorneby, L. and Emanuelsson, H. (1979) Effects of serotonin and serotonin antagonists on chick embryogenesis. W Roux’s Arch. 187: 89–103.CrossRefGoogle Scholar
  33. Pratt, R.M., Larsen, M.A. and Johnston, M.C. (1975) Migration of cranial neural crest cells in a cell-free hyaluronate-rich matrix. Devl Biol. 44: 298–305.CrossRefGoogle Scholar
  34. Renaud, F., Parisi, E., Capasso, A. and DePrisco, P. (1983) On the role of serotonin and 5-methoxytryptamine in the regulation of cell division in sea urchin eggs. Devl Biol. 98: 37–46.CrossRefGoogle Scholar
  35. Shmukler, Y.B., Buznikov, G.A., Grigoryev, N.G. and Malchenko, L.A. (1984) Effect of cyclic nucleotides on sensitivity of early sea urchin embryos to cytotoxic.Google Scholar
  36. Spemann, H. and Mangold, H. (1924) Uber Induktion von Embryonalanlagen durch Implantation artfremder Organisatoren. Arch Mik Ant Entw-Mech. 100: 599–638.Google Scholar
  37. Tamir, H. (1983) Serotonin-binding protein: function in synaptic vesicles. Trans NY Acad Sci. 41: 237–242.CrossRefGoogle Scholar
  38. Thorogood, P. (1981) Neural crest cells and skeletogenesis in vertebrate embryos. Histochem. J. 13: 631–642.Google Scholar
  39. Twitty, V. (1955) Eye. In B.H. Willier, P.A. Weiss, and V. Hamburger (Eds.), Analysis of Development. W.B. Saunders, Philadelphia and London, pp. 402–414.Google Scholar
  40. Venkatasubramanian, K. and Zimmerman, E.F. (1983) Palate cell motility and substrate interaction. J Craniofac Genet Dev Biol. 3: 143–157.Google Scholar
  41. Wallace, J.A. (1982) Monoamines in the early chick embryo: demonstration of serotonin synthesis and the regional distribution of serotonin-concentrating cells during morphogenesis. Am J Anat. 165: 261–276.CrossRefGoogle Scholar
  42. Wee, E.L., Babiarz, B.S., Zimmerman, S. and Zimmerman E.F. (1979) Palate morphogenesis IV. Effects of serotonin and its antagonists on rotation in embryo culture. J Embryol Exp Morph. 53: 75–90.Google Scholar
  43. Zimmerman, E.F., Clark, R.L., Ganguli, S. and Venkatasubramanian, K. (1983) Serotonin regulation of palatal cell motility and metabolism. J Craniofac Genet Dev Biol. 3: 371–385.Google Scholar
  44. Zimmerman, E.F. and Lauder, J.M. (1987) Sites of serotonin uptake in the epithelium of the developing mouse palate, oral cavity and face possible role in morphogenesis? Teratology 35: 39A.Google Scholar
  45. Zimmerman, E.F. and Wee, E.L. (1984) Role of neurotransmitters in palate development. In E.F. Zimmerman (Ed.), Current Topics in Developmental Biology: Vol. 19, Palate Development. Academic Press, New York, pp. 37–63.Google Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Dana L. Shuey
    • 1
  • Mark Yavarone
    • 2
  • Thomas W. Sadler
    • 2
  • Jean M. Lauder
    • 1
    • 2
  1. 1.Curriculum in ToxicologyUniversity of North Carolina, School of MedicineChapel HillUSA
  2. 2.Department of Cell Biology and AnatomyUniversity of North Carolina, School of MedicineChapel HillUSA

Personalised recommendations